An Introduction to HPLC/ UHPLC Method Scaling

Key Learning Points:

• Benefits of Proper Method Scaling during method development
• The Effect of Particle Size vs. Efficiency
• Adjusting Flow Rate for the Intended Internal Diameter
• Optimization of Particle Size, Flow Rate, Column Dimensions
• Alternatives for Improving Efficiency within Instrument Limitations
• How to Apply Scaling Towards Preparative Purifications

Presenter: James Turner (Technical Manager, Phenomenex Europe)

With more than 16 years of experience at Phenomenex, James has been responsible for providing specialist technical support to customers across Europe with method development assistance, troubleshooting advice and chromatographic solutions to various customer problems. James has also been involved with the technical training of many Phenomenex technical consultants, across the multiple techniques we serve.