Improved Peptide Mapping of Biotherapeutics

Peptide mapping for biotherapeutic proteins relies on protein digestion which includes the enzymatic treatment of a protein to produce peptide fragments to further characterize specific peptide regions and modifications.

The results of protein digests are essential for protein structural characterization, protein identification, and protein modification monitoring including post-translation modifications, product related impurities, and multi-attribute monitoring (MAM) methods. However, sample preparation for peptide mapping is complex, laborious and often with long digestion times.

In this webinar, we will discuss an automatable sample preparation protocol for peptide mapping that provides a reproducible tryptic protein digest developed for regulated and research peptide mapping applications.

Presenter: Leslie Napoletano (Principal Product Marketing Manager, Consumables & Lab Automation-Biopharm, Waters Corporation)

Leslie has been with Waters since 2012 providing support and education to scientists in New Jersey with Waters chemistry consumables including sample preparation, column selection and delivering educational seminars to customers. More recently in 2021, Leslie has moved into the Product Marketing group focusing on bringing on new consumable products for protein characterization. Prior to joining Waters, Leslie worked as a scientist in protein characterization in the pre-clinical development of monoclonal antibodies at Merck and prior to that at Teva as a R&D chemist.