Imaging Lipids in a Brain Injury model with AP-MALDI on Agilent Tandem Quadrupole Mass Spectrometers

Traumatic brain injury (TBI) is a neurological condition that can lead to cognitive decline, physical and psychological impairments. The use of a controlled cortical impact (CCI) mouse model can effectively replicate TBI and the localized nature of the injury provides an ideal platform for visualizing in situ molecules affected by the injury, e.g., lipids within brain tissue.

Atmospheric Pressure Matrix-Assisted Laser Desorption/ionization (AP-MALDI) mass spectrometry imaging (MSI) allows the study of localized, in situ molecules resulting from brain injury.

In this study, we highlight AP-MALDI imaging with a high sensitivity tandem quadrupole (TQ) and our data highlights the compatibility of using AP-MALDI on Agilent mass spectrometers to selectively detect and image endogenous mouse brain lipids.

Presenter: Xi Qiu, PhD (LC-MS Application Scientist, Agilent Technologies, Inc.)

Xi Qiu is application scientist at Agilent, he earned his Ph.D. degree from the department of Pharmacognosy and Medicinal Chemistry at University of Illinois at Chicago. Prior to joining Agilent, he worked at Johnson and Johnson where he focused on large molecule quantification using LC-MS at intact and peptide level as well as contract research organizations leading large molecule LC-MS group performing protein, protein conjugates, peptide, and oligos analysis.