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Super-charge your method development with a quick, easy, universally compatible LC and LC/MS method

ZÁZNAM | Proběhlo St, 2.3.2022
Scott Bollen, demonstruje, jak můžete dosáhnout lepšího výkonu pro základní analyty za podmínek slabé iontové síly v mobilní fázi, jako je kyselina mravenčí a také ukáže nové nástroje pro vývoj metod.
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SelectScience: Super-charge your method development with a quick, easy, universally compatible LC and LC/MS method

SelectScience: Super-charge your method development with a quick, easy, universally compatible LC and LC/MS method

LC and LC/MS method developers across industries need to create fast, reproducible, and easily transferable methods. Formic acid is an ideal mobile phase modifier, creating quick, easy, and highly reproducible conditions for LC analyses and allowing highly transferable methods across LC detectors. This includes its exceptional compatibility and improved sensitivity with LC/MS detection. However, traditional LC columns have struggled to produce results with formic acid for basic compounds, resulting in additional method development and often the use of less desirable mobile phase additives.

In this webinar, Scott Bollen, business development manager EMEA at Agilent, demonstrates how you can achieve enhanced performance for basic analytes under weak ionic strength mobile phase conditions, such as formic acid. Scott also outlines a new method development tool: a unique C18 bonded phase chemistry with a charged surface on 2.7 µm superficially porous particles.

This new stationary phase, applied to 2.7 µm superficially porous particles, creates a remarkably flexible LC column that can be used across many instruments and detector platforms with a simple formic acid mobile phase, allowing effortless transfer between laboratories with varying instrumentation.

Key Learning Objectives:

  • Create a flexible and universally compatible LC and LC/MS method using a novel superficially porous particle column with a simple formic acid mobile phase.
  • The pros and cons of using formic acid, as well as tips and tricks to ensure a robust method with excellent performance, especially for basic analytes.
  • How to save time and money when scaling and transferring your new universal method, by avoiding additional method development due to different instrument requirements.

Who Should Attend:

Anyone in a laboratory wanting to learn more regarding new column orthogonality and method development.

Presenter: Scott Bollen (Business Development Manager EMEA, Agilent)

Scott specializes in small molecule LC columns, ranging from HPLC to UHPLC. Scott has worked at Agilent for 17 years. He started as a technical support manager, and now supports new LC products in R&D as well as the entirety of the existing small molecule LC column portfolio in business development.

Presenter: Blake Forman (Editorial Team, SelectScience)

Blake studied chemistry at the University of Surrey, UK, and further went on to study a master’s in chemistry at the University of Southampton, UK. Blake has previously worked in the Medicines and Healthcare Products Regulatory Agency (MHRA) lab at LGC and as a technical sales specialist for Waters. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.

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