Quant Made Easy: Building a Calibration Curve with Agilent MassHunter Quantitative Analysis

• Wait what?! Who knew!? There are so many things that a mass spec can do.

• I've used it for years, yes this may be true.

• I've processed some samples, more than a few.

• But between me and you, I had no idea all the things it could do!

• From sample prep to separation, you just won't believe…

• All of the tips and tricks we have up our sleeve.

• So, take a few minutes, about 15 should do.

• And learn all the things your mass spec can do.

Building a calibration curve in a quantitative method is required for most LC/TQ studies. In MassHunter Quantitative software, the calibration curve basics will be reviewed and a convenient and quick method for importing the calibration levels from CSV file will be featured.

Presenter: Hui Zhao, PhD (LC/MS Applications Scientist, Agilent Technologies, Inc.)

Hui has 13 years of industry experience developing and validating analytical methods for food/feed nutrition, food safety, dietary supplements and botanicals testing, using a variety of analytical techniques including LC-TQ, LC-QTOF, LC-DAD and GC-MS and a breadth of sample preparation methodologies. She has worked as a Research Scientist at Monsanto, EPL-Bioanalytical Services, Tate & Lyle, Inc. and Lead Staff Scientist at Covance Food Solutions. She holds a Master of Science degree in Analytical Chemistry from Lanzhou University in China and a Ph.D. in Analytical Chemistry from the University of Missouri.

Prior to becoming a Pre-Sales AE, Hui worked as an LCMS application scientist in Agilent’s global market development group where her primary focus was food and environmental market development.

Presenter: Karen Yannell (LC/MS Application Engineer, Agilent Technologies, Inc.)

Karen E. Yannell is a LC/MS Application Engineer at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (QTOF) mass spectrometry for applied markets. Her recent work has focused on developing routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. Outside of the laboratory, she enjoys cooking unique dishes and hiking along the beach with her dog.