High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients
Aplikace | 2020 | BrukerInstrumentace
Iontová mobilita, LC/TOF, LC/HRMS, LC/MS, LC/MS/MS
ZaměřeníProteomika
VýrobceBruker
Klíčová slovapasef, dia, evosep, timstof, resource, spd, mobility, spectronaut, data, bruker, precursor, library, specific, tims, protein, ion, gradients, throughput, one, libraries, using, pro, were, runs, hela, proteomic, reproducibly, daltonics, peptide, reproducible, short, dda, engine, acquired, covered, conceptionally, precursors, identified, project, sensitivity, new, per, sequences, combination, trifluoroethanol, turn, windows, dimension, isolation, pulsar
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High throughput proteomics Application of dia PASEF and Evosep One for short gradients
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dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…
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diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…
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