<p><strong>Tento návod vysvětluje výběr vhodné kolony pro size exclusion chromatography (SEC) při analýze biologických molekul. SEC separuje molekuly na základě jejich hydrodynamického poloměru, tedy podle velikosti a tvaru, nikoliv podle molekulové hmotnosti.</strong></p><ul><li>Molekuly pronikají do pórů stacionární fáze</li><li>Nedochází k chemickým interakcím</li><li>Větší molekuly eluují rychleji</li><li>Menší molekuly eluují později</li></ul><h3><span><strong>Princip separace v SEC</strong></span></h3><ul><li><strong>Velké molekuly</strong><br>→ nevstupují do pórů<br>→ pohybují se pouze mezi částicemi (intersticiální prostor)<br>→ eluují dříve</li><li><strong>Malé molekuly</strong><br>→ vstupují do pórů<br>→ mají delší dráhu<br>→ eluují později</li></ul><h3><span><strong>Klíčové parametry pro výběr kolony</strong></span></h3><h4><span><strong>1. Charakter molekuly</strong></span></h4><p><strong>Zohledňuje se:</strong></p><ul><li>hydrodynamický poloměr</li><li>tvar molekuly</li></ul><p><strong>Příklady:</strong></p><ul><li><strong>peptidy: </strong>~0,7 nm</li><li><strong>AAV: </strong>~25 nm</li><li><strong>mRNA: </strong>protáhlý, neglobulární tvar</li></ul><h4><span><strong>2. Velikost pórů</strong></span></h4><ul><li>Optimální velikost:<br><strong>3–4× větší než poloměr analyzované molekuly</strong></li></ul><h4><span><strong>3. Velikost částic</strong></span></h4><ul><li>≤ 3 µm</li><li>zajišťuje vysokou účinnost separace</li></ul><h4><span><strong>4. Materiál stacionární fáze</strong></span></h4><ul><li>Preferována <strong>mechanicky odolná silika</strong></li><li>Odolnost vůči zpětnému tlaku</li></ul><h4><span><strong>5. Povrchová chemie</strong></span></h4><ul><li><strong>Chemická inertnost povrchu je zásadní</strong></li><li>Preferována je <strong>diolová chemie</strong>, protože:<ul><li>minimalizuje interakce s analytem</li><li>poskytuje <strong>vysoce hydrofilní povrch</strong></li></ul></li></ul><h4><span><strong>6. Konstrukce kolony</strong></span></h4><ul><li><strong>bioinertní provedení</strong></li><li>zajišťuje:<ul><li>minimální ztráty analytu</li><li>vysokou reprodukovatelnost</li></ul></li></ul><h3><span><strong>Dostupné SEC kolony (</strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=353&amp;manufacturerItems=50&amp;search=dsec"><span><strong>řada BioZen dSEC</strong></span></a><span><strong>)</strong></span></h3><h4><span><strong>1. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/2192"><span><strong>90 Å (9 nm)</strong></span></a></h4><ul><li>peptidy</li><li>malé proteiny</li><li>agregáty do ~50 kDa</li></ul><h4><span><strong>2. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/1534"><span><strong>200 Å</strong></span></a></h4><ul><li>protilátky</li><li>konjugáty protilátka-léčivo</li><li>krátké oligonukleotidy</li></ul><h4><span><strong>3. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/1535"><span><strong>700 Å (70 nm)</strong></span></a></h4><ul><li>AAV</li><li>mRNA</li><li>velké proteinové komplexy (např. IgM)</li></ul><h3><span><strong>Charakteristiky kolon</strong></span></h3><ul><li>siliková matrice</li><li>diolová chemie (hydrofilní, inertní)</li><li>bioinertní hardware</li><li>vysoká výtěžnost a reprodukovatelnost</li></ul><h3><span><strong>Výkon a kalibrace</strong></span></h3><h4><span><strong>Proteiny a AAV</strong></span></h4><ul><li><strong>90 Å</strong> → nízké molekulové hmotnosti (≤ 50 kDa)</li><li><strong>200 Å</strong> → střední rozsah (do ~450–500 kDa)</li><li><strong>700 Å</strong> → velké molekuly (včetně AAV)</li></ul><h4><span><strong>RNA a DNA</strong></span></h4><ul><li>separace molekul s neglobulárním tvarem</li><li><strong>200 Å</strong> → menší oligonukleotidy</li><li><strong>700 Å</strong> → dlouhé řetězce (až ~3000–4000 nukleotidů)</li></ul><h3><span><strong>Závěr</strong></span></h3><p><strong>Správný výběr SEC kolony vyžaduje:</strong></p><ul><li>znalost velikosti a tvaru molekuly</li><li>odpovídající velikost pórů</li><li>vhodnou velikost částic</li><li>inertní chemii a konstrukci</li></ul><p>To umožňuje dosáhnout optimální separace biologických molekul.</p>

Jak vybrat správnou SEC kolonu pro analýzu biologických molekul

<p><strong>Zveme vás na odborný seminář zaměřený na moderní svět separačních technik a hmotnostní spektrometrie. Seminář se bude konat 29. dubna 2026 od 13:00 hodin v Aule Arnošta z Pardubic (Studentská 519, Pardubice).</strong></p><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://forms.office.com/Pages/ResponsePage.aspx?id=aR-wpy9pT0iMxW5H7RJK8WO2jiQLb7NOszjVkgYyezJUMFNTVktGUUZCTUIzMUwzMUM5TDM5QTFLUS4u"><strong>Registrace</strong></a></p><p><i>Kapacita je omezená, žádáme Vás o registraci</i></p><p><i><strong>Občerstvení bude zajištěno.</strong></i></p></blockquote><h3><strong>Program</strong></h3><h5><strong>13:00 Vítejte!</strong></h5><ul><li>Lukáš Plaček</li></ul><h5><strong>Nová generace HPLC&nbsp;</strong></h5><ul><li>Zuzana Snížková</li></ul><h5><strong>Plynová a iontová chromatografie v aplikacích&nbsp;</strong></h5><ul><li>Magdalena Voldřichová</li></ul><h5><strong>Síla LC-MS s jednotkovým rozlišením&nbsp;</strong></h5><ul><li>František Zaleš</li></ul><h5><strong>Potřeba vysokého rozlišení v LC-MS</strong></h5><ul><li>Roman Hájek</li></ul><h5><strong>14:15 Diskuze a zakončení</strong></h5><p>&nbsp;</p><p><strong>Těšíme se na Vás.</strong></p><p><strong>Váš tým Pragolab s.r.o.</strong></p>

Pozvánka na Pragolab seminář Moderní svět separačních technik a hmotnostní spektrometrie

<p><strong>HALO® BIOCLASS Oligonucleotide kolony posouvají analýzu oligonukleotidů na novou úroveň. Využívají osvědčenou Fused-Core® technologii společnosti AMT, která zajišťuje vysoce efektivní a rychlé separace optimalizované přímo pro potřeby oligo analýzy. Nejnovější přírůstek do portfolia – kolona OLIGO 1000Å – přináší ještě lepší výkon při separaci větších a strukturálně složitějších oligonukleotidů.</strong></p><p>Díky tomu HALO® nabízí kompletní OLIGO řešení pokrývající široké spektrum separačních výzev.</p><ul><li><strong>P2762-102: </strong>HALO 1000 Å OLIGO C18 , 2.7 µm, 2.1 x 5 mm, 3 pk.</li><li><strong>P2762-302: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 30 mm - INERT</li><li><strong>P2762-402: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 50 mm - INERT</li><li><strong>P2762-602: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 100 mm - INERT</li><li><strong>P2762-702: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 150 mm - INERT</li><li><strong>P2762-902:</strong> HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 250 mm - INERT</li><li><strong>P2A62-402:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 2.1 x 50 mm</li><li><strong>P2A62-602:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 2.1 x 100 mm</li><li><strong>P2A62-702: </strong>HALO 120 Å OLIGO C18, 2.7 µm, 2.1 x 150 mm</li><li><strong>P2A64-402: </strong>HALO 120 Å OLIGO C18, 2.7 µm, 4.6 x 50 mm</li><li><strong>P2A64-602:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 4.6 x 100 mm</li><li><strong>P2A64-702:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 4.6 x 150 mm</li></ul><blockquote><p>Více informací najdete na stránce <a target="_blank" rel="noopener noreferrer" href="https://halocolumns.com/solutions/biopharma-solutions/oligonucleotide-solutions/"><strong>OLIGO Solutions | HALO®</strong></a>.</p></blockquote><h4><strong>Kolony HALO® BIOCLASS OLIGO NABÍZEJÍ:</strong></h4><ul><li><strong>Velikost pórů 120 Å</strong> – vhodné pro oligomery až do délky cca 60 bází</li><li><strong>Velikost pórů 1000 Å</strong> – pro vysoce účinnou separaci oligomerů o délce 100 a více bází</li><li><strong>Vysokou stabilitu při vysokém pH a zvýšené teplotě</strong></li><li><strong>Stacionární fázi kompatibilní s UHPLC a hmotnostní spektrometrií</strong></li><li><strong>Hardwarově pasivovaný povrch kolony</strong>, který snižuje adsorpci oligonukleotidů</li></ul><h4><strong>Brožury</strong></h4><ul><li><strong>Kolony HALO Oligo C18</strong></li><li><strong>Kolony HALO 1000 Å OLIGO C18</strong></li></ul>

HALO® BIOCLASS Oligonucleotide kolony

Akademie

Video

Článek

Produkt

Nejbližší akce

Je autorizovaným distributorem společnosti Agilent Technologies pro Českou republiku v oblastech chromatografie (GC, HPLC, UHPLC, kapilární elektroforéza) a hmotnostní spektrometrie (GC/MS, LC/MS, CE/MS), disolučních aparátů, molekulární (UV-Vis, UV-Vis-NIR, fluorescence, FTIR analyzátory a mikroskopy) a atomové spektroskopie (ICP-OES, ICP-MS, AAS), a v neposlední řadě také v oblasti molekulární biologie a genomiky (analýza nukleových kyselin a proteinů, microarray skenery a skla, PCR & RT-PCR, qPCR, řešení pro NGS, reagencie pro mutagenezi a klonování, sondy FISH a další).

Altium International

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Jsme jedním z lídrů na českém a slovenském trhu v oblasti laboratorních zařízení a svým zákazníkům přinášíme špičkové přístroje z oboru analytické chemie a fyzikálních měření více než 25 let.

Pragolab

Separation Science

Agilent Technologies

Thermo Fisher Scientific

SelectScience

SCIEX

Waters Corporation

<h4><strong>How about a 30 minute coffee break along with a taste of Empower?</strong></h4><h4><strong>Here you go!</strong></h4><p>Discover how to get more out of Empower by using Advanced Custom Fields.<br>In this short, example‑driven session, we will show how to build and apply Custom Fields that streamline calculations, improve reporting, and support data integrity.</p><h5><strong>You will learn:</strong></h5><ul><li>How to create and configure advanced Custom Fields</li><li>Practical examples (ratios, conditional logic, peak‑based calculations)</li><li>Best practices to avoid common errors and ensure validation‑ready results</li></ul><p><i>Join us to boost your Empower skills and make your data processing more efficient.</i></p><blockquote><p><strong>Presenter: </strong>Ralf Schröder (Informatics Market Development Manager)</p><p><strong>Presenter:&nbsp;</strong>Carsten Fischer (Informatics Product Marketing, EMEA)</p></blockquote>

Waters Corporation: Informatics series #31 Empower Advanced Custom Fields - Examples

Informatics series #31 Empower Advanced Custom Fields - Examples

Learn how to use Advanced Custom Fields in Empower to streamline calculations, improve reporting, and ensure validation-ready, compliant data processing.

Informatics series #31 Empower Examples

<h4><strong>Food Safety Webinar Series: When Food Meets the Lab</strong></h4><p><strong>Food testing in the region still revolves around “legacy” contaminants that decide release, rejection, and recall discussions - veterinary drug residues, pesticide residues and fumigants, and toxic elements including species-sensitive forms, in different food matrices. This 3-part series is built for audiences where regulatory expectations reference Codex-based frameworks, country-specific legislative guidelines, and import/export-market requirements that influence testing scope, reporting limits, and confirmation criteria. Each webinar focuses on the analytical factors that drive outcomes: matrix effects and co-extractives, trace-level quantitation, transition/ion-ratio checks, interference control, and reportable result logic.</strong></p><p>Keynote expert speakers will set the regulatory context, give a glimpse of the current trends, and future implications. Agilent workflow segments map these needs to sample preparation nuances, LC-MS/MS for multi-class residues, GC-MS/MS and headspace GC for residue/fumigant markers, and ICP-MS plus LC-ICP-MS for heavy metals and speciation, including QC structures and data review patterns used in high-throughput labs, to name a few.</p><h5><strong>Webinar Series Details</strong></h5><ul><li>Register once to attend the complete 3-part series</li><li>Submit your questions beforehand</li><li>Access recorded sessions anytime within one year</li><li>Certificate of attendance will be provided to all attendees</li></ul><h4><strong>Part 2: Pesticide Residues &amp; Fumigants Edition</strong></h4><p>Pesticide residue testing is shaped by Codex/EU-aligned expectations that frequently drive import decisions, while fumigant markers remain high-scrutiny topics for ingredients and finished foods, especially in spice, grain, seed, and produce supply chains. This webinar connects the regulatory context to method choices and result interpretation: broad-scope multi-residue coverage versus matrix complexity, QuEChERS-based extraction and cleanup, split-platform workflows (LC-MS/MS and GC-MS/MS). For fumigants, the focus includes sample handling for volatility, targeted measurement approaches for ethylene oxide/2-CE and phosphine-related testing where relevant, and reporting pitfalls that trigger disputes.&nbsp;</p><blockquote><p><strong>Presenter: </strong>Dr. Vandana Tripathy (Principal Scientist &amp; Network Coordinator, AINP on Pesticide Residues, ICAR-IARI; FSSAI Scientific Panel on Pesticide Residues, India)</p><p><strong>Presenter:&nbsp;</strong>Dr. Yu-Feng Zhang (Application Engineer, Agilent Technologies, Singapore)</p></blockquote>

Agilent Technologies: Food Safety Webinar Series: When Food Meets the Lab

Pesticide Residues & Fumigants Edition

Webinar on pesticide residues and fumigants: LC-MS/MS, GC-MS/MS, QuEChERS workflows and Codex/EU-aligned regulatory reporting.

<p><strong>Join us for this dual‑session SelectScience® webinar exploring hydrogen impurity testing and analytical strategies for achieving ISO 14687‑aligned fuel quality. To better suit your schedule and time zone, the webinar will be offered in two separate sessions:</strong></p><ul><li><strong>Session 1:</strong> 09:00 BST | 10:00 CEST</li><li><strong>Session 2: </strong>10:00 PDT | 13:00 EDT | 18:00 BST</li></ul><p>As hydrogen scales across energy, transport, and industrial applications, understanding fuel purity is fundamental for compliance, safety, and uptime. At present, only a limited number of instruments are capable of addressing the full hydrogen impurity matrix, meaning that most testing strategies continue to rely on a combination of analytical technologies aligned to the specific production pathway.</p><p>In this webinar, Tyler Roberts, Market Manager of Applied Markets at Shimadzu, presents a practical, process‑aware framework for hydrogen impurity testing. He will outline how different analytical technologies contribute to a complete workflow, including:</p><ul><li><strong>System GC</strong> for permanent gases, hydrocarbons, and sulfur compounds</li><li><strong>GC‑MS</strong> for organic impurity identification and speciation</li><li><strong>Ion chromatography</strong> for ammonia and halogenated acids at the lowest required detection limits</li><li><strong>FTIR</strong> for rapid multi‑component screening from a single sample</li></ul><p>Covering pathways from steam methane reforming (SMR) and electrolysis to ammonia cracking and liquid organic hydrogen carrier (LOHC) systems, this session will guide you in building the analytical foundation for confident hydrogen quality assurance benchmarked against ISO 14687.</p><h4><strong>Who should attend?</strong></h4><p>This event is perfect for:</p><ul><li>Analytical chemists</li><li>QC lab scientists</li><li>Hydrogen production process engineers</li><li>Fuel cell researchers</li><li>Fuel cell test engineers</li><li>Catalyst researchers</li><li>Lab managers</li><li>Lab procurement specialists</li></ul><h5><strong>Certificate of attendance</strong></h5><ul><li>If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</li><li><i>If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.</i></li></ul><h4><strong>Webinar details</strong></h4><ul><li><strong>Cost: </strong>Free to attend</li><li><strong>Location: </strong>Online</li><li><strong>Duration:</strong> 60 minutes</li></ul><p><i>Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available</i></p><blockquote><p><strong>Speaker: </strong>Tyler Roberts (Market Manager, Applied Markets, Shimadzu Scientific Instruments)</p><p>Tyler Roberts holds a Master’s in Analytical Chemistry from the University of Arizona and has built a reputation for bridging deep technical expertise with market-driven innovation. Tyler is now the Market Manager for Applied Markets at Shimadzu Scientific Instruments, where he leads technical marketing strategy across the energy, chemicals, and materials sectors and is a vocal advocate for advancing analytical science in high-impact industries.</p><p><strong>Moderator:</strong> Olivia Long (Editorial Team, SelectScience)</p></blockquote>

Shimadzu: What’s in your hydrogen? Analytical toolkits for fuel purity analysis

What’s in your hydrogen? Analytical toolkits for fuel purity analysis

Hydrogen impurity testing and ISO 14687 compliance. Discover GC, GC-MS, IC, and FTIR strategies for reliable fuel quality assurance.

Analytical toolkits for fuel purity analysis

<p><strong>In Part 2 of our three-part Chemoproteomics Master Class Series, we move beyond foundational strategies to explore real-world applications led by experts actively advancing the field. Dr. Mikhail Savitski will highlight the development and use of high-throughput peptide centric local stability assay (HT-PELSA) to map ligand binding sites and interactions. This screening strategy can provide insight to drug targets and specificity with peptide level resolution. Mikhail will give examples to show where this can be most effective in a drug discovery pipeline, and uncover new biology.</strong></p><h4><strong>Why Attend</strong></h4><ul><li>Gain insight into HT-PELSA, a cutting-edge assay paired with the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/836"><strong>Thermo Scientific™ Orbitrap™ Astral™ Mass Spectrometer</strong></a> to pinpoint ligand binding sites with peptide-level precision.</li><li>Learn how this method can enhance target discovery and specificity in your drug development pipelines.</li><li>See real-world examples of HT-PELSA uncovering new biology and accelerating chemoproteomic workflows.</li></ul><blockquote><p><span><strong>Presenter: </strong></span>Mikhail Savitski (Team Leader, Head of Proteomics Facility, and Senior Scientist, European Molecular Biology Laboratory (EMBL))</p><p>Mikhail Savitski, Ph.D., heads Functional Proteomics and the Proteomics Core Facility at EMBL Heidelberg. He is recognized for pioneering Thermal Proteome Profiling (TPP), enabling proteome-wide drug target discovery and protein stability studies. In 2024, he received the HUPO Discovery Award for his impact on proteomics research.</p><p><strong>Moderator: </strong>Brett Larsen (Sr. Marketing Specialist Proteomics, Thermo Fisher Scientific)</p><p>Brett Larsen is a leader in chemoproteomics and post-translational modification analysis at Thermo Fisher Scientific. With over 25 years of mass spectrometry experience, he has led proteomics at LTRI Toronto and advanced applications at MDS Proteomics. Brett brings practical expertise in translating complex workflows into real-world discovery.</p></blockquote>

Thermo Fisher Scientific: Powering drug discovery: virtual master class

From experts to applications: Screening protein-drug interactions with HT-PELSA

Part 2 of the Chemoproteomics Master Class shows how HT-PELSA with Orbitrap Astral MS reveals ligand binding sites for drug discovery.

Screening protein-drug interactions with HT-PELSA

<h4><strong>Demystifying PFAS, from fundamentals to first steps in the lab A webinar series for mass spec scientists and a practical training resource for lab managers building confident food testing teams</strong></h4><p>Per- and polyfluoroalkyl substances (PFAS) are everywhere, from drinking water to food packaging, and understanding how to detect and analyze them is more critical than ever. Whether you're just starting out or looking to sharpen your skills, our new four-part webinar series breaks down the essentials of PFAS analysis with expert-led sessions, practical tools, and real-world insights.</p><p>Designed with lab analysts and managers in mind, this series also serves as a structured training resource to onboard new analysts, deliver refresher learning, and build consistent competency across the team.</p><h4><strong>Webinar: PFAS first steps: A roundtable for new analysts</strong></h4><p><strong>Join our expert panel as they tackle common challenges faced by new analysts. From troubleshooting poor linearity to ensuring regulatory readiness, this roundtable is packed with practical advice and peer insights.</strong></p><h5><strong>What you’ll learn:</strong></h5><ul><li>Learn how experienced PFAS specialists troubleshoot the issues new analysts often face</li><li>Gain practical, real lab strategies to build confidence in your workflows</li><li>Understand what regulatory readiness looks like for early career analysts</li></ul><blockquote><p><strong>Host:</strong> Craig Butt, Senior Technical Manager, Food and Environmental</p><p>Craig M. Butt is Senior Manager of the Applied Markets group in the Global Technical Marketing division of SCIEX with over 20 years of mass spectrometry experience. Previously at SCIEX he was an applications scientist where he developed methods for targeted and non-targeted methods in the cannabis, environmental, food and beverage group. &nbsp;He obtained his Ph.D. in environmental chemistry at the University of Toronto, his thesis research investigated the fate of poly- and perfluorinated organic compounds (PFAS) in biological systems. &nbsp;He was a NSERC post-doctoral research fellow in the Nicholas School of the Environment at Duke University, later becoming a research scientist in the department.</p><p><strong>Panelist: </strong>Megumi Shimizu, LC-MS Application Scientist, SCIEX</p><p>Megumi Shimizu is a Senior Application Scientist on SCIEX’s Environmental Application Team, providing technical guidance, troubleshooting, and customized workflow development for LC‑MS. She holds a PhD in Marine Science from Duke University and has 8 years of experience focused on PFAS analysis and environmental contaminants. Megumi supports customers across nominal and high-resolution platforms, helping them develop sensitive, robust methods for PFAS detection, quantification, and characterization in complex environmental matrices.</p><p><strong>Panelist: </strong>Maryam Sayeed, Product Manager, Phenomenex</p><p>Maryam Sayeed is a Product Manager at Phenomenex and has been with the company for three years. She focuses on expanding solutions for chromatography products and consumables designed for the food and environmental markets. Her work also includes supporting products that address challenges associated with PFAS analysis and background contamination at low detection limits. Prior to joining Phenomenex, Maryam spent nine years in the food and beverage industry, gaining extensive hands‑on experience with HPLC analysis. She holds an MS in Biology with a specialization in Biochemistry.</p><p><strong>Panelist: </strong>Simon Roberts, Staff Applications Scientist, SCIEX</p><p>As a postdoctoral fellow at the Colorado School of Mines, Simon worked with Dr. Christopher Higgins and Dr. Jennifer Field to identify novel classes of PFAS chemicals in aqueous film-forming foams (AFFFs). At SCIEX, Simon specializes in method optimization and data reporting as he helps labs around the country set up PFAS and other environmental analytical methods.</p><p>Simon has spent nearly 15 years researching the distribution of PFAS in the environment. He has used his expertise to set up, optimize, and troubleshoot PFAS analysis at many major commercial, government, and academic environmental labs across North America. Simon lives in Denver and enjoys getting lost in the mountains and playing music</p></blockquote>

SCIEX: Back to PFAS basics: LC-MS/MS essentials

PFAS first steps: A roundtable for new analysts

PFAS webinar for new analysts: troubleshooting, workflows and regulatory readiness with expert panel insights.

<p><strong>This webinar highlights a fully automated 2D LC-MS workflow for rapid, high-coverage mRNA characterization. By integrating online partial RNase T1 digestion directly into a 2D LC setup, the method enables controlled, reproducible digestion with seamless transfer into ion-pair reversed-phase separation.</strong></p><h4><strong>The result:</strong></h4><ul><li>93–99% sequence coverage in &lt;60 minutes</li><li>No post-digest sample handling</li><li>Automated multi-attribute monitoring (sequence identity, 5’ cap, poly(A) tail)</li></ul><p>The 2D LC configuration is central to achieving speed, resolution, and robustness in mRNA analysis.</p><h4><strong>Key Learnings:</strong></h4><ul><li>How 2D LC integrates online RNase digestion</li><li>Why 2D LC improves separation and sequence coverage</li><li>Benefits of automation for reproducibility and throughput</li><li>Enabling multi-attribute mRNA analysis in a single workflow</li></ul><h5><strong>Who should attend?</strong></h5><ul><li>mRNA analytics &amp; LC–MS scientists (analytical development, characterization): especially anyone doing mRNA sequence mapping, oligoribonucleotide ID, or improving coverage/speed with LC–MS workflows.</li><li>Biopharma QC / QC method owners working on mRNA identity testing and routine attribute measurements where automation, robustness, and reproducibility matter.</li><li>Process development / CMC / MSAT teams supporting mRNA modalities who need faster turnaround data on critical quality attributes (CQAs) like 5’ cap, poly(A) tail length/heterogeneity, and sequence identity.</li><li>Platform/automation &amp; high-throughput labs looking to reduce hands-on time and eliminate post-digest sample handling while increasing throughput.</li><li>Chromatography / LC system specialists interested in 2D LC configuration, online enzymatic digestion integration, and ion-pair reversed-phase separations for challenging RNA samples.</li><li>R&amp;D leaders / assay strategists evaluating next-gen, multi-attribute monitoring approaches that consolidate multiple measurements into one automated 2D LC–MS method.</li></ul><blockquote><p><strong>Speaker:</strong> Jessica Dale (PhD Student in Oligonucleotide Analysis, University of Sheffield)</p></blockquote>

Thermo Fisher Scientific: Automated direct mRNA sequence mapping using online partial RNase T1 digestion and 2D LC-MS

Automated direct mRNA sequence mapping using online partial RNase T1 digestion and 2D LC-MS

How 2D LC integrates online RNase digestion

Automated direct mRNA sequence mapping

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Per- and polyfluoroalkyl substances (PFAS) are a large class of synthetic compounds valued for their chemical stability and resistance to water and oil. Widely used in industrial processes, firefighting foams, and consumer products, PFAS have become persistent contaminants in both industrial and municipal waste streams. As a result, wastewater utilities and industrial dischargers face increasing pressure to detect and manage PFAS in wastewater and biosolids.</strong></p><p>These matrices are highly variable, ranging from liquid influent and effluent with suspended solids to partially or fully dried biosolids. This variability can significantly impact extraction efficiency, reproducibility, and overall method robustness.</p><p>In this webinar, we will explore key strategies laboratories use to overcome matrix challenges, including advanced sample preparation, isotope dilution with extracted internal standards, and improved chromatographic separation.</p><p>Understanding matrix complexity is essential for producing reliable data. Depending on the sample, additional steps may be required to meet state reporting limits or project-specific data quality objectives. Join us to learn how robust analytical approaches can deliver confident results, even in the most challenging wastewater and biosolid samples.</p><h5><strong>By attending this webinar, you will:</strong></h5><ul><li>Gain a clear understanding of how sample matrix variability impacts PFAS analysis in wastewater and biosolids, and the key factors that influence data quality.&nbsp;</li><li>Learn practical strategies, including sample preparation and analytical techniques, to improve robustness and reproducibility.&nbsp;</li><li>Receive guidance on producing defensible results that meet regulatory and project-specific requirements.</li></ul><h5><strong>Who should attend?</strong></h5><ul><li>Environmental and commercial testing laboratories performing PFAS analysis</li><li>Wastewater utilities and municipal laboratories managing PFAS compliance</li><li>Industrial facilities and consultants responsible for PFAS monitoring and treatment&nbsp;</li><li>Regulatory agencies and researchers focused on environmental contaminants and water quality</li></ul><blockquote><p><strong>Presenter: </strong>Sarah Choyke, PhD (Technical Director, Eurofins Environment Testing, Denver, Colorado, USA)</p><p>Sarah Choyke brings over a decade of experience in environmental analysis with expertise in LC-HRMS, LC-MS/MS, and method development for contaminants of emerging concern, including PFAS, flame retardants, and personal care products. She has extensive experience analysing PFAS at industrial and AFFF impacted sites using targeted and nontargeted analysis. Dr Choyke has nine peer-reviewed research articles and over a dozen conference presentations. In her role as Technical Director, she is expanding the PFAS capabilities in Denver and upgrading analytical techniques to help improve efficiency.</p></blockquote></div></div></div>

SeparationScience: Testing PFAS in Biosolids: From Sample Preparation to Interference Separation

Testing PFAS in Biosolids: From Sample Preparation to Interference Separation

Learn how robust analytical approaches can deliver confident results, even in the most challenging wastewater and biosolid samples.

Testing PFAS in Biosolids

<p><strong>Modern laboratories require faster workflows, reliable data processing, and strict regulatory compliance. In this webinar, we will explore how SCIEX OS software simplifies and automates key mass spectrometry workflows, from data acquisition to analysis and compliant reporting.</strong></p><p>Join us to discover how the powerful capabilities of SCIEX OS Software help laboratories improve efficiency, reduce manual steps, and maintain data integrity. With its integrated environment for instrument control, data processing, and reporting, SCIEX OS software helps laboratories move seamlessly from sample to result—faster, smarter, and with confidence.</p><h4><strong>Key highlights:</strong></h4><ul><li>Overview of acquisition, processing, and reporting in SCIEX OS software with automation</li><li>Compliance, validation, and audit trails</li><li>Custom calculations for confident data analysis</li></ul><blockquote><p><strong>Speaker:</strong> Raviraj Landage, Application Support Specialist</p></blockquote>

SCIEX: Unlocking SCIEX OS software: automating acquisition, analysis, and 21 CFR part 11 compliance reporting

Unlocking SCIEX OS software: automating acquisition, analysis, and 21 CFR part 11 compliance reporting

Join us to discover how powerful capabilities of SCIEX OS Software help laboratories improve efficiency, reduce manual steps, and maintain data integrity.

SCIEX OS software automating acquisition

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Looking to further elevate your Thermo Scientific Chromeleon CDS skills? Want to learn more about the administration tools for your Chromeleon software setup? Already attended Administration Console I or II webinar (tips, shortcuts, and user/access management)? Part III is the next step – focused on licensing and data management across your Chromeleon software environment.</strong></p><p>In this practical session, we’ll dive into more Administration Console capabilities that help you manage and standardize your setup at scale – from Query creation and Scheduler usage for data transfer to Network Failure Protection and data management. You’ll leave with proven approaches for keeping multi-station environments consistent, controlled, and easier to support.</p><h4><strong>What’s different in Part 3 (vs. Part 1 &amp; 2):</strong></h4><blockquote><ul><li><strong>Part 1: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/5981"><strong>Expert Tips and Tricks</strong></a></li><li><strong>Part 2: </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6126"><strong>System management and automation</strong></a></li></ul></blockquote><ul><li>Beyond users &amp; permissions: Shift from access management to managing your data within and between Data Vaults.</li><li>Network Failure Protection: Learn how to set the system up for maximum resilience to network outages using the latest tools to ensure data security and lab uptime.</li><li>Data management: Explore methods for transferring data automatically to backup and archive, reducing manual touchpoints and managing data effectively.</li><li>Real-world best practices: Practical guidance to help maintain stability, security, and compliance as environments grow.</li></ul><h4><strong>Who should attend:</strong></h4><ul><li>This session is designed for anyone wanting to learn more about administration of Chromeleon CDS, administrators, lab IT/support teams, and power users who want to take on administration responsibilities – especially those supporting multiple stations or managing standardized deployments.</li></ul><p>Get practical, immediately usable techniques to keep the Chromeleon software setup scalable, consistent, and supportable – with less manual effort and fewer surprises.</p><p><i>Cannot attend the live broadcast on April 23? Please register anyway to automatically receive access to the recording.</i></p><blockquote><p><strong>Presenter: </strong>Shakeil Akhtar (Sr. Engineer, Field Applications, Thermo Fisher Scientific)</p></blockquote></div></div></div>

Thermo Fisher Scientific: Chromeleon Admin Console III: Data management and automation

Chromeleon Admin Console III: Data management and automation

In this practical session, we’ll dive into more Administration Console capabilities that help you manage and standardize your setup at scale.

<p><strong>LC analysis of many biomolecules remains challenging due to their sensitivity to metal interactions within chromatographic systems, often leading to poor peak shape and reduced sensitivity in chromatographic separations.</strong></p><p>This webinar explores how <strong>Agilent Altura HPLC columns with Ultra Inert technology</strong> overcome these challenges by minimizing metal-analyte interactions. We will illustrate how this innovation enhances resolution, reproducibility, and quantification in reversed phase and HILIC separations of oligonucleotides and synthetic peptides.</p><h4><strong>Who should attend?</strong></h4><ul><li>Laboratory technicians, researchers, and scientists wishing to learn more about peptide and oligonucleotide &nbsp;behavior in columns with inert technology, enhancing method development, and streamlining analytical processes.</li><li>Anyone looking to gain a better understanding of strategies that can be implemented to improve your peptide and oligonucleotide analysis workflows and data quality.</li></ul><blockquote><p><strong>Presenter:</strong> Katrien Sabbe, PhD (Biopharma Business Development Manager Chemistries and Supplies, Agilent Technologies)</p><p>Katrien Sabbe holds a PhD in organic synthesis (Ghent University, Belgium). For more than 25 years she is working in the field of Chromatography Columns and Supplies, first at VWR International and later at Varian Analytical Instruments, which was acquired in 2011 by Agilent Technologies. Currently she is the EMEA Biopharma Business Development Manager at Agilent Technologies Chemistries and Supplies Division.</p></blockquote>

Agilent Technologies: Enhancing Peptide and Oligonucleotide Analysis with Ultra Inert HPLC Technology

Enhancing Peptide and Oligonucleotide Analysis with Ultra Inert HPLC Technology

Webinar on ultra-inert HPLC columns for peptide and oligonucleotide LC analysis. Improve peak shape, sensitivity, and reproducibility with Agilent Altura.

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HALO® BIOCLASS Oligonucleotide kolony

Jak vybrat správnou SEC kolonu pro analýzu biologických molekul

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