Analysis of Glyphosate, glufosinate and AMPA in Environmental Water with direct injection
Monitoring highly polar, small organic pesticides such as glyphosate, AMPA and glufosinate in water as well as in food presents an analytical challenge.
The difficulties to determine these compounds at trace levels are associated with their physicochemical properties affecting their solubility, retention and sensitivity. Therefore, a FMOC-derivatization workaround is widely used and well established to overcome the analytical challenges, but with the evolution of column chemistry and excelled tandem quad sensitivity, there is an alternative LC-MS/MS approach possible that allows the direct analysis of the highly polar anionic compounds.
Glyphosate, AMPA and glufosinate retain on a specialized mixed mode column even when injecting up to 50 µl water sample directly. During last year’s Blue Thursdays webinar series, we presented results on the dedicated Waters Anionic Polar Pesticide Column (APPC) to achieve robust data at 30 ng/L in drinking water samples.
As Waters have recently launched a new high-end tandem-quad mass spectrometer - the Xevo TQ Absolute - it is time to check how this new technology can take us down to even lower levels while maintaining robustness. On this journey, we would also like to expand our matrix coverage from drinking water to more challenging environmental waters.
In the upcoming webinar Benjamin Wuyts will present the results of a method combining stable retention on APPC, ultra-performance chromatography on the ACQUITY I-Class and absolute MS power with the Xevo TQ Absolute to provide a fast and robust solution to one of the most challenging and time-consuming applications in environmental analysis.
Presenter: Benjamin Wuyts (Senior Solution Support Specialist, Waters Corporation)
Benjamin initially joined Waters as an application chemist working in the Belgian demo lab, performing liquid chromatography-mass spectrometry demonstrations, support and training. In 2016 Benjamin became a European application chemist focusing on projects to support emerging applications. Benjamin has been focused over several years in establishing the Waters solution to the analysis of polar anionic pesticides, utilising novel column phases and LC-MS/MS technologies.
