Don’t Lose It: Getting Your Peaks in Shape
Decrease stress, increase performance… learn how to get good peak shape and what to do to when it goes away. Good peak shape is defined as a symmetrical or gaussian peak. It dramatically improves resolution, as well as accuracy and precision of quantitation. When you lose it, you may see things like peak fronting, tailing, shoulders, and split peaks.
In this presentation, we’ll look at how your column choice, your sample, and mobile phase can all affect your peak shape in both good and bad ways. Finally, we will discuss remedies to get the good peak shape back.
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
