Silikagel používaný k plnění HPLC kolon je polymerem křemíku a kyslíku s povrchovými skupinami Si–OH (silanoly). Na tyto silanolové skupiny se navazují funkční skupiny pomocí silanizačního činidla za účelem funkcionalizace základního nosiče.Při funkcionalizaci HPLC kolon není možné dosáhnout 100% pokrytí fáze ani navázat všechny dostupné silanolové skupiny (volné hydroxylové skupiny na povrchu silikagelu) na stacionární fázi. Vlivem sterických překážek se s postupujícím navazováním fáze zvyšuje prostorové omezení, takže další reakce jsou obtížnější než ty předchozí.Bez end-cappingu by na koloně zůstalo značné množství přístupných, vysoce reaktivních zbytkových silanolových skupin, které mohou ovlivnit selektivitu stacionární fáze. Tyto zbytkové skupiny mohou vytvářet silné iontové a polární interakce (často označované jako „sekundární interakce“) s polárními a bazickými skupinami v analyzovaných sloučeninách. Proto je snížení počtu volných silanolů po navázání stacionární fáze pomocí end-cappingu nezbytné.Historicky se k end-cappingu používaly malé hydrofobní skupiny, povahou podobné trimethylsilanu. V posledních letech někteří výrobci zavedli další typy skupin, které dodávají koloně <a target="_blank" rel="noopener noreferrer" href="https://app.explore.phenomenex.com/e/er?s=822460770&amp;lid=6998&amp;elqTrackId=D5A4A87A868E75FA6693FC5A1E9B3C89&amp;elq=00000000000000000000000000000000&amp;elqaid=5674&amp;elqat=2&amp;elqak=8AF5A07C3CDAC28222BF387672C6F595E7A5CF7C906A90E1BF5C55FDC1EA7D8B80B0&amp;_gl=1*175w36l*_ga*MTM5ODI2NzM3NS4xNzQyMjc3MDM0*_ga_790WGKEF4G*czE3NTAwMTE2NzAkbzI1JGcwJHQxNzUwMDExNjcwJGo2MCRsMCRoMA..">nový selektivní prvek</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_The_Role_of_End_Capping_in_RP_a458911946.jpg" alt="Phenomenex: Úloha end-cappingu v reverzní fázi (RP)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_The_Role_of_End_Capping_in_RP_a458911946.jpg 245w," sizes="100vw" width="767" height="214">Pokud end-cappingové činidlo obsahuje nabité funkční skupiny, propůjčuje povrchu iontoměničovou funkci a dává koloně iontoměničové vlastnosti spolu s <a target="_blank" rel="noopener noreferrer" href="https://app.explore.phenomenex.com/e/er?s=822460770&amp;lid=8525&amp;elqTrackId=63166B02CED6893A95911CAE6AA3AE95&amp;elq=00000000000000000000000000000000&amp;elqaid=5674&amp;elqat=2&amp;elqak=8AF5B477F9C56C31BC7CAC9FBFB946361A9ECF7C906A90E1BF5C55FDC1EA7D8B80B0&amp;_gl=1*1vag7yn*_ga*MTM5ODI2NzM3NS4xNzQyMjc3MDM0*_ga_790WGKEF4G*czE3NTAwMTE2NzAkbzI1JGcwJHQxNzUwMDExNjcwJGo2MCRsMCRoMA..">reverzně-fázovými interakcemi</a> vycházejícími z C18 ligandů. Podobně polární skupiny dodávají povrchu polární charakter.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_The_Role_of_End_Capping_in_RP_4_e1b1855040.jpg" alt="Phenomenex: Úloha end-cappingu v reverzní fázi (RP)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_The_Role_of_End_Capping_in_RP_4_e1b1855040.jpg 245w," sizes="100vw" width="786" height="246">Nyní, když jsme si vysvětlili roli end-cappingu, shrňme si hlavní přínosy funkcionalizace silikagelu v závislosti na použitých chemických skupinách.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_The_Role_of_End_Capping_in_RP_3_be82757ab7.jpg" alt="Phenomenex: Úloha end-cappingu v reverzní fázi (RP)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_The_Role_of_End_Capping_in_RP_3_be82757ab7.jpg 137w," sizes="100vw" width="640" height="728">Stručně řečeno, nové technologie end-cappingu mohou významně zlepšit analýzu sloučenin, které dříve představovaly chromatografickou výzvu. Například dnes lze dosáhnout vynikajícího tvaru píků a kratších retenčních časů při analýze bazických léčiv pomocí <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=341&amp;manufacturerItems=50&amp;search=PS">kolon PS-C18</a>. Fáze <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=341&amp;manufacturerItems=50&amp;search=polar">Polar C18</a> se osvědčila pro retenci metabolitů a fáze <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=341&amp;manufacturerItems=50&amp;search=XB">XB-C18 </a>poskytuje výbornou chromatografii peptidů, s výrazně vyšší retencí malých polárních peptidů.

Úloha end-cappingu v reverzní fázi (RP)

<h4>Stanovení aniontů v N-methylpyrrolidonu (NMP) pomocí iontové chromatografie (IC)</h4>N-methylpyrrolidon (také známý jako N-methyl-2-pyrrolidon nebo NMP) je organické rozpouštědlo používané při přípravě suspenzí ve výrobě baterií a představuje klíčovou surovinu pro průmysl lithium-iontových baterií (LIB). Slouží jako účinné rozpouštědlo pro elektro­dová pojiva, například polyvinylidenfluorid, která jsou nezbytná pro zajištění stability elektrod [1,2]. NMP je během výrobního procesu zcela odstraněn a lze jej efektivně recyklovat [3]. Celosvětová poptávka po NMP je vysoká a tvoří podstatnou část výrobních nákladů lithium-iontových baterií [4].Analýza nečistot v NMP je klíčová pro posouzení kvality jak nově vyrobeného, tak recyklovaného rozpouštědla. Iontová chromatografie (IC) s eliminací matrice představuje robustní a spolehlivou techniku pro kvantitativní stanovení nečistot v NMP v rozsahu jednotek µg/L. Tato metoda umožňuje výrobcům baterií ověřit správné složení a elektrochemické vlastnosti elektrolytu a posoudit stabilitu a bezpečnost lithium-iontových baterií.Inteligentní technika předkoncentrace s eliminací matrice (MiPCT-ME) od společnosti Metrohm umožňuje kvantifikaci aniontů v N-methylpyrrolidonu až do rozsahu µg/L, a to bez nutnosti úpravy nebo ředění vzorku.<h3>VZOREK A PŘÍPRAVA VZORKU</h3>Objem 500 µl NMP byl bez jakékoli úpravy přímo dávkován do předkoncentrační kolony (PCC) IC systému pomocí jednotky 800 Dosino (807 dávkovací jednotka, 5 mL). PCC, která je instalována na místě vzorkovací smyčky, zachytává cílové ionty a umožňuje odstranění matrice. Díky tomu je možné provádět stopovou analýzu aniontů i ve složitých matricích.<h3>EXPERIMENT</h3>Analýza byla provedena na systému <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/574">930 Compact IC Flex</a> s využitím techniky MiPCT-ME a fixním dávkovaným objemem 500 µL (odpovídajícím objemu předkoncentrace). K proplachu PCC a odstranění matrice bylo použito 1,5 mL ultračisté vody (UPW). Další experimentální podrobnosti jsou uvedeny v Tabulce 1.Schéma uspořádání IC systému je znázorněno na Obrázku 1. Kalibrační rozsah byl 5–100 µg/L a standardy byly připraveny jako směsi obsahující fluorid, chlorid, dusitan, bromid, dusičnan, fosforečnan a síran. Pro zajištění porovnatelnosti byly standardy dávkovány stejným způsobem, tedy přes PCC.<h4>Tabulka 1. Parametry IC použité pro stanovení aniontových nečistot v N-methylpyrrolidonu.</h4><h5>Parametr / Nastavení</h5><ul><li>Detekce: vodivostní detekce</li><li>Kolona: Metrosep A Supp 7 - 250/4,0</li><li>Předkoncentrační kolona: Metrosep A PCC 2 HC/4,0</li><li>Objem injekce: 500 µL</li><li>Teplota: 45 °C</li><li>Eluent: 3,2 mmol/L Na₂CO₃ + 1,0 mmol/L NaHCO₃</li><li>Potlačení: sekvenční potlačení</li><li>Regenerant: 100 mmol/L H₂SO₄</li><li>Průtok: 0,7 mL/min</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_cfdb0c79ac.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: Obrázek 1. Průtokové schéma systému 930 Compact IC Flex s technikou MiPCT-ME. Předkoncentrační kolona Metrosep A PCC 2 HC/4.0 je umístěna v poloze smyčky vstřikovacího ventilu, kde slouží k zachycení analytů a odstranění matrice. Jedna jednotka Dosino zajišťuje manipulaci se vzorkem, tedy jeho transport do PCC. Druhá Dosino jednotka naplňuje proplachovací stanici čerstvou ultračistou vodou, která se používá k proplachu PCC za účelem odstranění matrice. Pro zajištění maximální čistoty ultračisté vody jsou do systému zařazeny lapače. Systém lze nakonfigurovat také tak, aby oba kroky prováděla pouze jedna jednotka Dosino. Po odstranění matrice je předkoncentrovaný vzorek přesměrován do analytické kolony a dále analyzován pomocí vodivostní detekce se sekvenčním potlačením." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_cfdb0c79ac.jpg 219w," sizes="100vw" width="1280" height="915"><h3>VÝSLEDKY</h3>Anionty byly separovány a eluovány na <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=220&amp;search=Metrosep+A+Supp+7">koloně Metrosep A Supp 7</a> za méně než 34 minut za izokratických podmínek. Naměřené koncentrace se pohybovaly v rozmezí 11–76 µg/L.Neředěný vzorek NMP byl analyzován jak bez přídavku standardu, tak s přídavkem 30 µg/L směsného standardu iontů. I u velmi nízkých koncentrací bylo dosaženo výtěžnosti v rozmezí 90–120 % (viz Tabulka 2).Obrázek 2 znázorňuje separaci aniontů v NMP. U uvedených aniontů je dosaženo separace až na základní linii. Chromatogram vykazuje dva časně eluující píky, které nebyly identifikovány. S vysokou pravděpodobností se jedná o acetát a formiát, což ukazuje značný potenciál metody pro další vývoj a rozšíření o kvantifikaci dalších relevantních aniontů.<h4>Tabulka 2. Výsledky stanovení aniontů v NMP. Vzorky byly analyzovány jak v obohacené, tak neobohacené formě a výtěžnost byla vypočtena z naměřených koncentrací.</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_2_87235d69e0.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_2_87235d69e0.jpg 245w," sizes="100vw" width="1458" height="604"><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_3_7ec62a9c74.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: Obrázek 2. Chromatogram hlavních aniontů ve vzorku NMP separovaných na koloně Metrosep A Supp 7 - 250/4.0 (karbonátový eluent) s využitím techniky MiPCT-ME pro předkoncentraci a odstranění matrice. Detekce byla provedena pomocí vodivostní detekce se sekvenčním potlačením." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_3_7ec62a9c74.jpg 245w," sizes="100vw" width="1280" height="646"><h3>ZÁVĚR</h3>Koncentrace stanovených aniontů v NMP se pohybovaly v rozmezí 11 až 76 µg/L. Takto nízké koncentrace analytů v kombinaci s rušivou matricí mohou představovat značnou výzvu pro chromatografickou analýzu.Technika Metrohm MiPCT-ME umožňuje stanovení stopových množství aniontů ve vysoce používaném rozpouštědle v procesu výroby lithiových baterií. Tato analytická metoda může významně přispět k zajištění kvality, životnosti a bezpečnosti lithium-iontových baterií.Metodu lze snadno přenést i na další relevantní rozpouštědla, jako jsou methanol, ethanol, aceton a 2-propanol.<blockquote><h3>Kontakt</h3>Metrohm Česká republika s.r.o. Na Harfě 935/5c 190 00 Praha&nbsp;<a href="office@metrohm.cz">office@metrohm.cz</a></blockquote><h3>KONFIGURACE</h3><h4>930 Compact IC Flex Oven/SeS/PP/Deg</h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/574">930 Compact IC Flex</a> Oven/SeS/PP/Deg je inteligentní kompaktní iontový chromatograf s kolonovou pecí, sekvenčním potlačením, peristaltickým čerpadlem pro regeneraci potlačovače a vestavěným odplyňovačem. Přístroj je kompatibilní s libovolnou separační a detekční metodou.Typické oblasti použití:<ul><li>Stanovení aniontů nebo kationtů se sekvenčním potlačením a vodivostní detekcí</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_4_923296ddc3.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: 930 Compact IC Flex Oven/SeS/PP/Deg." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_4_923296ddc3.jpg 156w," sizes="100vw" width="1024" height="1024"><h4>Vodivostní detektor pro IC</h4>Kompaktní, inteligentní a vysoce výkonný vodivostní detektor pro přístroje IC. Vynikající teplotní stabilita, kompletní zpracování signálu uvnitř chráněného detekčního bloku a nejnovější generace DSP (digitálního zpracování signálu) zajišťují maximální přesnost měření. Díky dynamickému pracovnímu rozsahu není nutné přepínání měřicích rozsahů – ani automatické.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_5_91b459d992.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: Vodivostní detektor pro IC." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_5_91b459d992.jpg 245w," sizes="100vw" width="1280" height="646"><h4>Profesionální procesor vzorků 858</h4>Profesionální procesor vzorků 858 umožňuje zpracování vzorků v objemu od 500 µL do 500 mL. Přenos vzorku je realizován buď pomocí peristaltického čerpadla v systému 850 Professional IC, nebo prostřednictvím jednotky 800 Dosino.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_6_6bb1a4ec02.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: Profesionální procesor vzorků 858." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_6_6bb1a4ec02.jpg 85w," sizes="100vw" width="723" height="1322"><h4>Metrosep A Supp 7 - 250/4.0</h4>Vedlejší produkty dezinfekce vody jsou podezřelé nejen z negativních účinků na zdraví, ale také z karcinogenity. Oxyhalogenidy se proto staly předmětem řady výzkumů a standardizačních norem (např. EPA 300.1 část B, EPA 317.0, EPA 326.0). Největší obavy vyvolává bromičnan, který vzniká z bromidu při ozonizaci pitné vody.Kolona Metrosep A Supp 7 - 250/4.0 je vysoce výkonná separační kolona určená pro paralelní stanovení standardních aniontů, oxyhalogenidů a kyseliny dichloroctové. Tyto ionty lze pomocí této kolony spolehlivě a přesně stanovit až na úrovni jednotek µg/L. Díky použití polymeru na bázi 5µm polyvinylalkoholu se dosahuje vysoké citlivosti detekce, což umožňuje dosažení extrémně vysokého počtu teoretických pater a vynikajících separačních vlastností. Separaci lze navíc optimalizovat úpravou teploty podle specifických požadavků aplikace.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_7_d76750b2dd.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: Metrosep A Supp 7 - 250/4.0." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_7_d76750b2dd.jpg 156w," sizes="100vw" width="1280" height="1281"><h4>Metrosep A PCC 2 HC/4.0</h4>Pro předkoncentraci aniontů a eliminaci matrice. Zvýšený objem náplně zvyšuje kapacitu obou variant předkoncentračních kolon, které jsou vyrobeny výhradně z materiálu PEEK. Vysoká kapacita je zásadní zejména tehdy, když matrice může způsobit přetížení kolony nebo při analýze vzorků s vysokou iontovou silou.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_8_50b1ca066d.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: Metrosep A PCC 2 HC/4.0." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_8_50b1ca066d.jpg 156w," sizes="100vw" width="1280" height="1280"><h4>800 Dosino</h4>800 Dosino je pohonná jednotka s funkcí čtení/zápisu pro inteligentní dávkovací jednotky. Je vybavena pevným kabelem o délce 150 cm.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_9_b17e94a7fd.jpg" alt="Metrohm: Anionty v rozpouštědlech pro lithium-iontové baterie: 800 Dosino." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Metrohm_Anionty_v_rozpoustedlech_pro_lithium_iontove_baterie_9_b17e94a7fd.jpg 245w," sizes="100vw" width="1280" height="743">

Anionty v rozpouštědlech pro lithium-iontové baterie

Vážení zákazníci, přijďte za námi na seminář: Altium žije (gen)omikou!<ul><li>Termín: 23.10.2025</li><li>Čas: 9:30 - 16:00</li><li>Místo: Hotel AVANTI, Brno</li></ul><blockquote>👉Přihlášení na seminář <a target="_blank" rel="noopener noreferrer" href="https://hpst.cz/form/altium-zije-gen-omikou">ZDE</a>Účast je bezplatná.</blockquote><h3>Hlavní témata semináře:</h3><h4>Multi-omics - technologie pro moderní výzkum</h4><ul><li>Stomics – spaciální transkriptomika (studium genové exprese v kontextu tkání)</li><li>Multiplexová fluorescenční IHC (Akoya - multiplexní zobrazování proteinů v tkáňových řezech)</li><li>Interakce nejen v proteomice (Nicoya - studium kinetických parametrů u interakcí biomolekul)</li></ul><h4>Moderní technologie pro sekvenování a molekulární diagnostiku</h4><ul><li>Rychlé, přesné a flexibilní sekvenátory MGI pro výzkum i diagnostiku (Nový vysokokapacitní sekvenátor DNBSEQ-T1+, sekvenátor DNBSEQ-G99)</li><li>Agilent Avida – nové přístupy pro cíleně nabohacené a methylační knihovny</li><li>Sistemas Genomicos – IVD certifikovaný exom a onkologické panely</li><li>Agilent SureSelect Max – nová generace library prepů pro systém SureSelect</li><li>PentaBase – pokročilé molekulárně genetické testy (q-PCR) pro klinickou diagnostiku</li><li>FemtoPulse – inovativní přístup k analýze (nejen) long-reads sekvenačních knihoven</li><li>NGS a onkogenetika v pohybu: Co je nové je v analýze somatických variant</li></ul><h4>Preimplantační a prenatální diagnostika</h4><ul><li>Rychlá PGT diagnostika na sekvenátoru DNBSEQ-G99</li><li>NIPT - Sistemas Genomicos (IVD certifikovaný test)</li></ul><blockquote>Pro více informací nás neváhejte kontaktovat na emailu:genomics_sales.cz@altium.net nebo kontaktujte přímo našeho produktového specialistu Jana Langera, tel. 739 065 914, e-mail: jan.langer@altium.net.</blockquote>

Altium žije (gen)omikou!

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Nejbližší akce

Naše společnost nabízí řešení v oblasti iontové analýzy pomocí titrace, iontové chromatografie, voltametrie a polarografie.

Metrohm Česká republika

Je autorizovaným distributorem společnosti Agilent Technologies pro Českou republiku v oblastech chromatografie (GC, HPLC, UHPLC, kapilární elektroforéza) a hmotnostní spektrometrie (GC/MS, LC/MS, CE/MS), disolučních aparátů, molekulární (UV-Vis, UV-Vis-NIR, fluorescence, FTIR analyzátory a mikroskopy) a atomové spektroskopie (ICP-OES, ICP-MS, AAS), a v neposlední řadě také v oblasti molekulární biologie a genomiky (analýza nukleových kyselin a proteinů, microarray skenery a skla, PCR & RT-PCR, qPCR, řešení pro NGS, reagencie pro mutagenezi a klonování, sondy FISH a další).

Altium International

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Separation Science

Agilent Technologies

Thermo Fisher Scientific

SCIEX

Waters Corporation

Analytical Strategies for mRNA Therapeutics and Vaccines Messenger RNA (mRNA) therapeutics and vaccines represent a groundbreaking advancement in modern medicine.Ensuring their quality, safety, and efficacy requires rigorous analytical methods focused on Critical Quality Attributes (CQAs) such as:<ul><li>mRNA integrity</li><li>Purity</li><li>Capping efficiency</li><li>Residual impurities</li></ul>To characterize and quantify these CQAs, chromatographic techniques play a central role, including:<ul><li>Ion-pair reverse-phase HPLC (IP-RP-HPLC)</li><li>Size-exclusion chromatography (SEC)</li><li>Ion-exchange chromatography (IEX)</li><li>MS Technology &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;</li></ul>Sarita and Catalin explore the importance of defining and monitoring critical quality attributes (CQAs) and outline analytical strategies for mRNA characterization and impurity profiling. Sarita emphasizes the value of reference materials in method development, bridging, and assay performance monitoring to ensure process consistency and reliability. Catalin will exemplify the use of mass spectrometry to iterate it's ability to create critical quality attributes.<h5>Key learning objectives:</h5><ul><li>Gain an overall understanding of Critical Quality Attributes for messenger RNA</li><li>Discover the current analytical strategies for creating critical quality attributes. for mRNA thru chromatography and Mass Spectrometry.</li></ul><h5>Who should attend:&nbsp;</h5><ul><li>Analytical Scientist and chemists involved in Method Development, Validation, and Quality Control of mRNA products. &nbsp; Biopharmaceutical and Biotechnology Professionals. &nbsp;Regulatory Affairs Specialists. &nbsp;Process Development Scientists.</li></ul><blockquote>Presenter: Sarita Kattel, M.S. (Principal Scientist, Global Biologics, US Pharmacopeia)Sarita Kattel serves as a Principal Scientist in USP’s Global Biologics Department. At USP, she utilizes her extensive vaccine experience to advance the development of standards supporting vaccine manufacturing. Her role also includes creating new standards for analytical testing of oligonucleotide products and exploring additional opportunities for standards in various other areas.Ms. Kattel’s career spans over biotech companies, academic institutions, contract research organizations (CROs), and pharmaceutical giants like GlaxoSmithKline (GSK) and Janssen Pharmaceuticals. She has held lab-based roles in method development and validation, Chemistry, Manufacturing, and Controls (CMC) leads, as well as quality control, working on monoclonal antibodies, proteins, and mRNA-based vaccines.Presenter:&nbsp;Catalin Doneanu PhD. (Principal Scientist, Waters Corporation)Catalin Doneanu received his Ph.D. in analytical chemistry from Oregon State University in 2002. After a postdoctoral stage in the Department of Medicinal Chemistry at University of Washington in Seattle, he joined Waters Corporation in 2006. He is currently a principal scientist in the Biopharmaceutical Sciences group. He has more than twenty years of experience in separation sciences and mass spectrometry. His current research interests are in the area of therapeutic oligonucleotides and protein characterization using LC-MS. Catalin has over 30 peer-reviewed publications.Moderated by: Samuel Seal (Biopharma Market Development Manager, Waters Corporation)Samuel Seal is a market development manager specializing in the biologics space. He loves LC and MS – especially on how this technology provides benefit and value.Sam joined Waters in January of 2014 as a Sales Technical Support Specialist in the Irvine office/lab. As a TSS he performed demonstrations on various instruments, provided training for customers, often presented on technical information at varying events, and assisted with troubleshooting from Colorado to Los Angeles.He previously worked at Pyxant Labs in Colorado Springs, CO where he gained extensive experience developing and validating methods for small/large molecules in bioanalytical assays, agricultural commodities, registration of pesticides, and worker exposure studies. He also has experience in column chromatography, sample extraction, sample clean-up, reporting and data management. Sam has hands on experience with running and maintaining various manufacturers' LC/MS/MS systems. Prior to Pyxant, Sam was a Laboratory Instructor at the University of Colorado at Colorado Springs where he earned his Bachelor of Arts in Biology and Bachelor of Arts in Chemistry.</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

mRNA Vaccines

Discover current analytical strategies for creating critical quality attributes for mRNA thru chromatography and MS.

As lab productivity demands rise, ensuring your HPLC system is prepared to perform is crucial. By understanding how the key components of your HPLC system work, you can help minimize downtime. This webinar will focus on one such critical component: the Agilent HPLC sample injector. We will explore the capabilities of both autosamplers and manual injectors, discuss best practices to maximize uptime, and cover essential maintenance to keep your injectors running smoothly day after day.<blockquote>Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.</blockquote>

Agilent Technologies: Becoming a Better Chromatographer - Educational Webinar Series

Getting the Most from Your Agilent HPLC Autosampler or Manual Injector

We will explore the capabilities of both autosamplers and manual injectors, discuss best practices to maximize uptime, and cover essential maintenance

Agilent HPLC Autosampler or Manual Injector

<h4>Key Learning Points</h4><ul><li>Recieve an overview of chromatography modes and how they works. Normal Phase, Reversed Phase, HILIC, Ion Exchange, Ion Exclusion, Size Exclusion (GPC/GFC).&nbsp;</li><li>Develop the ability to distinguish between various chromatography modes</li><li>Tune in for a refresher on the basic theory of HPLC</li></ul><h4>Who Should Attend</h4><ul><li>This webinar is designed for any new LC users, this provides a foundational understanding of HPLC, with a focus on chromatographic modes.</li></ul><blockquote>Speaker: Duilio Romanello (Senior Technical Specialist)Duilio Romanello graduated with his Bachelors of Science in Pharmaceutical Chemistry and Technology from University of Bologna. Duilio then joined Phenomenex in 2008 as an Inside Technical Sales Consultant becoming Team Leader in Italy for GC/SPE in 2010 and then for the Env/Food Team. Since 2016 he has been covering the role of Account Manager for the South of Italy working remotely and acting as GC Specialist. Since November 2023 he is Senior Technical Specialist for the Technical team at Phenomenex.</blockquote>

Phenomenex: Chromatography Modes Explained: Dive into the Modes of Chromatography!

Chromatography Modes Explained: Dive into the Modes of Chromatography!

New to LC? Learn the basics of HPLC and explore key chromatography modes like RP, NP, HILIC, IEC, IEX, and SEC in this beginner-friendly webinar.

Chromatography Modes Explained

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Mass spectrometry (MS) users need software solutions that make it easier to work with complex data while ensuring accurate peak integration and quantitation. Often this comes with limitations around data management, access, and compliance. The new Thermo Scientific™ Chromeleon™ 7.4 software solves these challenges. It will take targeted MS quantitation to the next level, controlled and accessed from anywhere, with native control for Thermo Scientific MS instruments. It provides MSn data support for wide range of applications, network scalability and resilience, and adherence to the most stringent regulatory requirements, all with a single software solution.Join our webinar to learn how Chromeleon 7.4 software provides MS labs with advanced tools for their targeted quantitation workflows by combining the powerful network and compliance capabilities of Chromeleon software with industry-leading Thermo Scientific MS instrumentation to deliver a best-in-class solution.Key Learning Points:<ul><li>How to streamline targeted MS quantitation workflows with Chromeleon 7.4 software</li><li>Ways to manage complex MS data securely, reducing training and administration effort</li><li>How to work remotely with secure access to MS data and instruments from anywhere</li></ul>Who should attend:<ul><li>Mass spectrometry users in environmental, food safety, and (bio)pharma labs</li><li>Lab managers and analysts focused on productivity and data compliance</li><li>Current Chromeleon software users looking to maximize targeted MS quantitation capabilities</li></ul><blockquote>Presenter: Richard Law (Product Manager, Thermo Fisher Scientific)With a background in analytical chemistry and mass spectrometry, Richard is the Product Manager for targeted MS using Chromeleon CDS. He has extensive experience developing applications using LC and GC–MS technologies, especially GC-triple quadrupole MS/MS and HRAM, and has worked across several industry segments.Presenter:&nbsp;Anthony Crawshaw (Product Manager, Thermo Fisher Scientific)With a background in applied chemistry, Anthony brings over 25 years of experience in pharmaceutical analysis and laboratory software. Since 2011, he's helped customers deploying Chromeleon enterprise systems, focusing on streamlining lab operations and ensuring smooth software deployments. Anthony is now the Product Manager for Chromeleon Enterprise software.Presenter:&nbsp;Laércio Fernandes (Product Marketing Manager, Thermo Fisher Scientific)</blockquote></div></div></div>

Thermo Fisher Scientific: Targeted MS quantitation - Controlled and accessed from anywhere

Targeted MS quantitation - Controlled and accessed from anywhere

Join webinar to learn how Chromeleon 7.4 software provides MS labs with advanced tools for their targeted quantitation workflows.

Targeted MS quantitation with Chromeleon 7.4 software

Join this webinar to learn from our experts about the power of capillary electrophoresis (CE) analysis and how it can provide benefit to your research. No matter your level of CE knowledge, the session will shine a light on this technique and showcase its applicability across various industries. Our speakers will outline how to develop methods for protein characterization, RNA/DNA analysis and small molecule detection and discuss some of the innovative integrated CE technologies. Using real examples we will demonstrate what is possible to achieve with the range of CE consumable kits available.<blockquote>Presenter: Dr. Sarah Meyer (Regional Service Specialist, SCIEX)Sarah earned her doctorate in Analytical Chemistry in 2022. Her research focused on bioanalytical applications, particularly investigating the effectiveness of radiotherapy in cancer treatment. She developed high-resolution separation methods and novel mass spectrometry-based detection approaches to enhance analytical sensitivity. That same year, Sarah joined SCIEX as a Regional Specialist for Capillary Electrophoresis in the Asia-Pacific region, supporting teams and customers with technical and analytical challenges.Presenter:&nbsp;Dr Charlie Liu (Field Applications Specialist, SCIEX) Charlie obtained his PhD in biopolymers in 2014. He has experience as an analytical chemist and as a chemistry department manager at Biotest Laboratories and BJP Laboratories where he gained expertise in method development and validation in analytical chemistry, especially LC-MS/MS. In 2021, Charlie joined SCIEX Oceania as a Field Application Specialist focusing on pharmaceutical, food and environmental applications.Presenter:&nbsp;Jeremy Potriquet (Field Applications Specialist, SCIEX) Jeremy has extensive knowledge of peptide and protein analysis and quantification from previous roles as a scientific officer and proteomics manager. He is experienced in processing a variety of sample matrices ranging from cancer-related matrices to those for infectious diseases covering the study of exosomes, parasites, venoms and lately immunology. Mass spectrometry has always been an essential part of &nbsp;Jeremy's career and with SCIEX as a Field Application Specialist, he applies his knowledge to provide support and guidance for the numerous SCIEX technology users in ANZ.</blockquote>

SCIEX: Capillary electrophoresis applications

Capillary electrophoresis applications

Join this webinar to learn from our experts about the power of capillary electrophoresis (CE) analysis and how it can provide benefit to your research.

Dissolution and drug release testing are used to demonstrate the consistency of pharmaceutical product performance within and across batches of critical medicines. Learn from a USP expert the importance of fully qualifying dissolution testing equipment. Understand the rationale for developing a new formulation used for the performance verification test (PVT) mentioned in USP General Chapter &lt;711&gt; Dissolution. See USP data that demonstrates the improved sensitivity of USP’s PVT reference standard—the dissolution performance verification standard (DPVS). Understand the risks of choosing an either-or approach when it comes to considering mechanical calibration and performance verification testing (PVT) for dissolution instrument qualification procedures.<h5>By attending this presentation, you will learn about:</h5><ul><li>The rigorous development of the USP Dissolution Performance Verification Standard (DPVS)</li><li>Potential sources of variability in dissolution testing related to the equipment operation and setup</li><li>Risks associated with only choosing mechanical calibration or performance verification testing in the qualification of dissolution testing equipment</li><li>What it means to have a “fully qualified” dissolution instrument according to USP specifications.</li></ul><h5>Who should attend:</h5><ul><li>Pharmaceutical industry QC analysts, lab supervisors, formulation scientists, regulatory professionals, and all those responsible for dissolution method execution, development, and compliance.</li></ul><blockquote>Presenter: Mark Liddell, PhD (Senior Manager, Dosage Form Performance Laboratory, USP)&nbsp;Dr. Mark Liddell is the Senior Manager of Dosage Form Performance Laboratory (DFPL) at USP’s Rockville headquarters. Mark has worked at USP for 18 years, pursuing and promoting a deeper understanding of the fundamentals of dissolution science. At USP Mark’s work has focused on improving the reliability and reproducibility of compendial dissolution and drug release test methods. DFPL is responsible for overseeing the development, manufacture, and monitoring of performance verification test (PVT) reference materials used to demonstrate the suitability of dissolution testing apparatuses. Mark also supports USP education course offerings, reference standard technical services, the USP Dosage Form expert committee, the USP Performance Verification Testing expert panel, and various other internal and external stakeholders as a dissolution/drug release testing subject matter expert. &nbsp;Mark’s journey spans over 25 years, dedicated to refining in vitro test methods for dosage forms and drug delivery systems.&nbsp;</blockquote>

SeparationScience: The Either-Or Trap: Consequences of Enhanced Mechanical Calibration vs Fully Qualifying your Dissolution Equipment

The Either-Or Trap: Consequences of Enhanced Mechanical Calibration vs Fully Qualifying your Dissolution Equipment

Learn from a USP expert why full qualification of dissolution equipment matters—covering DPVS development, variability sources, and compliance risks.

Mechanical Calibration vs Fully Qualifying Dissolution

<h4>Overview</h4>A comprehensive webinar on flash chromatography. The fundamentals of chromatography theory, Method development of Normal phase, TLC to Flash and diving into the specifics of reversed-phase flash materials. Exploring the power of HILIC mode for polar compounds and learn how to efficiently transfer your HPLC methods to flash chromatography for preparative purification. This webinar will equip you with practical knowledge to enhance your purification workflows.<h4>Key Learning Points</h4><ul><li>Learn about general chromatography theory</li><li>Flash Chromatography fundamentals</li><li>Method Development approach-TLC, Normal phase and Reverse phase&nbsp;</li><li>HPLC to Flash Chromatography - Know how it works.</li></ul><h4>Who Should Attend</h4><ul><li>Researchers and Scientists: Ideal for those involved in the development and characterization of biotherapeutics.</li></ul><blockquote>Speaker: Dhanunjaya Naidu (Business Development Manager, Phenomenex)Dhanunjaya Naidu has earned a master’s degree in Organic Chemistry from Andhra University, India and brings 19 years of experience in synthesis, analytical method development and Purifications. In the past 7 years at Phenomenex, he has given many technical presentations, Analytical method development support, Column positioning and troubleshooting assistance.Currently, he is working as a Business Development Manager at Phenomenex India, and responsible for Pan India Purification Technologies Products. He has publications in reputed international journals in organic synthesis.</blockquote>

Phenomenex:  Enhancing Productivity Using Flash Chromatography: Strategies and Techniques

 Enhancing Productivity Using Flash Chromatography: Strategies and Techniques

Master flash chromatography fundamentals, method development, and HPLC-to-flash transfer to improve purification workflows for polar and complex compounds.

Flash Chromatography: Strategies and Techniques

The rise of therapeutics and vaccines based on large RNA structures (such as sgRNA/CRISPR and mRNA), has highlighted the need for precise RNA sequence and modification analysis. Mass spectrometry offers powerful capabilities for RNA characterization, but data interpretation can be complex and resource intensive.In this webinar, we’ll explore how Waters and MKI support RNA analysis with a comprehensive suite of tools—including novel sample prep enzymes, high-purity reagents, application-specific standards, and streamlined informatics. These innovations enable routine, in-depth characterization of critical quality attributes such as 5’ capping efficiency, 3’ poly(A) tail heterogeneity, and oligonucleotide mapping.We’ll showcase the waters_connect platform, simplifying automated MS1 data analysis with seamless integration with third-party tools. Special guest Matsubara-san from MKI will present the AQXeNA software, offering automated MS2-based oligonucleotide mapping for early-stage RNA product development<blockquote>Presenter: Yuki Matsubara (Software Scientist, Mitsui Knowledge Industry Co., Ltd.,)Transitioned from a wet lab research background to dry lab software development. Currently at Mitsui Knowledge Industry Co., Ltd., leading the development of AQXeNA, a mass spectrometry data analysis software for nucleic acids.Presenter:&nbsp;Catalin Doneanu PhD. (Principal Chemist, Waters Corporation)Catalin Doneanu received his Ph.D. in analytical chemistry from Oregon State University in 2002. After a postdoctoral stage in the Department of Medicinal Chemistry at University of Washington in Seattle, he joined Waters Corporation in 2006. He is currently a Principal Chemist in the Biopharmaceutical Sciences group. He has more than twenty years of experience in separation sciences and mass spectrometry. His areas of expertise in LC-MS include analysis of oligonucleotides, host cell protein analysis, MS based quantification of peptides and proteins, protein characterization and analysis or protein aggregates. Catalin has over 30 peer-reviewed publications.</blockquote>

Waters Corporation: Expanding the mass spectrometry and informatics toolsets for comprehensive analysis of large RNA constructs

Expanding the mass spectrometry and informatics toolsets for comprehensive analysis of large RNA constructs

Discover tools and workflows for mass spectrometry-based analysis of large RNA, from 5' capping to MS2 mapping, using Waters and MKI innovations.

Mass Spec & Informatics for Large RNA Analysis

In fast-paced environments like research, development, or manufacturing, providing timely and accurate information is crucial for achieving success amidst growing sample numbers and complexity.The Agilent InfinityLab Pro iQ Series is the game-changing next generation of LC-mass detection. These systems are built to effortlessly manage diverse analytical needs with confidence, ensuring you are ready to ride the wave of innovation with ease. Advance your lab with the performance and intelligence of next-generation LC-mass detection—Agilent InfinityLab Pro iQ Series.<h5>What you will learn about</h5><ul><li>The value of mass detection to accelerate development of therapeutic modalities large and small</li><li>Innovative performance to purify the smallest quantities or detect the lowest impurities &nbsp;</li><li>Instrument intelligence that enables rapid deployment and operation as easy as UV</li><li>Streamlined workflows for mass confirmation, mass-based purification, and impurity detection</li></ul><h5>Who should attend</h5><ul><li>Analytical R&amp;D Directors, Lab Managers and Scientists supporting pharma and biopharma drug discovery, development, and manufacturing</li><li>Lab Manager, Researchers and Scientists requiring fast affordable mass confirmation, quantitation, and screening workflows to support research and development in food, environmental, chemical materials, and life sciences&nbsp;</li></ul><blockquote>Presenter: Russell Burge (LC/SQ and LC/TOF Technical Product Manager, Agilent Technologies, Inc.)Russell earned a PhD in biochemistry and molecular biology from Scripps Research. He has extensive experience in the R&amp;D of biomolecules and biopharmaceuticals, and the implementation of automated workflows. Russell has acted as a Product Manager for various products in Life Science and Industrial Markets, and he currently manages the LC/MS SQ and LC/MS TOF product portfolios at Agilent.Presenter: Ian Edwards (Strategic Marketing Product Manager (SQ/TQ), Agilent Technologies, Inc.)Ian holds a Ph.D. from the Centre for Proteome Research at the University of Liverpool. He has spent time as a pre- and post-sales MS applications specialist focused on MS imaging and proteomics, as a field marketing manager for -omics, and as a global pharmaceutical marketing manager. Currently Ian manages the strategic product marketing for LC/MS SQ and LC/MS Triple Quadrupole product portfolios at Agilent.</blockquote>

Agilent Technologies: It's not the analytes you see. It's the ones you miss that count. Unveiling Next-Gen LC-Mass Detection

It's not the analytes you see. It's the ones you miss that count. Unveiling Next-Gen LC-Mass Detection

Unlock faster, smarter LC-mass detection with the Agilent InfinityLab Pro iQ Series—built for efficient workflows in pharma, biotech, and beyond.

Unveiling Next-Gen LC-Mass Detection

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div>Mineral Oil Hydrocarbons (MOH) are increasingly recognized as a significant concern in food safety, driving demand in the food testing market globally and particularly in India. The complexity of analyzing Mineral Oil Saturated Hydrocarbons (MOSH) and Mineral Oil Aromatic Hydrocarbons (MOAH) necessitates meticulous optimization at every stage of the analytical process.Join our upcoming webinar, "MOSH MOAH Analysis Made Clear: Strategies for Diverse Matrices," to learn from our experts about advanced MOSH/MOAH analysis techniques designed to tackle diverse matrices with exceptional efficiency and precision. Discover how cutting-edge hyphenated techniques like LC-GC, LC-GC × GCMS, and GC × GC-MS are revolutionizing the automation and accuracy of MOSH/MOAH analysis. These methods are adept at handling complex matrices, ensuring reliable results across various applications.To further enrich your learning experience, we will conclude the webinar with a live Q&amp;A session, providing an opportunity for you to engage directly with our experts and get answers to your specific questions<blockquote>Presenter: Jorn Hofsteenge (Analytical Support Specialist, Interscience Netherlands)Jorn Hofsteenge studied analytical chemistry at the Avans University of Applied Sciences in the Netherlands. He began his career in the QC department of a pharmaceutical company, where he worked for two years before joining Interscience seven years ago. For the past six years, he has been primarily responsible for the MOSH/MOAH application. Since then, both the system and the team have grown. Jorn is now part of the Interscience MOSH/MOAH team, which consists of four specialists. His focus is mainly on the development of the system, training of technicians and customers, and performing installations of the system around the world.Presenter:&nbsp;Inge de Dobbeleer (Account Manager, Interscience Netherlands)Inge de Dobbeleer holds a bachelor's degree in Biochemistry. She began her career at the Belgian Food Safety Authority, then moved to the Netherlands to develop environmental analysis methods at SGS in Rotterdam. She later joined Interscience, installing GCMS systems across the country. In her forties, Inge became EU GCMS expert at Thermo Fisher Scientific, eventually overseeing EMEA GCMS marketing. Today, she is part of Interscience’s commercial team, working closely with Thermo Fisher on specialized GC and GCMS solutions.</blockquote></div></div></div>

Thermo Fisher Scientific: MOSH MOAH Analysis Made Clear: Strategies for Diverse Matrices

MOSH MOAH Analysis Made Clear: Strategies for Diverse Matrices

Join our webinar to learn about advanced MOSH/MOAH analysis techniques designed to tackle diverse matrices with exceptional efficiency and precision.

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