Getting Off to a Good Start: Isocratic Method Development

Isocratic methods for HPLC are quite popular due to their ease of use and transferability.

In this talk, we will look at different approaches to developing isocratic methods as well as the fundamentals of isocratic resolution. We will finish with a discussion of allowable adjustments to methods from reference compendiums and how these may make our jobs as method development chemists a bit easier.

Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)

Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.