Encore Performance!!! Thinking Outside the C18 Box
Agilent Technologies: Encore Performance!!! Thinking Outside the C18 Box
Back by popular demand!!! Reversed-phase with a C18 column is the typical first choice for many HPLC analyses. But what if it doesn’t work for your sample? For example: very polar compounds have poor retention with reversed-phase and enantiomers will not separate on a C18.
This talk will review other modes of chromatography, including NEW options available for HILIC and chiral separations.
We’ll also look at when normal phase, ion pair, ion exchange, or ion exclusion might be the best choice. Their strengths and weaknesses will be discussed as well as tips for developing methods with these techniques.
Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)
Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.