Getting Off to a Good Start: Gradient Method Development
Gradient methods for HPLC are typically faster and more sensitive for many challenging separations.
In this talk, we will look at the differences between gradient and isocratic HPLC methods and how these differences benefit us. We will be looking at the step-wise process of method development, starting with a scouting gradient and ending with testing for ruggedness and robustness.
Whether you’re a basic user of gradient methods and only want a better understanding to help troubleshooting, or even if you are an experienced method developer, we will be discussing issues and concepts that should help us all improve our skill sets.
Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)
Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.
