Tento technický přehled popisuje systematický přístup k řešení jednoho z nejčastějších problémů v kapalinové chromatografii: posuny retenčních časů. Metodika je založena na rozlišení mezi problémy systému (fyzikálními) a chromatografickými (chemickými) příčinami.Klíčovým diagnostickým parametrem je T₀ (mrtvý čas), který představuje nezadržený pík.<h3>Klíčový koncept: T₀ (mrtvý čas / nezadržený pík)</h3>T₀ je retenční čas látky, která neinteraguje se stacionární fází a prochází kolonou bez zdržení.<h4>Stanovení T₀</h4>Experimentálně lze T₀ určit přídavkem markeru:<ul><li>Uracil</li><li>Dusičnan draselný</li></ul>Tyto látky se přidávají do blanku nebo metody a vytvářejí měřitelný nezadržený pík využitelný pro účely troubleshootingu.<h3>Krok 1: Vyhodnocení chování T₀</h3><ul><li>Varianta A: T₀ se mění (není konstantní) → Problém systému (fyzikální) &nbsp;</li><li>Varianta B: T₀ je konstantní → Chromatografický / chemický problém</li></ul><h3>Problémy systému (T₀ se mění)</h3>Pokud se T₀ posouvá, je problém pravděpodobně spojen s hardwarem LC systému nebo fyzikálními parametry. To platí zejména v případě, kdy se všechny píky v chromatogramu posouvají stejným směrem.<h4>Možné příčiny</h4><ul><li>Nesprávný průtok</li><li>Porucha pumpy<ul><li>nestabilní nebo kolísavý průtok a může vést k variabilitě retenčních časů</li></ul></li><li>Nesprávná délka kolony<ul><li>použití kolony s odlišnými rozměry, než je očekáváno</li></ul></li><li>Problémy se solventem (např. prázdná láhev)</li><li>Ucpání (frit, kapiláry)</li><li>Změny v objemu systému (kapiláry, konfigurace)<ul><li>změna konfigurace kapilár</li><li>změna systémového objemu (dwell/swell volume)</li></ul></li></ul><h3>Chromatografické / chemické problémy (T₀ je konstantní)</h3>Pokud je T₀ stabilní, ale dochází k posunu pouze u vybraných píků, jedná se pravděpodobně o problém související s chemií systému nebo vlastnostmi kolony.<h4>Možné příčiny</h4><ul><li>Nesprávná stacionární fáze<ul><li>kolona má stejné rozměry, ale odlišnou chemii (např. jiný typ siliky nebo navázané fáze)</li></ul></li><li>Ionizovatelné analyty (nejčastější případ) Ionizovatelné látky existují ve dvou formách:<ul><li>Neutrální (více hydrofobní → vyšší retence)</li><li>Ionizovaná</li></ul>Vliv pH Malé změny pH → velké změny retenčního času</li><li>Kvalita mobilní fáze (stárnutí, odpařování)</li><li>Teplota<ul><li>teplota ovlivňuje interakce mezi analytem a stacionární fází</li><li>i malé výkyvy mohou způsobit změny retenčních časů</li></ul></li></ul><h3>Postup řešení</h3><ol><li>Přidat marker T₀<ol><li>např. uracil nebo dusičnan draselný</li></ol></li><li>Vyhodnotit stabilitu T₀<ol><li>zjistit, zda je konstantní nebo variabilní</li></ol></li><li>Při variabilním T₀ → kontrola systému<ol><li>nastavení průtoku</li><li>funkce pumpy</li><li>hladina a přívod mobilní fáze</li><li>rozměry kolony</li><li>kapiláry a objem systému</li></ol></li><li>Při konstantním T₀ → kontrola chemie<ol><li>typ stacionární fáze</li><li>složení a pH mobilní fáze</li><li>kvalita solventů</li><li>stabilita teploty</li><li>vlastnosti analytů (ionizovatelnost)</li></ol></li></ol><h3>Diskuze</h3>Tento přístup zjednodušuje řešení problémů tím, že rozděluje jejich příčiny do dvou hlavních kategorií:<ul><li>fyzikální / systémové</li><li>chemické / chromatografické</li></ul>Využití T₀ jako diagnostického nástroje umožňuje rychle identifikovat původ problému a minimalizovat zbytečné pokusy metodou pokus–omyl.Zvláštní pozornost je nutné věnovat ionizovatelným sloučeninám, které jsou velmi citlivé na změny pH, což může vést k výrazným a selektivním posunům retenčních časů.<h3>Závěr</h3>Posuny retenčních časů v LC lze efektivně diagnostikovat sledováním chování T₀:<ul><li>Variabilní T₀ → problém systému</li><li>Konstantní T₀ → chromatografický / chemický problém</li></ul>Díky tomuto strukturovanému přístupu lze rychle identifikovat příčinu problému a provést cílená nápravná opatření.

Řešení problémů se změnami retenčního času v kapalinové chromatografii

Vědci z CEITEC Masarykovy univerzity spolu s&nbsp;kolegy z&nbsp;USA zjistili, že bakterie mají zásadní slabinu: bez proteinu RimM se při nedostatku živin nebo po stresu obnovují jen velmi pomalu. Klíčovou roli v&nbsp;jejich opětovném množení hraje správné sestavení ribozomů – buněčných továren na bílkoviny. Zjištění otevírá novou možnost, jak bakteriím narušit schopnost znovu se začít dělit, což může medicíně v budoucnu pomoci lépe bojovat s infekcemi.Každá bakterie má jednu zásadní schopnost – když se dostane do nepříznivých podmínek, dokáže se utlumit a nepříznivé období přečkat. Jakmile se podmínky zlepší, rychle znovu nastartuje svou aktivitu. A právě v tom vědci z CEITEC Masarykovy univerzity objevili Achillovu patu bakterií.Vědecký tým se zaměřil na bakterii Escherichia coli a zjistil, že bez proteinu RimM se její buňky po stresu zotavují výrazně hůř. Obnova jejich buněčného dělení je v&nbsp;takovém případě pomalá, protože protein RimM se podílí na sestavování ribozomů – struktur nezbytných pro tvorbu bílkovin klíčových pro život buňky. Bez něj se bakterie po stresové zátěži množí mnohem pomaleji.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_199d25ed9b.jpg" alt="CEITEC/Demo Lab: Achillova pata bakterií – bez klíčového proteinu se množí pomalu: Kryo-EM mikrograf ribozomálních částic rozptýlených v tenké vrstvě amorfního ledu." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_199d25ed9b.jpg 207w," sizes="100vw" width="1600" height="1205">Vědci pomocí kryo-elektronové mikroskopie sledovali, jak se ribozomy bez proteinu RimM chovají. Ukázalo se, že se skládají pomalu a chaoticky – především v&nbsp;místě, které je klíčové pro přesné čtení genetické informace – a buňka je proto držena v jakémsi „čekacím módu“. „Zjistili jsme, že protein RimM je pro bakterie mnohem důležitější, než se dosud myslelo,“ říká první autor <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.cz/ahmed-adel-ibrahim-hassona-hassan-dr-rer-nat/u94046?page=publication">Ahmed H. Hassan</a>. „Pokud chybí, bakteriální buňka se velmi obtížně vrací k normální činnosti, protože ribozomy dozrávají jen pomalu.“<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_3_1e27130702.jpeg" alt="CEITEC: Achillova pata bakterií – bez klíčového proteinu se množí pomalu: Ahmed H. Hassan." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_3_1e27130702.jpeg 235w," sizes="100vw" width="1600" height="1067">Výzkum také odhalil, že bakterie v této situaci aktivují ochranný mechanismus, který brání předčasnému spojení nedokončených částí ribozomu – aktivují se další proteiny, které celý proces raději zpomalí, aby zabránily vzniku vadných ribozomů. Bakterie se tak sice nakonec vzpamatuje, ale mnohem později, než je běžné. Právě tuto slabinu, kdy bakterie bez RimM fungují ve zpomaleném režimu, lze do budoucna využít pro nové léčebné postupy v&nbsp;boji proti bakteriálním infekcím.Protein RimM je běžný u bakterií, ale nevyskytuje se u člověka, což je pro vývoj léčiv, jako jsou například antibiotika, velká výhoda. Pokud vědci vyvinou lék, který by tento protein zablokoval, zasáhne pouze bakterie, ne lidské buňky. „Takové léčivo by mohlo bakteriím výrazně ztížit zotavení po stresu způsobeném právě antibiotiky, a tím omezit jejich další šíření, aniž by mělo vedlejší účinky pro pacienta,“ vysvětluje vedoucí výzkumu <a target="_blank" rel="noopener noreferrer" href="https://www.ceitec.cz/mgr-gabriel-demo-ph-d/u92825">Gabriel Demo</a> širší význam výsledků.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_4_474572c812.jpeg" alt="CEITEC/Martin Indruch: Achillova pata bakterií – bez klíčového proteinu se množí pomalu: Gabriel Demo." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_4_474572c812.jpeg 235w," sizes="100vw" width="1600" height="1067">Studie, na které spolupracovali také vědci z Thomas Jefferson University, Columbia University a University of California Riverside, byla publikována v časopise <a target="_blank" rel="noopener noreferrer" href="https://www.nature.com/articles/s41467-026-71781-6">Nature Communications</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_2_d3266ee9ec.jpg" alt="CEITEC/Ahmed H. Hassan: Achillova pata bakterií – bez klíčového proteinu se množí pomalu: Model sestavování ribozomu s objevenými faktory (červeně a modře), které podporují jeho vznik v nepřítomnosti RimM." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CEITEC_Achillova_pata_bakterii_bez_klicoveho_proteinu_se_mnozi_pomalu_2_d3266ee9ec.jpg 103w," sizes="100vw" width="1600" height="2423">

 Achillova pata bakterií – bez klíčového proteinu se množí pomalu 

V týdnu od 4. května na Vás čekají v oblasti kapalné fáze a především HPLC a LC/MS následující webináře.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://www.lcms.cz/webinars">👉 DETAILY A REGISTRACE V SEKCI WEBINÁŘE</a></h4></blockquote><blockquote>👉 Vyhledávejte a filtrujte v největší globální databázi s více než 5 500 GC, HPLC, MS a spektroskopickými webináři.</blockquote><h5>1. Merck: Recent Advances in Chromatographic Approaches in Characterizing Antibody-Drug Conjugates</h5><ul><li>Út, 5.5.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6146">Registrace</a></li></ul>Pokročilé strategie LC-MS a HIC pro komplexní charakterizaci ADC, včetně profilování nečistot, analýzy DAR a MS-kompatibilních workflow.<h5>2. Waters Corporation: Empower Tips: Ask Me Anything About Updates in Empower Software to Advance Your Compliance</h5><ul><li>Út, 5.5.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5975">Registrace</a></li></ul>Nové funkce Empower CDS pro zlepšení compliance, bezpečnosti, výpočtů USP a řízení rizik v chromatografických laboratořích.<h5>3. Metrohm: Electrochemiluminescence: The technique that should be on your radar for sensor development</h5><ul><li>St, 6.5.2026 09:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6158">Registrace</a></li></ul>Úvod do ECL jako výkonné techniky pro vývoj senzorů s praktickými tipy a aplikacemi.<h5>4. Phenomenex: How To Start Up and Shut Down LC and LC-MS Instruments</h5><ul><li>St, 6.5.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6180">Registrace</a></li></ul>Praktické postupy pro spouštění a vypínání LC/LC-MS systémů, údržbu kolon a kontrolu výkonu systému.<h5>5. Thermo Fisher Scientific: Integrated LC-MS CDS workflow for automated ADC and DAR analysis</h5><ul><li>St, 6.5.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6135">Registrace</a></li></ul>Automatizovaný LC-MS workflow pro analýzu intaktních ADC a DAR pomocí Orbitrap Exploris 240 v nativních podmínkách.<h5>6. Waters Corporation: Improving Sensitivity and Throughput in Early Drug Development with Inert LC Technologies</h5><ul><li>St, 6.5.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6152">Registrace</a></li></ul>Jak kolony MaxPeak Premier zvyšují citlivost, robustnost a kvalitu LC analýz v rané fázi vývoje léčiv.<h5>7. Thermo Fisher Scientific: Keeping Up with Evolving Regulatory Requirements: Addressing Analytical Challenges for Known and Unknown Nitrosamines</h5><ul><li>Čt, 7.5.2026 05:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6166">Registrace</a></li></ul>Strategie LC-MS pro kvantifikaci nitrosaminů a detekci neznámých nečistot v souladu s regulatorními požadavky.<h5>8. Waters Corporation: Transform Your Daily Lab Operations with Automation - Proteomics Workflows</h5><ul><li>Čt, 7.5.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6165">Registrace</a></li></ul>Automatizace manipulace s kapalinami pro vyšší reprodukovatelnost a efektivitu proteomických workflow.<h5>9. SCIEX: Assessing biotherapeutic stability through forced degradation using intact and subunit icIEF-UV/MS workflows</h5><ul><li>Čt, 7.5.2026 11:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6181">Registrace</a></li></ul>icIEF-UV/MS přístupy pro studium stability bioterapeutik, variant náboje a charakterizaci mAb.<h5>10. Metrohm: Environmental waters: A new generation of high-capacity IC columns makes analysis more efficient</h5><ul><li>Čt, 7.5.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6155">Registrace</a></li></ul>Kolony IC nové generace pro robustní a efektivní analýzu environmentálních vod.<h5>11. Waters Corporation: Add a New Dimension - MS Imaging for Environmental Analysis</h5><ul><li>Čt, 7.5.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5927">Registrace</a></li></ul>MS imaging a DESI pro pokročilou environmentální analýzu a prostorové informace o vzorcích.<h5>12. US HUPO: Raising the Bar: Quality Assurance and Data Integrity in MALDI MSI</h5><ul><li>Čt, 7.5.2026 19:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/6133">Registrace</a></li></ul>Osvědčené postupy pro QA a integritu dat v MALDI MSI pro reprodukovatelné prostorové omické analýzy.<h5>13. Agilent Technologies: Impurity Characterization of GLP-1 Receptor Agonists via RPLC and HILIC</h5><ul><li>Čt, 7.5.2026 19:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5998">Registrace</a></li></ul>Strategie RPLC a HILIC pro profilování nečistot GLP-1 a optimalizaci separace v souladu s regulací.<h5>14. Agilent Technologies: Accelerating LC/MS Impurity Screening Followed by ECD-Based Sequence Analysis for GLP-1 Analogs</h5><ul><li>Pá, 8.5.2026 11:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5945">Registrace</a></li></ul>LC/MS workflow pro rychlý screening nečistot GLP-1 a následnou sekvenční analýzu pomocí ECD.

Webináře LabRulezLCMS týden 19/2026

Webináře

Akademie

Věda a výzkum

Video

Článek

Je výzkumné centrum zaměřené na oblast živých věd, pokročilých materiálů a technologií.  Vznikl v roce 2011 na základech šesti významných brněnských univerzit a výzkumných institucí. Od doby jeho nedávného vzniku se daří budovat významné vědecké centrum, a již nyní se řadí mezi špičkové evropské instituce.

CEITEC

Vše ze světa analytické chemie na jednom místě. Propojujeme lidi při řešení jejich problémů. U nás naleznete všechny potřebné informace snadno, rychle a přehledně. Přestaňte hledat a začněte nacházet.

LabRulez

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Metrohm

Merck

Thermo Fisher Scientific

SCIEX

Waters Corporation

<div><h4>Unlock the Power of the Latest Empower CDS Releases</h4>Since the base release of Empower Chromatography Data System (CDS) in 2010, we have gone through 36 version updates. These updates have included new features, enhancements to existing functionality, and numerous updates across multiple areas - from instrument support to infrastructure technology.In this webinar we will focus on new features and updates in recent releases impacting compliance, an extremely important topic for many of you.<h5>What you’ll understand by the end</h5><ul><li>Risks of using outdated software</li><li>Support status of various Empower Software versions</li><li>Security enhancements to Empower CDS</li><li>Recent updates in USP calculations</li><li>Risk reduction of potential bias in the laboratory</li><li>Streamline the lab with Multi Angle Light Scattering and monitoring application</li></ul><h5>Who should attend</h5><ul><li>This webinar is designed for Lab Managers, Empower System Administrators, Quality, Quality IT, and Lab Personnel.</li></ul>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.<blockquote>Presenter: Neil Lander (Senior Principal Product Manager, Waters Corporation)Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Senior Principal Product Manager focusing on Informatics solutions.He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives.</blockquote></div>

Waters Corporation: Empower Tips: Ask Me Anything About Updates in Empower Software to Advance Your Compliance

Empower Tips: Ask Me Anything About Updates in Empower Software to Advance Your Compliance

Webinar on recent Empower CDS releases, covering compliance updates, security enhancements, and laboratory risk reduction strategies.

Updates in Empower Software to Advance Compliance

Antibody-drug conjugates (ADCs) require robust characterization of key quality attributes—especially the drug-to-antibody ratio (DAR)—to support confident decisions in discovery, development, and manufacturing.In this webinar, we’ll present an automated, high-throughput intact ADC workflow using LC-MS under native conditions on the Thermo Scientific Orbitrap Exploris 240 mass spectrometer, fully integrated within Thermo Scientific Chromeleon CDS.You’ll see how a single, unified platform can bring together data acquisition, automated intact deconvolution, DAR calculation, and reporting—reducing manual handoffs and analyst intervention while improving consistency and throughput. Whether you are scaling ADC characterization, standardizing DAR reporting, or looking to streamline intact analysis, this session will highlight a practical, end-to-end approach designed for efficient routine use.<h4>Key learning objectives:</h4><ul><li>Why intact ADC analysis (native LC–MS) is valuable for monitoring ADC heterogeneity and DAR-related attributes</li><li>How high-resolution mass spectrometry (HRMS) supports confident intact mass measurements for ADC characterization</li><li>What an end-to-end Chromeleon CDS workflow looks like—from instrument control to automated processing and reporting</li><li>How automated intact deconvolution enables rapid, consistent interpretation of intact ADC datasets</li><li>Approaches to automated DAR calculation and standardized reporting to support high-throughput workflows and data integrity</li></ul><h4>Who should attend?</h4><ul><li>ADC analytical characterization scientists in biopharma/biotech (discovery through late-stage)</li><li>LC-MS / HRMS users developing or running intact protein and ADC methods</li><li>CMC, process development, and QC teams interested in scalable DAR characterization workflows</li><li>Lab automation and informatics professionals focused on integrating acquisition, processing, and reporting</li><li>Chromeleon CDS users looking to expand into integrated intact deconvolution and reporting</li></ul>Cannot attend the broadcast on May 6? Please register to automatically receive the link to the recording.<blockquote>Speaker: Dr. Ulrik H. Mistarz (Application Scientist, Thermo Fisher Scientific)Dr. Ulrik Mistarz is a Senior Application Scientist at Thermo Fisher Scientific in Copenhagen, Denmark. He supports a broad range of LC-MS applications with a focus on developing novel workflows for characterization of oligonucleotides, therapeutic proteins, as well as different antibody formats.He has a strong interest in improving the throughput and automation of analytical workflows through developing new LC-MS as well as software workflows.Ulrik Mistarz has a PhD from The Department of Pharmacy at the University of Copenhagen and has more than 10 years of experience with pharma/biopharma characterization and analytics. This includes positions in both pharmaceutical and biotech companies working on LC-MS and HPLC application development.</blockquote>

Thermo Fisher Scientific: Integrated LC-MS CDS workflow for automated ADC and DAR analysis

Integrated LC-MS CDS workflow for automated ADC and DAR analysis

Webinar, will present automated intact ADC workflow using LC-MS under native conditions on the Thermo Scientific Orbitrap Exploris 240

LC-MS CDS workflow for automated ADC and DAR analysis

Antibody-drug conjugates (ADCs) represent a rapidly growing class of biotherapeutics that combine the specificity of monoclonal antibodies with the cytotoxic potency of small molecule drugs. Comprehensive characterization of ADCs is critical for ensuring product quality, efficacy, and safety, requiring advanced analytical techniques capable of resolving their inherent structural complexity and heterogeneity.This webinar presents state-of-the-art liquid chromatography-mass spectrometry (LC-MS) approaches for ADC characterization. The first half focuses on recent advances in reversed-phase LC-MS analysis, highlighting improvements in separation efficiency and detection sensitivity. Application examples will demonstrate the determination of low concentration impurities and variants, the effects of mobile phase and mobile phase modifiers on chromatographic performance, and the performance gains to the method by utilizing unique column geometries (1.5 mm I.D.).The second half explores native ADC analysis by hydrophobic interaction chromatography (HIC). Modern HIC stationary phases, combined with targeted mobile‑phase engineering, demonstrate high‑resolution, reproducible DAR separations across multiple ADC modalities, including improved resolution of high‑DAR and low‑abundance species. This state-of-the-art stationary phase was used to develop an HIC workflow using ammonium tartrate to achieve ammonium‑sulfate‑like performance and enabling rapid, MS‑compatible, and PAT‑ready ADC characterization.Attendees will gain practical insights into selecting appropriate LC-MS methodologies for their ADC characterization needs and understanding the complementary nature of reversed-phase and hydrophobic interaction chromatography.<h4>During this webinar, you will:</h4><ul><li>Discover the latest advancements in reversed-phase liquid chromatography-mass spectrometry (LC-MS) for the effective characterization of antibody-drug conjugates (ADCs), focusing on improved separation efficiency and detection sensitivity.</li><li>Learn about practical applications of LC-MS in identifying low concentration impurities and variants, including the impact of mobile phase components on chromatographic performance.</li><li>Explore innovative techniques in hydrophobic interaction chromatography (HIC) for native ADC analysis, emphasizing high-resolution and reproducible drug-to-antibody ratio (DAR) separations.</li><li>Gain an understanding about the development of a streamlined HIC workflow utilizing ammonium tartrate, which enhances ADC characterization efficiency while maintaining compatibility with mass spectrometry and process analytical technology (PAT).</li></ul><blockquote>Presenter: Andrea Krumm (Product Manager, Analytical Columns, Tosoh Bioscience GmbH)Andrea Krumm studied biotechnology before gaining a PhD in Cell and Cancer Biology. After four years as an applications specialist for optical analytical devices used in academic and pharmaceutical research, she joined Tosoh Bioscience GmbH in 2020 as a product manager for analytical columns. Andrea is responsible for gathering customer requirements, application areas and addressing these with new and existing products of the analytical columns line.Presenter: Cory E. Muraco (Senior Global Product Manager, Large Molecule HPLC, MilliporeSigma, the life science business of Merck KGaA, Darmstadt, Germany)Cory E. Muraco is the Senior Global Product Manager for Large Molecule HPLC Columns at MilliporeSigma, the life science business of Merck KGaA, Darmstadt, Germany. After finishing his graduate studies at Youngstown State University (Youngstown, Ohio, USA), Cory started his career at MilliporeSigma in 2013. After holding several roles in both R&amp;D and Marketing functions across the past decade, in 2026, Cory assumed his current role as the Large Molecule HPLC Columns Product Manager where he is tasked with designing, developing, and executing the marketing and R&amp;D strategies around MilliporeSigma’s biomolecule HPLC columns. Cory is the author of several manuscripts appearing in trade magazines and has delivered over 100 presentations at international conferences, round table symposia, and at various pharmaceutical and biopharmaceutical companies.</blockquote>

Tosoh Bioscience: Recent Advances in Chromatographic Approaches in Characterizing Antibody-Drug Conjugates

Recent Advances in Chromatographic Approaches in Characterizing Antibody-Drug Conjugates

Discover LC-MS and HIC methods for ADC characterization, including DAR analysis, impurity detection, and high-resolution separations for biopharma workflows.

Chromatographic Characterizing Antibody-Drug Conjugates

In the second webinar of the Small Molecules, Big Impact series, Ken Berthelette will demonstrate how Waters™ solutions and MaxPeak Premier Columns can be applied to improve LC discovery and early development workflows.This webinar will focus on applied case studies in early drug research development and demonstrate how enhanced surface technology can deliver improved sensitivity and peak shape for acidic analytes, helping teams generate higher-quality data earlier in development process. By focusing on specific applications of method development, this session will highlight how through intelligent column selection and process introduction, development workflows can be streamlined to generate the highest quality data possible.<h4>Who should attend</h4><ul><li>Analytical scientists who support early development workflows.</li></ul><h4>Why you should attend</h4><ul><li>Gain a clearer understanding of how MaxPeak Premier Columns can enhance LC workflows through improved sensitivity, peak shape, and robustness for acidic analytes.</li><li>Access resources and tools that enable independent exploration and evaluation of MaxPeak Premier technology within your own laboratory workflows.</li></ul><h4>What you will walk away with</h4><ul><li>A better understanding of MaxPeak Premier technology to gain the advantage in your research</li><li>Insights into how MaxPeak Premier Columns can improve their separations</li><li>Access to resources to support your own research to stay better informed.</li></ul><blockquote>Speaker: Kenneth Berthelette (Principal Scientist, Waters Corporation)Ken earned his Bachelor of Science in Professional Chemistry from Bridgewater State University in Bridgewater, Massachusetts in 2010.He joined Waters Corporation in March 2012, where he contributed to multiple product launches as part of the Research and Development team. In 2021, Ken transitioned into a role supporting Chemistry Aftermarket initiatives.With a strong focus on applications and customer education, he has delivered numerous seminars and webinars covering topics such as method development, method modernization and transfer, HILIC separation techniques, and LC system troubleshooting.</blockquote>

Improving Sensitivity and Throughput in Early Drug Development with Inert LC Technologies

Learn how MaxPeak Premier Columns enhance LC workflows, improving sensitivity and peak shape for acidic analytes in early drug development.

Early Drug Development with Inert LC

Compliance with IC methods US EPA 300.1 Parts A and B, ISO 10304 Parts 1 and 4, or US EPA 557 can make efficient monitoring of environmental waters a challenge. Selecting the right column technology is key, whether you are working with carbonate or hydroxide eluents.In our upcoming webinar, we focus on a new generation of high-capacity anion exchange columns. We will show how innovative column chemistry enables lowest detection limits and improved robustness against complex matrices for fully compliant analysis of environmental waters.Two complementary analytical strategies for the analysis of common anions and oxyhalides are presented in the webinar: The carbonate-based eluent approach with the new Metrosep A Supp 20 column and the hydroxide-selective approach with the Metrosep A Supp 21 column. Both strategies enable performing US EPA 300.1 Parts A and B and ISO 10304 Parts 1 and 4, respectively, in one single run.We will also show how automated hydroxide eluent production provides for highly precise and reproducible analyses with ultra‑low baselines, supporting demanding applications such as the determination of haloacetic acids (HAA) according to US EPA 557.<h4>Key learnings of this webinar</h4><ul><li>Understand how high-capacity IC columns improve robustness, accuracy, and reproducibility of your IC analysis of environmental waters</li><li>Learn how EPA 300.1 Parts A and B and ISO 10304 Parts 1 and 4, respectively, can be resolved in a single run&nbsp;</li><li>Discover the advantages of automated eluent generation combined with hydroxide-selective columns, such as the Metrosep A Supp 19 column, for haloacetic acid analysis (US EPA 557)</li><li>Explore how the right choice of columns and eluent strategies can optimize your workflows and increase laboratory efficiency and data quality</li></ul><h4>Who should attend?</h4><ul><li>Water utility operators who need robust and reliable analytical techniques to guarantee the safety of drinking water</li><li>Lab managers responsible for their lab’s compliance with the standards and regulations for the analysis of environmental waters</li><li>Scientists, researchers, and analytical method developers who need to stay up to date on state-of-the-art methods for ion chromatography</li><li>Laboratory staff and managers interested in a reliable, cost- and time-efficient analysis technique for their monitoring of environmental waters</li></ul><blockquote>Presenter: Dr. Vincent Diederich (Product Manager Ion Chromatography Columns, Metrohm International Headquarters)Vincent Diederich received his MSc in Chemical and Bioengineering at the ETH Zurich in Switzerland and stayed there for a PhD in Chemical Engineering where he gained a deep understanding of polymer reaction engineering. Since 2015, he has worked at Metrohm International Headquarters focusing on column and method development for ion chromatography, initially as a Column Production Specialist and now as Product Manager for ion chromatography columns.</blockquote>

Metrohm: Environmental waters: A new generation of high-capacity IC columns makes analysis more efficient

Environmental waters: A new generation of high-capacity IC columns makes analysis more efficient

Discover advanced IC column strategies for EPA and ISO water analysis, improving sensitivity, robustness, and efficiency in environmental monitoring workflows.

High-capacity IC columns for water analysis

Electrogenerated Chemiluminescence (ECL) has rapidly become one of the most powerful analytical techniques for applications requiring extreme sensitivity, specificity, and quantitative performance. In this webinar, we will explore what makes ECL a unique technique for sensor development providing key hints and examples.We will begin by introducing the core principles behind the ECL mechanism, including its advantages over traditional methods. We will then move on to discuss specific case studies.To bridge science with practical implementation, we will demonstrate how advanced ECL instrumentation streamlines workflows, improves sensitivity and reproducibility, and expands the range of possible applications. This includes an overview of our instrumental solutions, highlighting their ease of use, performance benefits, and integration into routine analytical environments.<h4>By the end of the session, participants will gain:</h4><ul><li>A clear understanding of the fundamentals and advantages of ECL</li><li>Insights into cutting-edge applications in different fields driving innovation in industry and academia</li><li>Practical examples showing how ECL enables highly sensitive and selective detection</li><li>A preview of instrumental platforms designed to optimize ECL performance</li><li>Guidance on choosing the right systems and configurations for specific analytical needs</li></ul>This webinar is ideal for researchers in sensor development that are looking for electrochemical techniques to improve the sensitivity on their concrete application.&nbsp;<blockquote>Presenter: Ibáñez Martínez, David (Spectroelectrochemistry Manager, Metrohm Dropsens)PhD in Chemistry (2015, University of Burgos, Spain), with expertise in the development of novel devices for time-resolved Raman spectroelectrochemistry, applied to the study of various systems such as carbon nanotubes, metal nanoparticles, conducting polymers, graphene, DNA bases, and liquid–liquid interfaces. Postdoctoral researcher (2015–2017, University of Burgos, Spain), focused on the electrochemical fabrication of Janus materials and their characterization using UV/Vis and NIR spectroelectrochemistry. Product Specialist in Spectroelectrochemistry at Metrohm DropSens S.L.U. (2018–2022) for the development of new applications and spectroelectrochemical devices. Spectroelectrochemistry Manager at Metrohm DropSens S.L.U. (2023–present), leading the development and innovation of fully-integrated spectroelectrochemical and electrochemiluminescence instrumentation.</blockquote>

Metrohm: Electrochemiluminescence: The technique that should be on your radar for sensor development

Electrochemiluminescence: The technique that should be on your radar for sensor development

In this webinar, we will explore what makes ECL a unique technique for sensor development providing key hints and examples.

Electrochemiluminescence

<h3>Bringing laboratory automation to life with Andrew+ and OneLab</h3>This webinar series explores how Andrew+ automated liquid handling and OneLab workflow software support consistent, traceable and efficient laboratory operations. Through application‑focused episodes, you will see how automation can be successfully integrated into everyday laboratory workflows, from method development to routine and regulated environments, supported by customer experiences and testimonials.<h4>Episode 1 – Proteomics Workflows</h4><h5>Overview</h5>Automation plays a key role in modern proteomics, where reproducibility and consistency are essential. In this episode, we explore how automated liquid handling supports robust, scalable sample preparation workflows while reducing manual variability across studies, with insights shared directly from proteomics laboratories using automation in practice.<h5>Key highlights</h5><ul><li>Apply automation across proteomics workflows including digestion, desalting, peptide quantification and normalization</li><li>Reduce batch effects and improve reproducibility in long-term and large-scale studies</li><li>Support evolving proteomics methods with flexible, reliable automation Speakers</li></ul><h5>Customer testimonials:</h5>Imperial College London – National Phenome Centre<ul><li>A leading UK-based academic research centre specialising in large-scale, high‑throughput proteomics and multi‑omics studies. In this episode, the team shares how automated liquid handling has been integrated into routine proteomics sample preparation workflows — from protein assays and digestion to desalting, peptide quantification and sample normalisation — enabling highly reproducible results while significantly reducing batch variation across long-term studies.</li></ul>MRC Laboratory of Medical Sciences<ul><li>A biomedical research institute focused on understanding the molecular basis of human disease, with strong expertise in proteomics and metabolomics. The laboratory provides insight into how automation supports attention‑intensive workflows such as concentration normalisation and solid phase extraction (SPE), helping to improve consistency, usability and throughput in proteomics sample preparation.</li></ul>University of Leicester – Van Geest Multiomics Facility<ul><li>A university-based multiomics facility supporting large‑scale clinical and translational research projects. In this episode, the facility explains how automated liquid handling has transformed proteomics workflows for large cohort studies, enabling scalable, flexible sample preparation while minimising batch effects and supporting highly reproducible biomarker research.</li></ul>Reserve Your Spot Today!

Transform Your Daily Lab Operations with Automation - Proteomics Workflows

Learn how automation enhances proteomics workflows, improving reproducibility, reducing batch effects, and enabling scalable sample preparation.

Transform Daily Lab Operations with Automation - Proteomics

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Regulatory expectations around nitrosamines continue to evolve, placing increasing pressure on analytical workflows to deliver both accuracy and flexibility. Join our upcoming webinar with AbbVie to see how advanced LC-MS tools can help you confidently quantify known nitrosamines and uncover unknown impurities, saving valuable time and resources.<h4>What you’ll learn:</h4><ul><li>Confident annotation &amp; accurate quantification: Leverage the SQUAD approach to precisely identify and quantify targeted nitrosamines using authentic standards and calibration curves.</li><li>Simultaneous targeted &amp; untargeted analysis: Perform comprehensive screening in a single run—detect known compounds while uncovering unexpected impurities without additional injections.</li><li>Overcoming unknown nitrosamine challenges: Optimize your LC-MS methods to improve confidence in identifying unknown nitrosamines and meet regulatory expectations.</li></ul><blockquote>Presenter: Jessica Hoskins, PhD (Principal Research Scientist I, Predictive Stability and Structure Elucidation)Jessica is a Principal Research Scientist at AbbVie where she and her team focus on Predictive Stability and Structure Elucidation to understand degradation pathways and other potential stability limiting attributes for new synthetic molecule drug substances and drug products. She has a PhD in Organic Chemistry from Tulane University with Scott Grayson, where she first became interested in mass spectrometry for polymers. Prior to joining AbbVie, she was an Adolf Martens Postdoctoral Fellow at the Federal Institute for Materials Research and Testing (BAM) in Berlin, Germany, and worked as an analytical chemist at Stepan Company in the surfactant and polymer industry.Presenter:&nbsp;Sebastien Morin (Sr Manager, Business Development, Thermo Scientific)Sebastien is a Senior Manager, Business Development at Thermo Fisher Scientific where he partners closely with Pharma and Biopharma Companies to address analytical challenges. He holds a M. SC. in Organic Chemistry from Universite de Montreal. Prior to joining Thermo Fisher Scientific, he was a Research Associate, Medicinal Chemistry for Boehringer Ingelheim, advancing virology research.</blockquote></div></div></div>

Thermo Fisher Scientific: Keeping Up with Evolving Regulatory Requirements: Addressing Analytical Challenges for Known and Unknown Nitrosamines

Keeping Up with Evolving Regulatory Requirements: Addressing Analytical Challenges for Known and Unknown Nitrosamines

Join our webinar to see how advanced LC-MS tools can help you confidently quantify known nitrosamines and uncover unknown impurities.

Analytical Challenges for Known and Unknown Nitrosamines

<h4>Key Learning Points</h4><ul><li>Understand how to prepare your LC/LC-MS system for startup with essential checks, solvent verification, and secure connections.</li><li>Follow clear, step-by-step guidance for LC startup and shutdown procedures (system agnostic guidance).</li><li>Learn best practices for properly cleaning and storing columns, both on and off the system, to maximize lifetime and performance.</li></ul><h4>Who Should Attend</h4><ul><li>The session is aimed at LC and LC‑MS users, especially those newer to the techniques, who want a clear, system‑agnostic protocol to protect columns and instruments during start‑up and shut‑down.</li></ul><blockquote>Speaker: Duilio Romanello (Senior Technical Specialist, Phenomenex)Duilio Romanello earned his MSc in Pharmaceutical Chemistry and Technology from the University of Bologna. He joined Phenomenex in 2008 as an Inside Technical Sales Consultant, later leading GC/SPE and Env/Food teams in Italy. Since 2016, he has been Account Manager for Southern Italy and GC Specialist. In 2023, he became Senior Technical Specialist for Phenomenex’s Technical team supporting scientists globally with method development, troubleshooting, and application support.</blockquote>

Phenomenex: How To Start Up and Shut Down LC and LC-MS Instruments

How To Start Up and Shut Down LC and LC-MS Instruments

Join this webinar to learn LC/LC-MS startup, shutdown, and column care best practices to improve performance, reliability, and instrument longevity.

Start Up and Shut Down LC and LC-MS Instruments

Biotherapeutic stability is essential for ensuring product quality, safety, and efficacy. In this webinar, discover how forced degradation studies combined with intact and subunit icIEF-UV/MS workflows provide deeper insights into charge variants and degradation pathways. Learn how integrating icIEF with UV and mass spectrometry enhances analytical confidence and supports robust biopharmaceutical characterization.<h4>Key highlights</h4><ul><li>Understand how the icIEF-UV/MS method works to identify charge variants in 30 minutes</li><li>Discover how it has been applied to the analysis of an intact and the sub units of a stressed monoclonal antibody</li><li>Find out how the Biologics Explorer software aids data visualisation in both intact and subunit analysis</li></ul><blockquote>Speaker: Dr. Stephen Lock, Senior Market Development Manager, SCIEX</blockquote>

SCIEX: Assessing biotherapeutic stability through forced degradation using intact and subunit icIEF-UV/MS workflows

Assessing biotherapeutic stability through forced degradation using intact and subunit icIEF-UV/MS workflows

Join this webinar to learn icIEF-UV/MS workflows for biotherapeutic stability, charge variant analysis, and monoclonal antibody characterization.

Mohlo by Vás zajímat

Webináře LabRulezLCMS týden 19/2026

Achillova pata bakterií – bez klíčového proteinu se množí pomalu

Řešení problémů se změnami retenčního času v kapalinové chromatografii