SEC Overview and Method Development for Biomolecules

Size exclusion chromatography (SEC) is a noninteractive technique that separates solutes according to their molecular size in non-denaturing conditions. It is often used as the first step in isolation of a protein from a crude sample.

We will discuss the theory behind SEC, overview of mAbs, and characterization requirements; with a focus specifically on the effect of solute size and molecular weight, general rules for using SEC, column selection choices, important mobile phase considerations, and column care. We will explore method development pointers in the troubleshooting section.

Presenter: Mohit Patel (Application Engineer, Agilent Technologies, Inc.)

Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.

Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.

Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.