HPLC Mobile Phase Optimization

Key Learning Objectives

• Understand how different reverse-phase organic solvents change selectivity of common stationary phases
• Understand the role of pH and ionic strength in reverse-phase LC
• Learn when to use isocratic and gradient methods, and how to optimize gradient methods for efficient separations

Who Should Attend

Reverse-phase LC method developers, and anyone involved in HPLC who would like a greater understanding of the role of mobile phase in reverse-phase LC

Presenter: Dr. Aidan Harrison (Sr Field Applications Specialist, Phenomenex)