Why Won’t My HPLC Method Transfer? How to Build a More Robust Method

Today’s analytical lab is busier than ever, regularly being asked to do more with less. In the name of efficiency, we are advised to “not reinvent the wheel” and simply transfer an existing method from a collaborative lab or a publication. Yet, how often do we attempt this and generate resulting data that is far different from our colleagues or the authors of the referenced article?

In this talk, we will discuss the factors that impact the fidelity of method transfer. We will look at how to measure and compensate for differences in these key system attributes.

Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)

Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.