It Isn’t Always the Column: Troubleshooting Your HPLC Separation

You ran your HPLC and sample yesterday. Everything looked as expected. Today, the chromatography looks different, so it must be the column, correct? Not so fast. We can be quick to assume that the problem with our chromatography is with the HPLC column, but often there is another reason why the chromatography has changed.

In this seminar, we will discuss practical steps to identify the source of the problem and troubleshoot common issues. Considerations from sample preparation, mobile phase preparation, the column, to the HPLC system itself will be discussed.

Having an understanding for where problems can occur helps us quickly identify the problem, correct it, and prevent it from happening again.

Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)

Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.