Mobile phase optimisation for oligonucleotide analytical methods by LC-MS

The use of alkylamine ion pair and perfluorinated alcohols in the analysis of oligonucleotides by LC-MS techniques has been the mainstay in characterisation and quantitation methods for synthetic oligonucleotides for decades. Often, analytical chemists default to relatively high concentrations of both, but how much ion pair is needed for optimal chromatographic performance and how much does perfluorinated alcohol concentration contribute to electrospray ionisation efficiency?

In this presentation, we provide an overview of nucleic acid therapeutics and discuss the chromatographic challenges associated with oligonucleotides. We also explore modulation of the mobile phase additives alkylamine ion pair and perfluorinated alcohols. Finally, we will show how optimisation of mobile phase can improve not only chromatographic performance and MS sensitivity, but may also directly impact MS data acquisition, thus facilitating data analysis.

Presenter: Brian Rivera (Senior Product Manager- Biologics at Phenomenex)