Comprehensive, Automated, and Integrated Software for Oligonucleotide Characterization and Sequence Confirmation

Oligonucleotides including small interfering RNA, antisense oligonucleotides, aptamers, and CRISPR guides have become fast growing modalities in recent years. Along with the development of these candidates has come the increased need for robust analytical methods and easy to use data analysis workflows to characterize them. Critical quality attributes of oligo samples include the determination of the target, confirmation of its sequence, and identification of impurities present each of which can be time consuming, difficult, and tedious. As such, software that supports and automates these efforts can be of great value.

In this work, we present novel, automated, and integrated software to support these workflows using HRAM MS data.

Presenter: Peter Rye, Ph.D. (LC/MS Applications Scientist, Agilent Technologies, Inc.)

Presenter: Peter is a pre-sales Application Engineer with Agilent Technologies. He has been with Agilent for 10 years developing LC, RapidFire, and mass spec methods on a wide range of molecules. Prior to Agilent, Peter worked in analytical development groups for BioTove and BIOCIUS, the original companies that developed the RapidFire platform. In his spare time, you can find Peter relaxing with his wife and kids in Beverly Massachusetts, trying to fix the many problems with his home, or staying active outdoors.