Developing an Integrated High-Throughput Workflow for Microgram-Scale Purification

In recent years, successful miniaturization and automation of assays have reduced the scale of synthesis in drug discovery. This has enabled faster analysis, higher throughput, and reduced material consumption—lowering the cost per experiment without compromising data quality.

The purification of compounds of interest is a critical step in the library synthesis and purification process. It requires a robust, high-throughput, microgram-scale workflow.

This webinar demonstrates the development of such an end-to-end workflow. UHPLC/MS instrument optimization, column selection, column loading studies, and the benefits of ultrafast pre- and post-purification analysis will be highlighted.

Key Learning Objectives:

• How to optimize a microgram-scale UHPLC/MS purification instrument for high purity and recovery
• How to achieve higher throughput and faster turnaround times when purifying microgram-scale reactions

Who Should Attend:

• Laboratory managers
• Researchers
• Scientists

Presenter: Chandan Barhate (Principal Scientist, Synthesis and Analytical Technology Team, Bristol Myers Squibb)

Presenter: Ann Thayer (Contributing Editor, C&EN Media Group)