Analytical Strategies for Complete Characterization and Purification of Synthetic Oligonucleotides

Synthetic oligonucleotides have become the fast-growing therapeutic modality in recent years and are being increasingly developed for treating a wide range of disease conditions. Along with the development of these therapeutic candidates comes the increased need for reliable analytical methods and easy-to-use data analysis workflows to fully characterize them to ensure the intended efficacy. Thus, reliable and sensitive analytical and preparative methods are required to adequately resolve impurities and establish purity and identity of the intended oligonucleotide product.

In this presentation, we will present the relevant chromatography, mass spectrometry and spectroscopy based analytical strategies to confidently detect, quantify and purify oligonucleotides and their related impurities that can help you meet both the challenges of synthetic oligonucleotide quality assessment, and the productivity needs of your lab.

Learning Objectives

• Learn about the manufacturing challenges associated with synthetic oligo drugs
• Learn about the critical quality attributes for complete assessment of synthetic oligonucleotides
• Learn about the analytical approaches for the confident identification, quantification and purification of synthetic oligonucleotides and the related impurities

Presenter: Parul Angrish, PhD (Director, Biopharma/Pharma Market, Agilent Technologies, Inc.)

Parul Angrish is a Director, Biopharma/Pharma Market at Agilent Technologies. Her focus includes advancing Agilent’s strategic solutions to meet biopharma and pharma customer needs. She has over 15 years of extensive experience in innovating, commercializing and leading cross-functional teams to drive business growth opportunities. Prior to joining Agilent Technologies, she had held several positions at Thermo Fisher Scientific, Life Technologies and DiscoveRx Corporation. She received her Ph.D. in chemistry from the University of Florida. She has co-authored several peer-reviewed articles and is an inventor on several patents.