Chromatographic Separation of Biological Macromolecules

Liquid chromatography is divided into categories based on the mechanism of interaction of the analyte with the stationary phase, which leads to separations created through the electrical charge, molecular size, or polarity of the solute. One such kind of solute mixture is proteins and biomolecules, which can be very complex in nature and thus, challenging to isolate and purify.

In this webinar, we will explore the various chromatography techniques used for proteins and peptides, including affinity chromatography (AC), hydrophobic interaction chromatography (HIC), ion exchange chromatography (IEX), reverse phase chromatography (RPC), and size exclusion chromatography (SEC). Additionally, we will discuss parameters that effect separation due to electric charge, polarity, or molecular size.

Presenter: Mohit Patel (Application Engineer, Agilent Technologies, Inc.)

Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.

Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.

Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.