Native MS for intact protein analysis

The challenge of studying intact proteins and protein complexes in their biological state is one that has been met in recent years by the development of native mass spectrometry. The technique is key to understanding structural biology by enabling the proteomics researcher to characterise protein complex assemblies and determine the degree and pattern of post translational modifications (PTMs) on individual proteins.

Spatially resolved native mass spectrometry analysis of protein complexes using nano-DESI

Presenter: Oliver Hale (School of Biosciences, College of Life and Environmental Sciences, Birmingham, UK)

Dr Oliver J. Hale was awarded his PhD in Chemistry in November 2018 from the University of Reading, Reading, UK. He has held a postdoctoral research fellow position in the lab of Professor Helen J. Cooper at the University of Birmingham since April 2019. His work involves the development of techniques and methods for in situ analysis of intact protein assemblies including mass spectrometry imaging, top-down proteomics, and gas phase separation.

Native mass spectrometry enables elucidation

Presenter: Tarick El-Baba (Kavli Institute for NanoScience Discovery and Physical and Theoretical Chemistry Laboratory, University of Oxford)

Tarick earned his B.S. in Chemistry from Wayne State University (Detroit, MI, USA) and completed his Ph.D. in Chemistry under the tutelage of Prof. David Clemmer at Indiana University. There, he focused on developing mass spectrometry to study in solution protein biophysics from biological mixtures. Tarick is currently a Senior Researcher and co-principal investigator in Prof. Dame Carol Robinson’s laboratory at the Kavli Institute for NanoScience Discovery at the University of Oxford. He is focused on developing native mass spectrometry to understand biological mechanisms that underly neuropsychiatric diseases.

Connecting Proteoform Diversity to Receptor Function using Native Top-down Proteomics of G Protein-Coupled Receptors on the Thermo Scientific Orbitrap Eclipse

Presenter: Corinne Lutomski (Kavli Institute for NanoScience Discovery and Physical and Theoretical Chemistry Laboratory, University of Oxford)

Corinne earned a B.S. in Chemistry from Wayne State University (USA) and a Ph.D. in Analytical Chemistry from Indiana University (USA). Corinne is now a Senior Researcher and Co-Principal Investigator at the Kavli Institute for Nanoscience Discovery at the University of Oxford. She is an expert in biological mass spectrometry and is best known for developing methods in mass spectrometry for interrogating large protein complexes. Her research continues to focus on instrument development to enable native biological mass spectrometry with particular focus on membrane protein receptors within the brain.

TBC

Presenter: Oscar Hernandez

Learning points:

• Latest advances in proteomics data analysis
• Latest advances in proteomics mass spectrometry
• Optimising strategies to maximise research performance with confidence

Who should attend:

• Researchers in proteomics labs
• Researchers in structural biology labs
• Laboratory directors, managers and technicians interested in state-of-the-art protein analysis