Accelerating Large-scale Proteomics with High-throughput Accurate Mass Spectrometry Workflows

Identifying and quantifying large numbers of proteins and peptides are important in translational research to understand biological functions. One example of this is the analysis of large sample cohorts to identify robust biomarkers of disease. The complexity of biological fluids, tissues and cell lines often overwhelms the capabilities of data-dependent acquisition (DDA) strategies using mass spectrometry due to the stochastic nature of this methodology. This limitation has driven the rapid adoption of data-independent acquisition (DIA or SWATH DIA) approaches, such as Zeno SWATH DIA. This method achieves 5-6x more MS/MS sensitivity, resulting in this DIA approach surpassing DDA for protein identifications and quantification in complex matrices. This advantage is further observed at short acquisition times that are required to meet the throughput demands of current proteomics studies.

This webinar will discuss high-throughput mass spectrometry workflows for large-scale qualitative and quantitative proteomics and will comprise the two presentations below.

Rapid and Robust Proteomes with Standardized Methods on the Evosep One Powered by Zeno SWATH DIA

Presenter: Dorte Bekker-Jensen (Senior Scientist, Evosep Biosystems)

Dorte works as a Senior Scientist at Evosep in the Application Development team. Evosep aims to make clinical proteomics 100 times more robust and 10 times faster by radically innovating sample preparation and separation prior to MS analysis.

Robust, High-throughput Quantification of Peptides in Plasma using Zeno SWATH DIA

Presenter: Patrick Pribil (Senior Manager, SCIEX Proteomics Applications Development)

Patrick Pribil completed his BSc in Biochemistry at McMaster University (Hamilton, Canada) in 1997, followed by his PhD in Biochemistry at The University of Western Ontario (London, Canada) in 2003. He spent 2 years as a Post-Doctoral Fellow at the University of Maryland, studying rapid identification of bacteria using MALDI-MS under Catherine Fenselau before joining SCIEX as an Applications Support Scientist in 2005. He has been an Applications Manager at SCIEX since 2013, managing teams supporting pharma, biopharma, proteomics, metabolomics and lipidomics applications before transitioning to the role of Senior Manager, Proteomics Applications Development in October 2022.

By attending this presentation you will learn about:

• innovations in accurate mass analysis delivering speed and accuracy for large-scale proteomics
• the development of a standardized platform for robust, in-depth high-throughput proteome analysis
• the impact of sensitivity gains on protein and peptide identification and quantification.

Who should attend:

Scientists and researchers utilizing proteomics workflows and/or working on the identification and quantification of large numbers of biomarkers/xenobiotics/NCEs across a biological population to understand their biological functions.