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High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients

 

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High throughput proteomics – Application of dia-PASEF and Evosep One for short gradients ASMS 2020, MP122 Stephanie Kaspar-Schoenefeld1, Markus Lubeck1, Thomas Kosinski1, Scarlet Koch1, Oliver Raether1, Gary Kruppa1 1Bruker Daltonik GmbH, 28359 Bremen, Germany Introduction Data-independent acquisition (DIA) promises reproducible…
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pasef, pasefdia, diaevosep, evoseplibrary, librarylibraries, librariestimstof, timstofhela, helausing, usingdata, datashort, shortmuntel, muntelresourcespecific, resourcespecificresource, resourcegradients, gradientsproteomics
dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…
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pasef, pasefdia, diahela, helayeast, yeastmobility, mobilitycumulative, cumulativeprotein, proteinlibrary, librarycoverage, coveragepeptides, peptidesspectronaut, spectronautwindows, windowsion, iontims, timsgroups
Ultra-high sensitivity proteomics on the timsTOF SCP The sensitivity of the mass spectrometer plays a crucial role in analyzing low input samples and single cell proteomics. Abstract The timsTOF Pro platform, introduced in 2017, already featured a sensitivity boost from…
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scp, scptimstof, timstofevosep, evoseppeptide, peptideion, ionwhisper, whisperloads, loadsmimicking, mimickingcell, cellbeam, beamresulting, resultingproteomics, proteomicsnanogram, nanogramsingle, singleoptic
diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…
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hye, hyediapasef, diapasefspectronaut, spectronautdia, diatimstof, timstofpasef, pasefproteomics, proteomicstriply, triplywindows, windowsproteomes, proteomesapproaches, approachesprotein, proteinpro, proion, ionquantification
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