Ultra-High Sensitivity for Targeted Proteomic using Evosep-MRM at Single Cell Level

The Evosep One is a powerful and standardized platform for high sensitivity work including single cell proteomics. The robustness and throughput of the Whisper methods on the Evosep One combined with the sensitivity of the Agilent 6495C triple quadrupole support targeted analysis of proteomes at the single cell level.

We demonstrated this by targeting selected peptides in a complex background of sub nanogram material combined with dilution curves spanning several orders of magnitude. To ensure seamless integration of the Evosep One with the Agilent 6495C triple quadrupole, we have developed a native driver for MassHunter and a robust solution for the Agilent nanoflow ion source.

Presenter: Nicolai Bache, Ph.D. (Co-Founder, Evosep Biosystems)

Nicolai Bache has a PhD in mass spectrometry and proteomics from the University of Southern Denmark followed by a two-year postdoc at the Children’s Medical Research Institute in Sydney. He then worked for several companies such as Proxeon Biosystems, Thermo Scientific and BrukerDaltonics before co-founding Evosep Biosystems in 2016 with a clear mission to enable and promote clinical proteomics. At Evosep, Nicolai is head of applications and leads the technology and application development together with leading experts in the field.