An Introduction to HPLC/ UHPLC Method Scaling

ZÁZNAM | Proběhlo Čt, 28.10.2021

Budeme diskutovat o správném škálování metody, o vlivu velikosti částic na účinnost, o průtoku pro daný vnitřní průměr, o zlepšení účinnosti a o změně měřítka směrem k preparativním purifikacím.

Přejít na webinář

Phenomenex: An Introduction to HPLC/ UHPLC Method Scaling

Key Learning Points:

Benefits of Proper Method Scaling during method development
The Effect of Particle Size vs. Efficiency
Adjusting Flow Rate for the Intended Internal Diameter
Optimization of Particle Size, Flow Rate, Column Dimensions
Alternatives for Improving Efficiency within Instrument Limitations
How to Apply Scaling Towards Preparative Purifications

Presenter: James Turner (Technical Manager, Phenomenex Europe)

With more than 16 years of experience at Phenomenex, James has been responsible for providing specialist technical support to customers across Europe with method development assistance, troubleshooting advice and chromatographic solutions to various customer problems. James has also been involved with the technical training of many Phenomenex technical consultants, across the multiple techniques we serve.

Phenomenex

An Introduction to HPLC/ UHPLC Method Scaling

Key Learning Points:

Mohlo by Vás zajímat

Simultaneous Quantitation and Discovery analysis: Combining targeted and untargeted metabolomics on Orbitrap mass spectrometers

Analysis of Per- and Polyfluoroalkyl Substances (PFAS) in Wastewater

Non-Targeted Screening of Biosolids with the Xevo™ MRT Mass Spectrometer Reveals New Isoforms of PFAS

High Resolution Characterization of Lipid Nanoparticles Using the Xevo™ Charge Detection Mass Spectrometry (CDMS) Instrument - Single Particle Mass Analysis of Intact LNP-mRNA Formulations

Out-of-the-box workflow for PFAS quantitation using a fullscan high-resolution approach with the Orbitrap Exploris EFOX Mass Detector