<p><strong>Tento návod vysvětluje výběr vhodné kolony pro size exclusion chromatography (SEC) při analýze biologických molekul. SEC separuje molekuly na základě jejich hydrodynamického poloměru, tedy podle velikosti a tvaru, nikoliv podle molekulové hmotnosti.</strong></p><ul><li>Molekuly pronikají do pórů stacionární fáze</li><li>Nedochází k chemickým interakcím</li><li>Větší molekuly eluují rychleji</li><li>Menší molekuly eluují později</li></ul><h3><span><strong>Princip separace v SEC</strong></span></h3><ul><li><strong>Velké molekuly</strong><br>→ nevstupují do pórů<br>→ pohybují se pouze mezi částicemi (intersticiální prostor)<br>→ eluují dříve</li><li><strong>Malé molekuly</strong><br>→ vstupují do pórů<br>→ mají delší dráhu<br>→ eluují později</li></ul><h3><span><strong>Klíčové parametry pro výběr kolony</strong></span></h3><h4><span><strong>1. Charakter molekuly</strong></span></h4><p><strong>Zohledňuje se:</strong></p><ul><li>hydrodynamický poloměr</li><li>tvar molekuly</li></ul><p><strong>Příklady:</strong></p><ul><li><strong>peptidy: </strong>~0,7 nm</li><li><strong>AAV: </strong>~25 nm</li><li><strong>mRNA: </strong>protáhlý, neglobulární tvar</li></ul><h4><span><strong>2. Velikost pórů</strong></span></h4><ul><li>Optimální velikost:<br><strong>3–4× větší než poloměr analyzované molekuly</strong></li></ul><h4><span><strong>3. Velikost částic</strong></span></h4><ul><li>≤ 3 µm</li><li>zajišťuje vysokou účinnost separace</li></ul><h4><span><strong>4. Materiál stacionární fáze</strong></span></h4><ul><li>Preferována <strong>mechanicky odolná silika</strong></li><li>Odolnost vůči zpětnému tlaku</li></ul><h4><span><strong>5. Povrchová chemie</strong></span></h4><ul><li><strong>Chemická inertnost povrchu je zásadní</strong></li><li>Preferována je <strong>diolová chemie</strong>, protože:<ul><li>minimalizuje interakce s analytem</li><li>poskytuje <strong>vysoce hydrofilní povrch</strong></li></ul></li></ul><h4><span><strong>6. Konstrukce kolony</strong></span></h4><ul><li><strong>bioinertní provedení</strong></li><li>zajišťuje:<ul><li>minimální ztráty analytu</li><li>vysokou reprodukovatelnost</li></ul></li></ul><h3><span><strong>Dostupné SEC kolony (</strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=353&amp;manufacturerItems=50&amp;search=dsec"><span><strong>řada BioZen dSEC</strong></span></a><span><strong>)</strong></span></h3><h4><span><strong>1. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/2192"><span><strong>90 Å (9 nm)</strong></span></a></h4><ul><li>peptidy</li><li>malé proteiny</li><li>agregáty do ~50 kDa</li></ul><h4><span><strong>2. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/1534"><span><strong>200 Å</strong></span></a></h4><ul><li>protilátky</li><li>konjugáty protilátka-léčivo</li><li>krátké oligonukleotidy</li></ul><h4><span><strong>3. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/1535"><span><strong>700 Å (70 nm)</strong></span></a></h4><ul><li>AAV</li><li>mRNA</li><li>velké proteinové komplexy (např. IgM)</li></ul><h3><span><strong>Charakteristiky kolon</strong></span></h3><ul><li>siliková matrice</li><li>diolová chemie (hydrofilní, inertní)</li><li>bioinertní hardware</li><li>vysoká výtěžnost a reprodukovatelnost</li></ul><h3><span><strong>Výkon a kalibrace</strong></span></h3><h4><span><strong>Proteiny a AAV</strong></span></h4><ul><li><strong>90 Å</strong> → nízké molekulové hmotnosti (≤ 50 kDa)</li><li><strong>200 Å</strong> → střední rozsah (do ~450–500 kDa)</li><li><strong>700 Å</strong> → velké molekuly (včetně AAV)</li></ul><h4><span><strong>RNA a DNA</strong></span></h4><ul><li>separace molekul s neglobulárním tvarem</li><li><strong>200 Å</strong> → menší oligonukleotidy</li><li><strong>700 Å</strong> → dlouhé řetězce (až ~3000–4000 nukleotidů)</li></ul><h3><span><strong>Závěr</strong></span></h3><p><strong>Správný výběr SEC kolony vyžaduje:</strong></p><ul><li>znalost velikosti a tvaru molekuly</li><li>odpovídající velikost pórů</li><li>vhodnou velikost částic</li><li>inertní chemii a konstrukci</li></ul><p>To umožňuje dosáhnout optimální separace biologických molekul.</p>

Jak vybrat správnou SEC kolonu pro analýzu biologických molekul

<p><strong>Zveme vás na odborný seminář zaměřený na moderní svět separačních technik a hmotnostní spektrometrie. Seminář se bude konat 29. dubna 2026 od 13:00 hodin v Aule Arnošta z Pardubic (Studentská 519, Pardubice).</strong></p><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://forms.office.com/Pages/ResponsePage.aspx?id=aR-wpy9pT0iMxW5H7RJK8WO2jiQLb7NOszjVkgYyezJUMFNTVktGUUZCTUIzMUwzMUM5TDM5QTFLUS4u"><strong>Registrace</strong></a></p><p><i>Kapacita je omezená, žádáme Vás o registraci</i></p><p><i><strong>Občerstvení bude zajištěno.</strong></i></p></blockquote><h3><strong>Program</strong></h3><h5><strong>13:00 Vítejte!</strong></h5><ul><li>Lukáš Plaček</li></ul><h5><strong>Nová generace HPLC&nbsp;</strong></h5><ul><li>Zuzana Snížková</li></ul><h5><strong>Plynová a iontová chromatografie v aplikacích&nbsp;</strong></h5><ul><li>Magdalena Voldřichová</li></ul><h5><strong>Síla LC-MS s jednotkovým rozlišením&nbsp;</strong></h5><ul><li>František Zaleš</li></ul><h5><strong>Potřeba vysokého rozlišení v LC-MS</strong></h5><ul><li>Roman Hájek</li></ul><h5><strong>14:15 Diskuze a zakončení</strong></h5><p>&nbsp;</p><p><strong>Těšíme se na Vás.</strong></p><p><strong>Váš tým Pragolab s.r.o.</strong></p>

Pozvánka na Pragolab seminář Moderní svět separačních technik a hmotnostní spektrometrie

<p><strong>HALO® BIOCLASS Oligonucleotide kolony posouvají analýzu oligonukleotidů na novou úroveň. Využívají osvědčenou Fused-Core® technologii společnosti AMT, která zajišťuje vysoce efektivní a rychlé separace optimalizované přímo pro potřeby oligo analýzy. Nejnovější přírůstek do portfolia – kolona OLIGO 1000Å – přináší ještě lepší výkon při separaci větších a strukturálně složitějších oligonukleotidů.</strong></p><p>Díky tomu HALO® nabízí kompletní OLIGO řešení pokrývající široké spektrum separačních výzev.</p><ul><li><strong>P2762-102: </strong>HALO 1000 Å OLIGO C18 , 2.7 µm, 2.1 x 5 mm, 3 pk.</li><li><strong>P2762-302: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 30 mm - INERT</li><li><strong>P2762-402: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 50 mm - INERT</li><li><strong>P2762-602: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 100 mm - INERT</li><li><strong>P2762-702: </strong>HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 150 mm - INERT</li><li><strong>P2762-902:</strong> HALO 1000 Å OLIGO C18, 2.7 µm, 2.1 x 250 mm - INERT</li><li><strong>P2A62-402:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 2.1 x 50 mm</li><li><strong>P2A62-602:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 2.1 x 100 mm</li><li><strong>P2A62-702: </strong>HALO 120 Å OLIGO C18, 2.7 µm, 2.1 x 150 mm</li><li><strong>P2A64-402: </strong>HALO 120 Å OLIGO C18, 2.7 µm, 4.6 x 50 mm</li><li><strong>P2A64-602:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 4.6 x 100 mm</li><li><strong>P2A64-702:</strong> HALO 120 Å OLIGO C18, 2.7 µm, 4.6 x 150 mm</li></ul><blockquote><p>Více informací najdete na stránce <a target="_blank" rel="noopener noreferrer" href="https://halocolumns.com/solutions/biopharma-solutions/oligonucleotide-solutions/"><strong>OLIGO Solutions | HALO®</strong></a>.</p></blockquote><h4><strong>Kolony HALO® BIOCLASS OLIGO NABÍZEJÍ:</strong></h4><ul><li><strong>Velikost pórů 120 Å</strong> – vhodné pro oligomery až do délky cca 60 bází</li><li><strong>Velikost pórů 1000 Å</strong> – pro vysoce účinnou separaci oligomerů o délce 100 a více bází</li><li><strong>Vysokou stabilitu při vysokém pH a zvýšené teplotě</strong></li><li><strong>Stacionární fázi kompatibilní s UHPLC a hmotnostní spektrometrií</strong></li><li><strong>Hardwarově pasivovaný povrch kolony</strong>, který snižuje adsorpci oligonukleotidů</li></ul><h4><strong>Brožury</strong></h4><ul><li><strong>Kolony HALO Oligo C18</strong></li><li><strong>Kolony HALO 1000 Å OLIGO C18</strong></li></ul>

HALO® BIOCLASS Oligonucleotide kolony

Akademie

Video

Článek

Produkt

Nejbližší akce

Je autorizovaným distributorem společnosti Agilent Technologies pro Českou republiku v oblastech chromatografie (GC, HPLC, UHPLC, kapilární elektroforéza) a hmotnostní spektrometrie (GC/MS, LC/MS, CE/MS), disolučních aparátů, molekulární (UV-Vis, UV-Vis-NIR, fluorescence, FTIR analyzátory a mikroskopy) a atomové spektroskopie (ICP-OES, ICP-MS, AAS), a v neposlední řadě také v oblasti molekulární biologie a genomiky (analýza nukleových kyselin a proteinů, microarray skenery a skla, PCR & RT-PCR, qPCR, řešení pro NGS, reagencie pro mutagenezi a klonování, sondy FISH a další).

Altium International

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Jsme jedním z lídrů na českém a slovenském trhu v oblasti laboratorních zařízení a svým zákazníkům přinášíme špičkové přístroje z oboru analytické chemie a fyzikálních měření více než 25 let.

Pragolab

Tosoh Bioscience

Mettler-Toledo

Bruker

Thermo Fisher Scientific

Shimadzu Corporation

Evosep Biosystems

Waters Corporation

<div><p><strong>As demand for glucagon-like peptide-1 (GLP-1) drugs continues to grow, ensuring these products meet regulatory standards while delivering fast, reliable results is critical. Traditional amino acid analysis (AAA) workflows offer valuable quantitative insights into bioprocess conditions during production but are often time-consuming and complex.</strong></p><p>In this webinar we will introduce a streamlined, microwave-assisted UPLC approach designed to rapidly hydrolyze, label, and identify GLP-1 analogs using the Waters AccQ·Tag Ultra Chemistry Kit. Discover how this turnkey solution expedites amino acid analysis while maintaining accuracy and reliability in conjunction with Empower™ Chromatography Data System (CDS) workflows.</p><h4><strong>Why this webinar matters</strong></h4><ul><li>Learn how to accurately assess GLP- 1 analog composition and quantification using AAA workflows.</li><li>Understand the benefits of rapid derivatization and the simplicity of AccQ·Tag Ultra Chemistry Kits.</li><li>Discover how Empower™ Chromatography Data System (CDS) minimizes errors through automated workflows and custom fields</li></ul><p>This webinar is designed for scientists and managers in Quality Control who work with the analysis or testing of critical quality attributes (CQAs) of peptides, proteins, and especially GLP‑1 analogs.</p><p><i>Don't miss it.</i></p><blockquote><p><strong>Presenter:</strong> Duanduan Han, Ph.D. (Senior Scientist, Waters Corporation)</p><p>Duanduan Han, Ph.D., is a Senior Scientist at Waters Corporation in Milford, MA. Her work has focused on LC/MS method development and troubleshooting, impurity profiling, raw material screening of GLP-1s, lipid nanoparticles, surfactants, oligonucleotides, mAbs, and other biologic drug products.</p><p>She received her Ph.D. in chemical engineering from Texas A&amp;M University.</p><p><strong>Presenter:</strong> Tim Anderson, Ph.D. (Senior Product Manager, Waters Corporation)</p><p>Tim Anderson, Ph.D., has received advanced degrees in Analytical Chemistry and Food Science related to extraction and MS analysis of proteins at Iowa State University. He has industry experience with large manufacturing product analysis and fit-for-use testing.</p><p>Tim has been at Waters working with consumables for 3 years and is Senior Product Manager for AccQ·Tag Ultra solutions.</p></blockquote></div>

Rapid Analysis of GLP-1 Analogs Using Pre-Column Derivatization

Discover microwave-assisted UPLC amino acid analysis workflow for accurate GLP-1 analog characterization using AccQ·Tag Ultra chemistry and Empower CDS.

GLP-1 Analogs analyis by Pre-Column Derivatization

<div><p><strong>Join us for an exclusive webinar featuring two global pioneers in spatial chemical analysis: Jonathan Sweedler from the University of Illinois Urbana-Champaign and Carsten Hopf from the Center for Mass Spectrometry and Optical Spectroscopy (CeMOS) at Mannheim University.</strong></p><h4><strong>What you will learn:</strong></h4><ul><li>How advanced multimodal imaging workflows enable highly specific ROI-focused molecular imaging in complex tissues.</li><li>How combining QCL-based IR microscopy, fluorescence microscopy and MALDI Imaging allows rapid feature detection, and deeper molecular analysis.</li></ul><h4><strong>Who should join:</strong></h4><ul><li>Experienced MALDI Imaging users aiming to improve spatial specificity, molecular identification confidence, and sample throughput.</li><li>Scientists and researchers interested in multimodal imaging workflows that integrate IR and fluorescence microscopy to guide targeted MALDI Imaging.</li></ul><h4><strong>Topics covered:</strong></h4><ul><li>New MALDI Imaging strategies for ROI-specific molecular analysis.</li><li>Multimodal integration for rapid tissue screening and biological feature labeling.</li></ul><blockquote><p><strong>Presenter:</strong> Carsten Hopf, Ph.D.</p><p>Carsten Hopf is a Professor of Bioanalytics and Drug Discovery at Mannheim University of Applied Sciences and the Director of the Center for Mass Spectrometry and Optical Spectroscopy (CeMOS). A leader in MALDI Imaging, his work focuses on advancing various imaging modalities to map the spatial distribution of drugs, lipids, and proteins in tissues and cells for complex diseases, such as cancer and Alzheimer's.&nbsp;</p><p><strong>Presenter:</strong> Jonathan Sweedler, Ph.D.</p><p>Jonathan Sweedler holds the Chair in Chemistry at the University of Illinois Urbana-Champaign and is the Editor-in-Chief of Analytical Chemistry. He's a pioneer in analytical neurochemistry, focusing his research on developing ultrasensitive mass spectrometry and imaging tools to uncover the chemical secrets of individual cells and complex tissues.&nbsp;</p></blockquote></div>

Bruker: MALDI Imaging Becomes IR-resistible: Going Multimodal for Advanced Biomedical Insights

MALDI Imaging Becomes IR-resistible: Going Multimodal for Advanced Biomedical Insights

Webinar on multimodal imaging combining MALDI, IR, and fluorescence microscopy for precise ROI-based molecular analysis in tissues.

MALDI Imaging Becomes IR-resistible

<h4><strong>Scalable workflows are no longer a nice‑to‑have in proteomics, they’re essential.</strong></h4><p>In this upcoming webinar series, we explore how standardized, automated proteomics workflows are enabling reproducible results at scale—from discovery to translational and applied research. Hear directly from experts and users as they share practical insights on building robust, high‑throughput workflows that keep pace with growing sample volumes, increasing complexity, and real‑world laboratory demands.</p><p>Join us to learn how scalable proteomics workflows are moving from promise to practice.</p><h5><strong>Automated Mag-Net Enrichment Unlocks Deep and Cost-Effective LC-MS Plasma Proteomics</strong></h5><ul><li>Salla Keskitalo, PhD, LSRI Director Viikki Proteomics Unit, University of Helsinki</li></ul><p>Plasma is an ideal material for proteomics due to its diverse protein content reflecting physiological and pathological states, and its compatibility with minimally invasive sampling. Deep proteomic profiling of plasma is limited by high-abundant proteins that mask the detection of low-abundant proteins. To overcome this, we compared five plasma protein enrichment methods, Mag-Net, ENRICHplus, ENRICHiST, EasySep, and EXONET, against neat plasma using LC-MS proteomics. All five methods substantially increased protein identifications, with Mag-Net, ENRICHplus, EasySep, and EXONET yielding up to 4200 proteins per sample, over 7-fold more than neat plasma, using a 44-minute gradient on the Evosep One and data-independent acquisition on the timsTOF Pro 2. These methods enriched extracellular vesicle-associated proteins while effectively depleting high-abundant proteins. To further enhance performance and scalability, we optimized the Mag-Net protocol by increasing the plasma-to-bead ratio and automated the workflow, including Evotip loading, on the Biomek i5 liquid handler. The automated Mag-Net, combined with the Orbitrap Astral mass spectrometer, yielded up to 4500 proteins per sample with a throughput of 100 samples per day. The automated Mag-Net enrichment strategy enables affordable, scalable, high-throughput LC-MS plasma proteomics, supporting biomarker discovery across large cohorts.</p><h5><strong>Topic to be announced</strong></h5><ul><li>Joachim Smollich, PhD Student, Faculty of Biology, Department of Molecular Cell Biology, Weizmann institute of science</li></ul>

Evosep: Scalable Workflows for standardized proteomics

Scalable Workflows for standardized proteomics

Join us to learn how scalable proteomics workflows are moving from promise to practice.

<h3><strong>Demonstration of DirectInject-LC and iC LC</strong></h3><ul><li><strong>DirectInject-LC technology overview</strong></li><li><strong>How to run an experiment with DirectInject-LC</strong></li><li><strong>Transforming HPLC into online PAT</strong></li></ul><p>Calum Welsh of METTLER TOLEDO provides an overview of DirectInject-LC™ - how it works and how to run an experiment. DirectInject-LC™ transforms HPLC into an online technique for reaction analysis that enables scientists to gain the data required to understand complex processes in near real-time.</p><blockquote><p><strong>Presenter:</strong> Calum Welsh (Reaction Analysis Group, METTLER TOLEDO)</p><p>Calum Welsh is an accomplished professional with extensive experience in the field of chemistry and product management. Currently serving as a Product Manager for Reaction Analysis at Mettler-Toledo International, Inc. since July 2019, Calum has also held the role of Technology and Application Consultant for AutoChem. Calum completed his Ph.D. and postdoctoral fellowship at The University of Nottingham.</p></blockquote>

Mettler Toledo: Online HPLC Analysis with DirectInject-LC

Online HPLC Analysis with DirectInject-LC

DirectInject-LC turns HPLC into online PAT for real-time reaction monitoring and process understanding.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Struggling to keep up with tightening PFAS regulations and shrinking reporting limits? Laboratories are under increasing pressure to deliver faster, more accurate results while managing complex environmental samples. In this Digital Classrooms session, explore a comprehensive approach to PFAS analysis that combines Shimadzu's total solution with an innovative strategy to tackle these challenges effectively.</strong></p><p>This session covers Shimadzu's PFAS capabilities across its analytical platforms — delivering precise quantification and broad-spectrum characterization across different PFAS classes, while MALDI-TOF imaging accelerates rapid screening, allowing labs to triage high-risk samples in minutes while protecting critical LC-MS/MS systems from contamination.</p><p>Whether you work in environmental testing, regulatory compliance, water quality, or research, this session provides actionable insights to improve your PFAS testing capabilities. Join us to stay ahead in the ever-evolving world of PFAS analysis. Register Now!</p><h4><strong>Learning Objectives</strong></h4><ul><li>Understand how advanced LC-MS/MS workflows enable sensitive and selective quantification of regulated PFAS compounds in environmental matrices, including strategies to meet tightening reporting limits and evolving regulatory requirements.</li><li>Recognize the value of rapid MALDI-TOF imaging as a high-throughput screening tool for identifying elevated PFAS levels in water samples, enabling efficient sample triage and reducing contamination risk to LC-MS/MS systems.</li><li>Evaluate how integrating screening and confirmatory mass spectrometry approaches improves laboratory efficiency, data confidence, and regulatory readiness in modern PFAS monitoring programs.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Ruth Marfil-Vega (Manager, Sales Headquarters, Global Co-Creation Sales Strategy Office, Shimadzu Corporation)</p><p><strong>Presenter: </strong>Caroline Jones (Global MALDI Applications Specialist, Kratos Analytical, Shimadzu Corporation)</p></blockquote></div></div></div>

Shimadzu: Environmental PFAS Testing: Introducing Next-Generation Mass Spectrometry Solutions

Environmental PFAS Testing: Introducing Next-Generation Mass Spectrometry Solutions

Explore advanced PFAS analysis strategies combining LC-MS/MS and MALDI-TOF screening.

PFAS Testing: Next-Generation Mass Spectrometry Solutions

<h4><strong>Join us for a free webinar on Analysis of Inositol Stereoisomer Profiling in Dietary Supplements and Foods</strong></h4><p>Inositol plays a key role in regulating numerous metabolic pathways and hormonal signaling processes. Maintaining a balanced profile of inositol stereoisomers in the human body is critical for supporting key biological functions and may help reduce the risk of certain health disorders. The aim of this webinar is to present analytical procedures capable of simultaneously separating and quantifying free inositol stereoisomers in both simple matrices, such as dietary supplements, and complex matrices, such as milk, cereal, and infant formula. An existing Hydrophilic Interaction Liquid Chromatography (HILIC) protocol was systematically optimized to achieve adequate resolution and reliable separation of seven inositol stereoisomers (allo-, muco-, epi-, D-chiro-, neo-, myo-, and scyllo-inositol) using a UPLC™ BEH™ Amide Column. Two different sample preparation procedures and two mass spectrometry (MS) techniques (ACQUITY™ QDa™ II Mass Detector and Xevo™ TQ-S cronos Mass Spectrometer) were developed for the analysis of the simple and complex matrices, respectively. Details of method development and analytical performance will be discussed.</p><blockquote><p><strong>Presenter: </strong>Jinchuan Yang, Ph.D. (Principal Scientist, Waters Corporation)</p><p>Dr. Jinchuan Yang is a Principal Scientist Waters Corporation, and a subject matter expert in food and environmental applications, polymer chromatography, and LC and MS technologies. Since he joined Waters in 2002, his roles at Waters have mainly focused on application development, market development, and support for R&amp;D and training. He is an active participant in technical stakeholder groups at AOAC International and USP, serving as an expert panel member in AOAC SPIFAN and participating in review, recommendation and muti-lab testing of AOAC methods.</p><p><strong>Presenter:&nbsp;</strong>Dimple Shah (Moderator | Principal Food and Materials Market Development Manager, Waters Corporation)</p><p>Dimple Shah has a M.S. degree in Organic Chemistry from St. Xavier’s college, Gujarat University, India. She also has a M.S. degree in Chemistry from University of Massachusetts at Lowell. She is working at Waters Corporation, Milford as a Principal Market Development Manager for Food and Material Science. In her previous journey she was a Principal Scientist at Waters and worked in Genotoxic impurity and Food and Environment market. She was responsible for development of analytical methods for contaminants in food and water using LC and Mass spectrometers. Her areas of expertise are LC, tandem quadruple, high resolution mass spectrometry and various chromatographic and mass spectrometry software. She has published several application notes on polar pesticides, pesticides, veterinary drugs, mycotoxins, sugars, sweeteners, vitamins, dyes and NDSRIs.</p></blockquote>

Waters Corporation: Fit-for-purpose workflows for inositol stereoisomer profiling in dietary supplements and foods using single and tandem mass spectrometry

Fit-for-purpose workflows for inositol stereoisomer profiling in dietary supplements and foods using single and tandem mass spectrometry

Analytical procedures capable of simultaneously separating and quantifying free inositol stereoisomers in simple and complex matrices.

Inositol Profiling by Single & Tandem Mass Spectrometry

<p><strong>On March 31, Jennifer Knister, Commercial Product Manager - Process Solutions at Tosoh Bioscience, will discuss the importance of seamlessly scaling up downstream bioprocessing purification efforts from PD to GMP production using pre-packed chromatography columns. Participants will learn about the impact of multi-column chromatography (MCC) on column selection, along with the economics of pre-packed versus self-packed columns in MCC processes at production scale.</strong></p><p>This webinar will introduce SkillPak™ MAX pre-packed columns and present performance data using TOYOPEARL® Super A, a novel Protein A resin.</p><p><strong>After the webinar, ask your questions during the live Q&amp;A session.&nbsp;</strong></p><blockquote><p><strong>Presenter: </strong>Jennifer Knister (Commercial Product Manager - Process Solutions at Tosoh Bioscience)</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Scaling Up from Process Development to GMP Production with Pre-packed Columns

Tosoh Tuesday - Scaling Up from Process Development to GMP Production with Pre-packed Columns

This webinar will introduce SkillPak™ MAX pre-packed columns and present performance data using TOYOPEARL® Super A, a novel Protein A resin.

From Process Development to GMP Production

<h5><strong>Key Learning Points</strong></h5><ul><li>Discover how to properly use and maintain your column to maximize its performance and lifespan.</li><li>Understand the physicochemical interactions that take place in chromatography.&nbsp;</li><li>Learn how to select columns and method conditions step-by-step.</li></ul><h5><strong>Who Should Attend</strong></h5><ul><li>Any liquid chromatography users who are looking for tips and best practices on developing proper methods and preventing troubleshooting.<strong>&nbsp;</strong></li></ul><blockquote><p><strong>Presenter: </strong>Koen Askamp (Senior Technical Specialist, Phenomenex)</p><p>Koen Askamp is a Senior Technical Specialist in Phenomenex. He was awarded his BSc and MSc degrees in Molecular Life Sciences at Wageningen University in the Netherlands and joined Phenomenex in 2020 as Technical Sales Consultant. Koen became part of Phenomenex’s global technical support team in 2022 and assists laboratories in training chromatographers and developing analytical methods.</p></blockquote>

Phenomenex: Recipe for Separation: Crafting Your Perfect HPLC Method

Recipe for Separation: Crafting Your Perfect HPLC Method

Discover how to properly use and maintain your column to maximize its performance and lifespan.

<h4><strong>Key Learning Points</strong></h4><ul><li>Understand how SLE and SPE work and how they are different from each other.</li><li>Evaluate the advantages and limitations of each method for various matrices.</li><li>Select the appropriate technique for analyte chemistry and target sensitivity.</li></ul><h4><strong>Who Should Attend</strong></h4><ul><li>LC or LC‑MS users evaluating or optimizing sample prep workflows.</li><li>Analysts working with challenging biological or environmental matrices.</li><li>Scientists who are unsure when to apply SLE vs. SPE for maximum recovery and cleanliness.</li><li>Method developers seeking to improve robustness, throughput, or reproducibility.</li></ul><blockquote><p><strong>Speaker:</strong> Grace Guo &nbsp;(Senior Technical Specialist, Phenomenex)</p><p>Grace Guo graduated from Johns Hopkins University with a Master’s degree in Material Science and Engineering. She joined Phenomenex Technical Team in 2017 providing technical support globally. With more than 9 years of experience in SPE, HPLC and GC, she delivered 90+ seminars and webinars globally covering food and environmental, clinical, pharma and biopharma industry.</p></blockquote>

Phenomenex: Extraction Face-Off: Choosing Between SLE and SPE

Extraction Face-Off: Choosing Between SLE and SPE

Understand how SLE and SPE work and how they are different from each other.

Choosing Between SLE and SPE

<p><strong>As Sustainable Aviation Fuel (SAF) production expands globally, fuel testing is becoming more demanding. New feedstock pathways and evolving processing routes introduce analytical complexity that can challenge traditional methods.</strong></p><p>Join us for an interactive session on how Combustion Ion Chromatography (C-IC) is transforming fuel testing performance in modern laboratories. We will also provide technical insights into the Thermo Scientific™ Cindion ™ C-IC system architecture and how it supports robust, reproducible results in demanding Oil &amp; Gas applications.</p><h5><strong>In this webinar, you will learn:</strong></h5><ul><li>The analytical challenges contract testing laboratory encounters with emerging fuel matrices for ASTM D7359, and D7566 methods</li><li>How Cindion C-IC System overcomes these challenges</li><li>The measurable improvements achieved in accuracy, compliance, efficiency, and reliability</li></ul><h5><strong>Who should attend?</strong></h5><p>This session is designed for professionals who are:</p><ul><li>Struggling with complex SAF matrices and inconsistent results</li><li>Facing increasing ASTM compliance and regulatory pressure</li><li>Looking to improve reproducibility and reduce rework</li><li>Seeking ways to streamline fuel testing workflows and boost efficiency</li></ul><p>Bring your questions! Jonathan from Saybolt and our C-IC Product Manager, Pavan, will address your technical and application inquiries directly in the live Q&amp;A session.</p><blockquote><p><span><strong>Presenter: </strong></span>Jonathan Chan (Technical Chemist, Saybolt Singapore)</p><p>Jonathan is a Technical Chemist at Saybolt Singapore with experience in petroleum, biofuels, and renewable fuel analysis. His work focuses on developing and validating analytical methods for complex fuel matrices, including trace elemental analysis using advanced techniques such as combustion ion chromatography and ICP-based methods. At Saybolt, Jonathan supports both routine laboratory operations and technical development projects, helping ensure reliable and defensible analytical results for the evolving energy landscape.</p><p><span><strong>Presenter: </strong></span>Pavan Sandhu (Product Manager, Thermo Fisher Scientific)</p><p>Pavan is a Product Manager in the Ion Chromatography &amp; Sample preparation-DIA &nbsp;(IC/SP-DIA) business unit at Thermo Fisher Scientific managing Combustion IC &amp; Discrete Analyzer products.</p><p>Pavan completed her MS in Biomedical Engineering from University of Texas at Dallas and has several years of experience in clinical diagnostics and analytical instrumentation. Prior to joining IC/SP-DIA, she was part of an R&amp;D team at Thermo Fisher’s Clinical Diagnostic Division (CDD) leading product development &amp; design transfer of multiple diagnostic products. In her current role, she is focussed on driving innovative ion chromatography solutions for our customers.<span>.</span></p></blockquote>

Thermo Fisher Scientific: Transforming Fuel Testing Performance with Combustion Ion Chromatography

Transforming Fuel Testing Performance with Combustion Ion Chromatography

Join us for an interactive session on how Combustion Ion Chromatography (C-IC) is transforming fuel testing performance in modern laboratories.

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