Pharmacokinetic Analysis Using iMScopeTM QT During Corn and Soybean Seed Treatment with Pesticides

Význam tématu

Pesticidni osetreni semen predstavuje klicovou technologii pro zvyseni ucinne ochrany plodin v ranem vyvojovem stadiu a soucasne umoznuje snizeni davky fungicidu pri dalsich osetrenich. Spatialni a casove zobrazeni distribuce pesticidnich slozek v semennych tkanych prispiva k optimalizaci formulaci a minimalizaci ekologicke zateze. Imaging masove spektrometrie MALDI MSI poskytuje label free pristup bez potreby radioaktivniho oznaceni a umoznuje detailni mapovani chemicke lokalizace.

Cile a prehled studie

Cilem studie bylo vyuziti masove spektrometrickeho zobrazovani pomoci pristroje iMScope QT k lokalizaci fungicidu ethaboxamu v povrchove upravenych semenech kukurice a soji pred vysevem a padesat hodin po vysevu. Studie srovnavala distribucni vzory pestici­du v ruznych semennych strukturach a sledovala casove dynamicke zmeny.

Pouzita metodika

Priprava vzorku a analyza zahrnovala:

• Osetreni semen ethaboxamem ve dvou davkach (soja 75 g a.i./100 kg, kukurice 37,5 g a.i./100 kg)
• Flash freezing a kriosekaci semen na rezich tloustky 15 mikrometru
• Depozici matice alpha CHCA na ITO sklo pomoci iMLayer pri kontrolovane teplote a tloustce
• MALDI MSI analyzu na iMScope QT v pozitivnim modu m/z 300 330 pro MS a 100 330 pro MSMS
• Rekonstrukci obrazov a extrakci intenzit s pouzitim software IMAGEREVEAL MS

Pouzita instrumentace

• Matrix depositor iMLayerTM
• Imaging mass spectrometer iMScopeTM QT
• Data analysis software IMAGEREVEALTM MS

Hlavni vysledky a diskuse

• Pred vysevem oboji semena vykazovala silnou akumulaci ethaboxamu na povrchu
• U kukurice byla patrna penetrace do endospermu a hromadeni v embryu
• U soji ethaboxam zustal predevsim na povrchu bez vyrazneho vnitrniho prunik
• Po vysevu se u soji intenzita povrchoveho signalu rychle snizila a migrace do vnitrni casti byla minimalni
• U kukurice zustalo povrchove nahromadeni zachovano a soucasne se vyrazne zvysila intenzita v embryu
• Rozdily v distribuci a dynamice pestici­du jsou spojene s odlisnou semennou strukturou a interakci s pudou

Prinose a prakticke vyuziti

MALDI MSI metodika poskytuje bezlabelovy a prostorove detailni pristup k vizualizaci lokalizace pesticidu v semennych tkanych. Umí casove sledovat prenos cinnych latky, podporuje vyvoj optimalnich formulaci semennych osetreni a prispiva k presnejsimu rideni davkovani pesticidu s nizsi ekologickou zatezi.

Budouci trendy a moznosti vyuziti

• Rozsireni aplikace na dalsi druhy plodin a agrochemicke analyty
• Integrace casovych radu pro dynamickou vizualizaci pod realnymi podminkami
• Multimodalni kombinace s metabolomikou a molekularnim zobrazovanim
• Vyvoj novych matric a zlepsena spatialni rozliseni pro sledovani mikrodistribuce pesticidu

Zaver

MALDI MSI na pristroji iMScope QT umoznil jedinecne zobrazeni distribuce fungicidu ethaboxamu v semennych tkanych kukurice a soji pred a po vysevu. Poznatek o rozdilnem pruchodu chemicke latky v zavislosti na semenne strukture muze byt vyuzit k vylepseni formulaci semennych osetreni a snizeni pesticidni zateze v polnich podminkach.

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