To Isolation and Beyond: A New Mixed Mode Column Approach for PFAS Chromatography

Typical PFAS methods include 18 to 40 individual legacy, short to long chain PFAS compounds. However, as the long chain PFAS have been phased out of industrial uses, they were replaced by short and ultra-short chain versions with similar chemistries, environmental persistence, and toxicological impact. As we continue to assess PFAS impacts in the environment, methods for identifying and quantifying the short and ultra-short chain PFAS are also needed.

Chromatography methods using common reverse phase columns, however, do not retain the ultra-short chain PFAS, so these are not usually included in the current quantitative methods.

We will explore how we can take advantage of the mixed mode chemistry of PFAS molecules to routinely detect and quantify a broad scope of PFAS, including the ultra-short chains to legacy long chain versions, all detected in a single injection, using mixed mode reverse phase and anion exchange separation column chemistries.

Key Learning Topics:

• Improved workflow for a wide range of PFAS compounds
• Mixed mode separations using a reverse phase and anion exchange column
• Improved analyte identification and data quality
• Addressing instrument contamination

Who should attend?

• PFAS Program Managers
• Laboratory Managers
• Scientists
• Quality Assurance Managers

For more information please contact [email protected] or [email protected]

Presenter: Kari Organtini, PhD (Principal Scientist, Waters Corporation)

Kari Organtini is a Principal Scientist at Waters Corporation and has been with the company for six years. She received her PhD from Penn State University in 2015 studying the occurrence of dioxins and furans in fire debris and their implications to first responder health. At Waters, Kari is part of the food and environmental applications team. During her career, Kari has developed applications using both LC and GC techniques for a variety of applications ranging from pesticides to persistent organic pollutants. She is currently the scientific lead for developing PFAS applications at Waters.