Avoiding Chromatography Heartbreak - Common LC Pitfalls

Whether you’re new to HPLC or have been doing it for years, you may find that things just aren’t going the way you want them to.

There are many pitfalls or “gotchas” that can cause problems. Even the issue of what tubing and fittings are compatible and which are not can be a serious impediment to a successful HPLC separation.

In this talk, we will discuss:

• The importance of connections

• Mobile phase considerations

• Quality

• Preventing microbial growth

• Sample/solvent compatibility

• Column characteristics; their importance and limitations

• The importance of sample prep

Avoiding these pitfalls can help you achieve long-term, reliable results.

Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.