HPLC Method Development: From Beginner to Expert, Part 2

From beginner to expert, we can all benefit from a review of HPLC basics. In this two-part talk, we will work our way from basic chromatographic principles to advanced method development topics.

Method Development 102 will review and expand upon some of the 101 fundamentals as we cover advanced topics such as how to best transfer a method from one column dimension to another. We will explore reasons for why some methods are quite difficult to transfer to a different HPLC system. In addition, we will look at gradient method development and how to efficiently use a scouting gradient to quickly develop a good HPLC method.

Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)

Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.