Dual deconvolution of LC-PDA-MS data: A fast-track approach for chemical composition and impurity analysis

Now easily integratable into liquid chromatograph systems, mass spectrometry (MS) is increasingly recognized as an orthogonal detector that adds extra information to UV-based photodiode array (PDA) detectors. Analysis of chemical compositions and/or impurities is a typical application employing both PDA and MS detectors for enriching the information obtained from a single analysis.

One barrier to the adoption of MS detectors has been software to simplify the interpretation of PDA and MS data. Shimadzu’s compact LC-PDA-MS system operates on LabSolutions software, which is now equipped with deconvolution algorithms for both PDA and MS data.

This webinar will reveal a streamlined workflow based on the dual deconvolution for resolving complexities caused by insufficient chromatographic separations. The approach is suited for both routine and research laboratories seeking to reduce time from samples to results.

Key learning objectives

• Familiarize with Shimadzu’s new compact MS detector, LCMS-2050.
• Learn how MS data helps spot co-eluting components and UV-transparent peaks.
• Mass-it™ is a sophisticated algorithm that unravels complex MS data and labels key outcomes onto a UV chromatogram.
• i-PDeA-II™ is a deconvolution algorithm for PDA that can resolve co-eluting impurities

Who should attend?

• Managers, supervisors and senior lab technicians in research laboratories seeking to maximize the outcome of LC-PDA-MS analysis.
• Lab managers in routine laboratories looking to reduce data analysis time.

Presenter: Dr. Atsuhiko (Ash) Toyama (Global Product Manager (LCMS), Shimadzu Corporation)

Ash Toyama has 10 years of proteomics research experience. His area of expertise also includes quantitative mass spectrometry with a particular focus on bioanalysis, metabolomics and endocrinology. At Shimadzu Corporation, he has been involved in academic research collaborations, application development, and in the specification development of mass spectrometers. Toyama holds a Master of Science degree in Biochemistry from the University of Cambridge, UK, and a Ph.D. degree in Biological Sciences from the University of Tokyo for his research on clinical proteomics.

Presenter: Dr. Davide Vecchietti (Global Product Manager (LC), Shimadzu Corporation)

Davide Vecchietti has 10 years of Biochemistry and Microbiology research experience. His areas of expertise include the development of analytical methods for biological molecules characterization, proteomics methods for the study of pathogen bacteria, and quantitative mass spectrometry with a focus on bioanalysis. At Shimadzu Corporation, he has been involved in application development of LCMS methods and in the specification development of Liquid Chromatography products. Vecchietti holds a Master of Science degree in Molecular Biology from the University of Milan, Italy, and a Ph.D.degree in Biochemistry from the University of Milan for his research on low - molecular - weight heparins characterization.

Presenter: Ellen Simms (SelectScience)

Ellen studied biological sciences at the University of the West of England, UK, before completing a master's in epidemiology from the University of Bristol. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.