Strategies to Enable and Simplify HPLC Polar Compound Separation

When it comes to the HPLC analysis of polar water-soluble compounds by reversed-phase chromatography, things can get complicated and method simplification and reproducibility becomes even more important.

Polar water-soluble compounds are generally difficult to retain on hydrocarbon based (n-octyl or octadecyl) stationary phases and the separation of a mixture of water-soluble compounds on such types of columns can be challenging.

Many of us know or use methods that utilize ion-pairing mobile phase techniques to enable the retention and separation of polar analytes. However, many ion-pairing mobile phases are complicated and difficult to control.

PhD Matthew Przybyciel, R&D Leader at PerkinElmer will present how, even the most difficult analyses, can be simplified and demonstrate how the latest in column developments can enable easier and faster HPLC analysis of polar compounds for an end-to-end workflow solution that can improve your lab productivity.

In This Exciting Live Webinar You'll Learn About:

• Ways to tackle the most difficult HPLC polar compund analyses
• Which columns are best suited for polar compounds
• Simplifying methods and avoiding the use of ion-pairing mobile phases
• Examples for a variety of food and pharma applications that require the retention and separation of polar compounds

Who Should Attend:

Lab managers or directors, pharma QA/QC employees & management, food and beverage safety and quality employees & management, chromatography chemists, lab scientists, university professors, preparative chromatography specialists.

Presenter: Matthew Przybyciel (R&D Leader, PerkinElmer)

Matthew has a PhD in Analytical Chemistry from the University of South Carolina. The focus of his research was the development of new stationary phases for capillary gas chromatography. In the late 1990’s he worked in ES Industries focusing its efforts exclusively on chromatography column products for HPLC and SFC where Matt was Vice President and Technical Director for chromatography products. He has successfully developed and commercialized a number chromatographic stationary phases and columns for high performance chromatographic separations for HPLC and SFC.

In 2021, ES Industries became part of PerkinElmer, Inc. and Matthew now is product development leader at PerkinElmer.

As part of his work, he has also published a number of articles on the development and application of new stationary phases for GC, HPLC and SFC.