Improving End-to-End Metabolite Identification by LC-MS/MS

Studies on drug metabolism aid in evaluating the pharmacokinetics, pharmacodynamics and safety during discovery and development. In vitro studies of drug metabolism are essential for identifying major metabolism pathways. These studies often use accurate mass spectrometry to ensure comprehensive profiling and identification of drug metabolites. However, confident identification of drug metabolites remains challenging due to insufficient fragment ion coverage.

Here, an alternative fragmentation technique is applied to produce additional fragment ions and an informative MS/MS spectra for metabolite identification. An end-to-end workflow is demonstrated for streamlined method development and metabolite identification from high-quality and more informative electron activated dissociation (EAD) MS/MS spectra.

Attend this webinar to learn how to:

• Perform confident drug metabolite identification using alternative fragmentation mechanism
• Harness the informative and rich data collection from the latest generation QTOF technology for sensitive drug metabolite identification
• Employ an end-to-end workflow for drug metabolite identification, from method development to data analysis

Presenter: Rahul Baghla (Global Pharma Applications Lead, SCIEX)