Getting Off to a Good Start: Preparative Method Development Strategies
Agilent Technologies: Getting Off to a Good Start: Preparative Method Development Strategies
When developing an analytical HPLC method, our goal is the identification and quantitation of a compound of interest.
To achieve this goal we optimize parameters like baseline resolution, peak symmetry and peak width. But in preparative HPLC method development, our goal is the separation and isolation of a valuable compound. The analytical chromatographic parameters become less important—throughput, yield and purity of the compound are the more important parameters for preparative HPLC.
In this webinar, we will discuss the process of scaling up from an analytical to a preparative HPLC method. We will look at some key parameters and calculations as well as column and instrumentation considerations.
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.