Vážení zákazníci,přichází JARNÍ AKCE 2026, během které můžete využít zvýhodněné ceny na široký sortiment spotřebního materiálu pro vaši laboratoř.<h4>Nabízíme vše, co potřebujete pro každodenní provoz i náročné analýzy:</h4><ul><li>kolony pro HPLC, UHPLC, BioLC</li><li>kolony pro GC a IC</li><li>spotřební materiál pro přípravu vzorků</li><li>drobné laboratorní vybavení</li></ul>Kvalitní spotřební materiál je základem spolehlivých výsledků. Využijte proto tuto příležitost a doplňte své zásoby za výhodnějších podmínek.<blockquote>Akce je platná na území ČR od 1. 4. do 31. 5. 2026.Kontaktujte nás jednoduše na e-mailu: chromatografie@pragolab.cz.</blockquote><h4>−30 % HPLC kolony</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products/423">Hypersil GOLD</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/products?category=48&amp;manufacturerItems=3&amp;search=accucore">Accucore</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Syncronis">Syncronis</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Hypercarb">Hypercarb</a></li></ul><h4>−20 % BioLC kolony a kolony pro speciální aplikace</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=MabPac">MabPac</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=ProPac">ProPac</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=PepMap">PepMap</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=EasySpray">EasySpray</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?mainauthorItems=3&amp;search=Acclaim">Acclaim</a></li></ul><h4>−20 % GC kolony</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=TraceGOLD">TraceGOLD</a> — <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=Trace">Trace GC</a> — <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/products?category=7&amp;distributorItems=42&amp;search=TracePLOT">Trace PLOT</a></li></ul><h4>−25 % IC kolony a spotřební materiál</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?instrumentationItems=28-34&amp;mainauthorItems=3&amp;search=columns">Kolony</a> — <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?instrumentationItems=28&amp;mainauthorItems=3&amp;search=EGC">EGC</a> — supresory — vialky</li></ul><h4>−25 % <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/Pragolab_vialka_f386946736/Pragolab_vialka_.pdf">Vialky Pragolab</a></h4><ul><li>Vialky — septa — kity</li></ul><h3>Přehled nejnovější literatury z oblasti spotřebního matriálu od Thermo Scientific v knihovně <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/library?from=2025&amp;instrumentationItems=20&amp;manufacturerItems=2&amp;to=2026">LabRulezLCMS</a></h3><ul><li>Instrument and LC column migration for the purification and analysis of synthetic oligonucleotides (Aplikace | 2025)</li><li>Evolution of Ion-Exchange columns for biologics characterization (Prezentace | 2025)</li><li>Advancing Low flow LC/MS for single cell proteomics with variable flow and 50 cm microfabricated pillar array columns (Prezentace | 2025)</li><li>Wide-ranging polynucleotide separation capabilities using Reversed Phase particles with variable pore geometry (Postery | 2025)</li><li>Evaluation of batch-to-batch consistency of reversed phase HPLC columns for long-term method validation (Postery | 2025)</li><li>Efficient tandem capillary flow LC-MS with short μPAC columns and a single ionization source (Postery | 2025)</li><li>At the intersection between chromatographic performance, ESI efficiency and instrument productivity: nano to capillary flow LC/MS on long μPAC Columns (Postery | 2025)</li><li>Achieving consistent SEC performance through the use of 3 μm, 550 Å monodisperse media in novel bioinert column hardware (Postery | 2025)</li><li>Improved accuracy and identification in label-free quantitative proteomics with the OptiSpray Ion Source and separation cartridge with integrated replaceable emitters (Postery | 2025)</li><li>Accucore columns Resolve all your impurities without feeling the pressure (Ostatní | 2025)</li><li>SureSTART Vial Quick Select Guide (Ostatní | 2025)</li><li>Sensitive cationic lipids impurities analysis with quantitation by charged aerosol detection and simultaneous mass confirmation by MS (Aplikace | 2025)</li><li>Thermo Scientific Viper TQ UHPLC Fittings (Brožury a specifikace | 2025)</li><li>HPLC vials and well plates - Every drop counts (Brožury a specifikace | 2025)</li></ul><h3>Vybrané nejnovější <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinars?companyId=42">webináře</a> Thermo Scientific z oblasti spotřebního materiálu</h3><h5>1. Mastering Single Cell Proteomics: Daily Excellence with μPAC Neo Plus Trap-and-Elute Workflow</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5789">ZÁZNAM</a> | Proběhlo Út, 27.1.2026</li></ul>Zjistěte, jak dosáhnout robustní, vysoce citlivé proteomiky jednotlivých buněk pomocí systému μPAC Neo Plus trap-and-elute LC-MS se systémy Vanquish Neo a Orbitrap.<h5>2. Monodisperse inert HPLC columns for large molecule analysis in biopharma</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5729">ZÁZNAM</a> | Proběhlo Út, 9.12.2025</li></ul>Připojte se k nám na zajímavém webináři o pokročilých chromatografických metodách pro analýzu velkých biomolekul.<h5>3. Reduce analyte loss in LC workflows: The role of vial and surface chemistry</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/webinar/5675">ZÁZNAM</a> | Proběhlo Po, 17.11.2025</li></ul>Objevte strategie pro zvýšení výtěžnosti vzorků proteinů a peptidů. Zjistěte, jak materiály vialek a nespecifická vazba ovlivňují výsledky LC, a získejte tipy pro zlepšení reprodukovatelnosti testů.

Pragolab JARNÍ SLEVY 2026

<h4>Využijte jedinečné příležitosti a vyzkoušejte si vybrané GC / LC kolony a další spotřební materiál ZDARMA!</h4><blockquote><ul><li>promo kód: 2555</li><li>nabídka platná do: 7. 8. 2026</li></ul></blockquote><h5>Na co se akce vztahuje:</h5><ul><li>kompletní portfolio GC kolon – včetně nejnovějších <a target="_blank" rel="noopener noreferrer" href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/br_5q_gc_column_campaign_5994_7596en_agilent_baca38e505.pdf">5Q GCMS</a></li><li>většinu HPLC kolon - včetně nejnovějších ultra-inertních kolon <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/br_altura_hplc_columns_ultra_inert_technology_5994_8687en_agilent_517a6c36a7.pdf">Altura</a></li><li>vybraný spotřební materiál pro přípravu vzorků - včetně nejnovějších <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/article/5711">SPE kolonek PFAS WAX</a> s duální fází</li><li>a některé produkty Crosslab a OneNote – včetně <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/flyer_quick_connect_fittings_infinitylab_5991_5164_EN_agilent_0d67eecd72/flyer-quick-connect-fittings-infinitylab-5991-5164EN_agilent.pdf">Quick Connect Agilent InfinityLab Quick Connect Fittings</a> a <a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/fl_infinitylab_quickturn_5994_7793en_agilent_819d965d19.pdf">Quick Turn fitinků</a> Agilent InfinityLab Quick Turn Fittings.</li></ul><blockquote><a target="_blank" rel="noopener noreferrer" href="https://www.hpst.cz/sites/default/files/download/2025-12/2555EligibleProductsList_25Sep25-komprimovan%C3%BD.pdf">Kompletní nabídka produktů v akci "Try and Buy"</a></blockquote>Lhůta 50 dnů na vyzkoušení bez platby předem. V případě nespokojenosti vrácení produktu zpět

Altium Akce Try and Buy na GC, HPLC kolony a jiný spotřební materiál

Tradiční postupy purifikace proteinů se v mnoha laboratořích stále opírají o ultracentrifugaci a dlouhé inkubační kroky. Tyto přístupy často zahrnují vícekrokové protokoly, prodloužené doby analýzy a použití specializovaného vybavení. V důsledku toho mohou být pracovní postupy složité, časově náročné a náchylné k variabilitě výsledků.Purifikace proteinů pomocí magnetických kuliček nabízí rychlejší, jednodušší a efektivnější alternativu, protože umožňuje selektivní zachycení a rychlou magnetickou separaci.<h3>Omezení konvenčních metod purifikace proteinů</h3>Ve standardních laboratorních postupech purifikace proteinů obvykle zahrnuje:<ul><li>Více pracovních kroků</li><li>Centrifugaci a ekvilibraci kolony</li><li>Závislost na specializovaném vybavení</li><li>Dlouhé doby zpracování</li></ul>Tyto faktory přinášejí několik výzev:<ul><li>Zvýšená složitost pracovních postupů</li><li>Variabilita mezi jednotlivými cykly, což snižuje reprodukovatelnost</li><li>Riziko ztráty vzorku při manipulaci, zejména u malých objemů vzorků nebo omezených množství cílových látek</li><li>Omezená kapacita kvůli manuální manipulaci</li></ul><h3>Princip purifikace pomocí magnetických kuliček</h3>Magnetické kuličky umožňují selektivní zachycení proteinů díky funkcionalizaci povrchu.Každá kulička je potažena streptavidinem, na který se váže biotinylovaný ligand. Tento ligand lze vybrat na základě cílového analytu a může zahrnovat:<ul><li>Protilátky</li><li>Aptamery</li><li>Fragmenty antigenu</li></ul>Tento krok poskytuje kuličkám specificitu potřebnou pro vazbu cílového analytu.<h3>Materiály a klíčové komponenty</h3><h4>Magnetické kuličky</h4><ul><li>Povrch potažený streptavidinem</li><li>Funkcionalizace biotinylovanými ligandy</li></ul><h4>Ligandy</h4><ul><li>Voleny dle cílového analytu</li><li>Navázané na kuličky před purifikací</li></ul><h3>Postup krok za krokem</h3><h4>1. Aktivace kuliček</h4>Navázání biotinylovaného ligandu na kuličky.<h4>2. Promývání</h4>Odstranění nečistot po funkcionalizaci.<h4>3. Inkubace se vzorkem</h4>Navázání cílového analytu na ligand.<h4>4. Magnetická separace</h4>Umístění vzorku na magnet:<ul><li>Magnetické kuličky (s navázaným analytem) se přitáhnou ke stěně nádoby</li><li>Kapalná fáze se oddělí</li></ul><h4>5. Promývání</h4>Promytí kuliček, zatímco zůstávají imobilizovány magnetickým polem, pro odstranění:<ul><li>Slabých interakcí</li><li>Nespecifických vazeb</li></ul><h4>6. Eluce</h4>Uvolnění analytu pomocí elučního roztoku.<h3>Následné aplikace</h3>Po eluci je purifikovaný vzorek připraven k další analýze, včetně:<ul><li>LC-MS</li><li>Western blot</li><li>ELISA</li><li>Testování aktivity</li></ul><h3>Výhody pracovních postupů založených na magnetických kuličkách</h3>Purifikace založená na magnetických kuličkách nabízí několik klíčových výhod:<h4>Rychlost a jednoduchost</h4><ul><li>Není nutná centrifugace</li><li>Není potřeba složité vybavení</li></ul><h4>Zlepšená reprodukovatelnost</h4><ul><li>Konzistentní magnetická separace</li><li>Snížená variabilita mezi jednotlivými cykly</li></ul><h4>Snížená ztráta vzorku</h4><ul><li>Méně kroků při manipulaci</li><li>Vhodné pro malé objemy vzorků nebo omezená množství cílových látek</li></ul><h4>Škálovatelnost a kapacita</h4><ul><li>Možnost automatizace</li><li>Podporuje formáty mikrodestiček:<ul><li>96-jamkové destičky</li><li>384-jamkové destičky</li></ul></li></ul><h3>Vlastnosti magnetických kuliček Biozen™</h3>Magnetické kuličky Biozen™ od společnosti Phenomenex zahrnují několik konstrukčních vlastností:<ul><li>Hydrofilní kopolymerový povrch<ul><li>Snižuje nespecifické hydrofobní interakce</li></ul></li><li>Biotinylovaný BSA spacer<ul><li>Působí jako spojovací článek ke streptavidinu</li><li>Pomáhá orientovat záchytné ligandy</li></ul></li><li>Monodisperzní ligandy<ul><li>Optimalizovaná vazebná kapacita</li></ul></li></ul>Tyto vlastnosti přispívají k:<ul><li>Snížení nespecifické vazby</li><li>Lepší reprodukovatelnosti</li><li>Čistším eluátům</li></ul><h3>Kdy použít magnetické kuličky</h3>Čištění pomocí magnetických kuliček je zvláště vhodné, když:<ul><li>Objemy vzorků jsou malé</li><li>Je vyžadována rychlá doba zpracování</li><li>Je omezené vybavení</li><li>Jsou potřebné pracovní postupy s vysokou propustností</li></ul><h3>Závěr</h3>Purifikace proteinů pomocí magnetických kuliček zjednodušuje tradiční pracovní postupy tím, že eliminuje odstřeďování a snižuje složitost. Díky selektivnímu zachycování a účinné magnetické separaci umožňuje rychlejší zpracování, lepší reprodukovatelnost a kompatibilitu s automatizovanými systémy s vysokou propustností.

Purifikace proteinů pomocí magnetických kuliček: Rychlejší a efektivnější pracovní postup

Akademie

Video

Článek

Produkt

Je autorizovaným distributorem společnosti Agilent Technologies pro Českou republiku v oblastech chromatografie (GC, HPLC, UHPLC, kapilární elektroforéza) a hmotnostní spektrometrie (GC/MS, LC/MS, CE/MS), disolučních aparátů, molekulární (UV-Vis, UV-Vis-NIR, fluorescence, FTIR analyzátory a mikroskopy) a atomové spektroskopie (ICP-OES, ICP-MS, AAS), a v neposlední řadě také v oblasti molekulární biologie a genomiky (analýza nukleových kyselin a proteinů, microarray skenery a skla, PCR & RT-PCR, qPCR, řešení pro NGS, reagencie pro mutagenezi a klonování, sondy FISH a další).

Altium International

Společnost Phenomenex je celosvětovým technologickým lídrem, který se zabývá vývojem nových řešení v oblasti analytické chemie, jež řeší požadavky analytiků na separace a purifikace v průmyslových, vládních a akademických laboratořích.

Phenomenex

Jsme jedním z lídrů na českém a slovenském trhu v oblasti laboratorních zařízení a svým zákazníkům přinášíme špičkové přístroje z oboru analytické chemie a fyzikálních měření více než 25 let.

Pragolab

Agilent Technologies

Bruker

Thermo Fisher Scientific

C&EN

Shimadzu Corporation

SCIEX

Evosep Biosystems

Waters Corporation

The upcoming webinar will delve into the latest advancements in pneumococcal polysaccharide vaccines and pneumococcal conjugate vaccines (PCVs), focusing on their development, production, and quality control. Key topics include the introduction of a new USP general chapter for the SEC-MALS method, the challenges of serotype replacement, and the evolution of PCVs to include more serotypes.The session will explore the critical role of SEC-MALS in ensuring vaccine consistency and stability, highlighting its advantages over traditional analytical techniques, as well as the benefits of FFF-MALS for ultra-high molecular weight PCV analysis.Key Learning Objectives:<ul><li>Understanding Pneumococcal Vaccines: Gain insights into the differences between pneumococcal polysaccharide vaccines and PCVs, their historical development, and their impact on public health.</li><li>Challenges in Vaccine Development: Learn about serotype replacement and the on-going efforts to develop vaccines that cover a broader range of serotypes.</li><li>Analytical Techniques: Explore the advantages of using SEC-MALS for multi-attribute quantification of vaccines, including its role in process development and quality control.</li><li>Future Directions: Discover the latest advancements in PCV development, including the push towards 30+ valent vaccines and the role of emerging technologies in vaccine production.</li></ul>Who Should Attend:<ul><li>Vaccine Researchers: Researchers involved in vaccine development will benefit from understanding the latest advancements in pneumococcal vaccines and the analytical techniques used to ensure their efficacy and safety.</li><li>Quality Control Specialists: Professionals responsible for the quality control of vaccines will gain valuable insights into the use of SEC-MALS for monitoring vaccine consistency and stability.</li><li>Pharmaceutical Manufacturers: Individuals working in the production and development of vaccines will find the discussion on new analytical methods and the challenges of serotype replacement crucial for improving vaccine formulations.</li></ul><blockquote>Presenter: Katayoun Morakabi, Ph.D. (Application Scientist)Katayoun Morakabi (Kat) is an application scientist on the Analytical Sciences team at Wyatt Technology, a portfolio of Waters corporation. Kat assists customers in identifying the best light scattering solutions for their analytical needs by performing feasibility studies and leveraging her expertise in multi-angle light scattering. She earned her Ph.D. in Biochemistry from the University of Texas at Dallas, where her doctoral research focused on characterizing the structure, dynamics, and functional outcomes of proteins. If you attend Light Scattering University at Wyatt's headquarters in Santa Barbara, you might meet Kat in person as one of your instructors.</blockquote>

Waters Corporation: Advances in Polyconjugate Vaccine Development: Leveraging SEC-MALS for Enhanced Quality Control and Efficacy

Advances in Polyconjugate Vaccine Development: Leveraging SEC-MALS for Enhanced Quality Control and Efficacy

This webinar explores advances in pneumococcal vaccines, focusing on development, production, and quality control.

Polyconjugate Vaccines: SEC-MALS for Quality & Efficacy

Drug discovery is the process through which potential new medicines are identified. It involves a wide range of scientific disciplines, including biology, chemistry and pharmacology.The complexity in drug development has increased manifolds over the past 40 years, requiring preclinical testing, investigational new drug applications, and completed clinical testing before marketing approval from the FDA.Generally, new drug applications or biologics license applications are reviewed comprehensively before approval, and then drug performance is resubmitted to regulatory agencies for post-marketing studies. The overarching goal is to bring more efficient and safer treatments to the patients as quickly as possible after a thorough medical evaluation.In this webinar we will look into how the Evosep One with high-throughput and sensitivity is a good fit when developing new drug applications or biologics license applications.&nbsp;<blockquote>Presenter: TBA soon</blockquote>

Evosep Biosystems: Accelerating Drug Discovery with high-throughput proteomics

Accelerating Drug Discovery with high-throughput proteomics

In webinar we will look into how Evosep One with high-throughput and sensitivity is good for developing drug applications or biologics license applications

<h3>Boost your method development capabilities</h3>Creating a reliable and successful method can be challenging at times, especially if you’re dealing with complex matrices. Finding the right balance can take time. Samples need to be properly prepared and figuring out the right parameters for speed and sensitivity involves trial-and-error, which can be time-consuming.We want to help make method development a whole lot easier for you. In our Build an LC-MS/MS method with me webinar series, we will guide you through the steps to create a successful method. These 45-minute sessions are led by an application chemist who will focus on building a method that can be used for detecting illicit drugs in seafood using real data collected in our lab.<h4>Part 3: Constructing confidence in your mass spectrometry method</h4>In this session you will:<ul><li>Understand commonly used terms for validation or verification and how to establish lower limits of quantitation of the method versus the instrument</li><li>Differentiate precision versus accuracy and how to test for either</li><li>Get final tips on method validation</li></ul><blockquote>Presenter: Diana Tran (Applications Manager, SCIEX)</blockquote>

SCIEX: Build an LC-MS/MS method with me: A 3-part educational webinar series

Constructing confidence in your mass spectrometry method

Understand commonly used terms for validation or verification and how to establish lower limits of quantitation of the method versus the instrument

 Constructing confidence in your mass spectrometry method

The key in translational research is to address the right question for each specific disease. In this talk, Dr. Chiara Guerrera will present case studies of three rare genetic diseases, highlighting how we selected the most appropriate proteomics analyses to gain deeper insights into each condition. Their analysis cover plasma, urine, and skin biopsies, as well as phosphorylation events in tissue. Dr. Chiara Guerrera will focus on the optimization of each analytical method and share our insights and lessons learned. Why you should join the webinar:<ul><li>Learn more about the benefit of proteomics approaches for rare genetic disease studies</li><li>Witness how plasma, urine, skin and phosphoproteomics approches are applied for translational research</li><li>Get an insight of the full timsTOF proteomics ecosystem benefits for translational research</li></ul><blockquote>Presenter: Chiara Guerrera, Ph.D., IRHC, HDR (Head of Necker Proteomics, Faculty of Medicine, University Paris Cité, France)</blockquote>

Bruker: Tailored Proteomics for Biomarker Research in Genetic Diseases: From Biofluids to Tissue Biopsies

Tailored Proteomics for Biomarker Research in Genetic Diseases: From Biofluids to Tissue Biopsies

Case studies of three rare genetic diseases, highlighting how we selected the most appropriate proteomics analyses to gain deeper insights into each condition

Tailored Proteomics for Biomarker Research in Genetics

Identifying drug metabolites is crucial for understanding drug behavior, efficacy, and safety, but it poses significant challenges due to the complexity and variability of biotransformation products.&nbsp;In this webinar, we describe a novel hi-res LC/MS strategy for drug metabolite identification and demonstrate with several known drugs. This approach combines cutting-edge software to predict drug biotransformations, search MS/MS data against these structures using molecular fingerprinting, and map identifications to molecular features from differential analysis of drug-treated liver microsomes. The workflow addresses the challenge of identifying drug biotransformation products that are often absent from known spectral libraries.Key Learning Objectives:<ul><li>Learn about the challenges in detecting drug biotransformation products, particularly those absent from known spectral libraries, and understand the need for innovative solutions.</li><li>Explore a new high-resolution LC/MS approach that combines software to predict drug biotransformations, search MS/MS data using molecular fingerprinting, and map identifications to molecular features from chemometrics software.</li><li>See practical demonstrations of the workflow applied to characterize several known drugs and their metabolites, enhancing your ability to implement similar strategies for novel drugs in your own research.</li></ul>Who Should Attend:<ul><li>Laboratory managers</li><li>Chromatographers</li><li>New product developers</li></ul><blockquote>Presenter: Mark Sartain, PhD (Application Development Scientist, Agilent Technologies)</blockquote>

C&EN: Empowering Drug Metabolite Identification with a Novel LC/MS Software Workflow

Empowering Drug Metabolite Identification with a Novel LC/MS Software Workflow

In this webinar, we describe a novel hi-res LC/MS strategy for drug metabolite identification and demonstrate with several known drugs. 

LC/MS Drug Metabolite Identification

Column selection for SEC/GPC is a key challenge in biomolecular seperations. Understanding when to choose what type of column is critical for developing successful SEC/GPC methods.In this webinar, we will highlight the main factors that define a strong SEC column and illustrate how they benefit the analysis of different modalities, including AAVs, VLPs, and large oligonucleotides.<h5>Key learning objectives</h5><ul><li>Learn how to choose the right SEC/GPC column for biomolecular analysis.</li><li>Discover key factors: particle size, pore volume, detector compatibility, and more.</li><li>Explore workflow examples for better understanding.</li></ul><h5>Who should attend?</h5><ul><li>Analytical chemists focusing on SEC/GPC for biomolecules, including AAVs, VLPs, and large oligonucleotides.</li></ul><blockquote>Presenter: Katrien Sabbe, PhD (Biopharma Business Development Manager Chemistries and Supplies, Agilent Technologies)Katrien Sabbe holds a PhD in organic synthesis (Ghent University, Belgium). For more than 25 years she is working in the field of Chromatography Columns and Supplies, first at VWR International and later at Varian Analytical Instruments, which was acquired in 2011 by Agilent Technologies. Currently she is the EMEA Biopharma Business Development Manager at Agilent Technologies Chemistries and Supplies Division.</blockquote>

Agilent Technologies: Advanced Column Selection for SEC/GPC Analysis of Biomolecules

Advanced Column Selection for SEC/GPC Analysis of Biomolecules

In this webinar, we will highlight the main factors that define a strong SEC column

Column Selection for SEC/GPC Analysis of Biomolecules

Preparative chromatography has been an integral component of pharmaceutical discovery and development for the past fifty plus years. The past thirty-five years have seen great advances in small molecule purification. Purification techniques have advanced from those centered around large irregular particles operating under low pressure to more efficient high-performance technologies utilizing small spherical particles. Technologies now routinely used include preparative HPLC, SMB, and SFC. At the discovery scale, these advances allow routine and rapid chiral and achiral purification of the large numbers of molecules synthesized at this stage of pharmaceutical R&amp;D. For pharmaceutical development these advances allow chromatographic purifications using HPLC and SMB to be a standard tool in the process development toolbox. The large number of pharmaceutical chiral SMB operations at the manufacturing scale has shown that chromatography can be a cost-effective alternative to asymmetric synthesis or other resolution techniques.This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company. Multiple real-life examples from ug to metric ton scale supporting Discovery through Manufacturing activities will be presented. In addition, the importance of personnel, facility, automation, and information systems for a successfully integrated purification platform will be discussed.<blockquote>Presenter: Larry Miller (Associate Director, Novartis)Larry Miller is an Associate Director in the Global Discovery Chemistry Group at Novartis in Cambridge, MA responsible for analytical and purification support for Discovery. He has spent his career performing small molecule achiral and chiral purifications at the mg to multi-kg scale using a variety of chromatographic techniques. Larry has over 40 peer-reviewed publications and over 45 presentations at scientific meetings. He also co-authored “Modern Supercritical Fluid Chromatography: Carbon Dioxide Containing Mobile Phases” with David Pinkston and Larry Taylor. Larry also serves as co-instructor for an SFC short course which has been held at numerous chromatography conferences in the United States, Europe, and Asia over the past fifteen years. In addition, Larry is the current president of the Green Chemistry Group, a not-for-profit group that organizes the annual SFC conference.</blockquote>

Agilent Technologies: Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

Small Molecule Chromatographic Purification in Pharmaceutical R&D and Manufacturing

This presentation will discuss the critical role small molecule purification plays within a pharmaceutical company.

Small Molecule Purification in Pharma R&D and Manufacturing

<div><div>High-resolution mass spectrometry (HRMS) has become the technique of choice for screening applications in forensic and doping laboratories. The Forensic Toxicology Screening Application Solution from Waters was first released in 2015 and has continued to evolve over the years; the current system comprises an ACQUITY UPLC I-Class and Xevo G3 QTof Mass Spectrometer together with a dedicated application package for forensic toxicology and brings the power of high resolution and accurate mass data to the routine laboratory.The current solution is powered by an application-based software package, waters_connect which provides a single platform for LC and LC-MS applications that accelerates end-to-end scientific workflows, realizing new standards of usability, scientific outcome and compliance.In addition to the current solution with the Xevo G3 QTof, Waters have recently launched the Xevo MRT, an ultra-high resolution mass spectrometer, achieving mass resolution of up to 100K and mass accuracies typically less than 500 parts-per-billion. With the introduction of this new technology into the forensic and toxicology arena, it has the capabilities to change the way we view mass accuracy for targeted analysis, providing even more assurance when reporting results, but also the extra confidence when performing discovery type analysis, in search of novel psychoactive substances.<h4>Key learning objectives:</h4><ul><li>Learn about the development and advancement of the Waters Forensic Screening Application Solution.</li><li>Recognize the benefits of using a single platform for improved usability, scientific outcomes and compliance.</li><li>Assess the significance of Xevo MRT’s ultra-high resolution and mass accuracy in forensic and toxicology applications.</li></ul>For Forensic Toxicology Use only.<blockquote>Presenter: Nayan Mistry (Senior Clinical Application Scientist, Forensic Toxicology, Research &amp; Development, Waters Corporation)Nayan Mistry is a Senior Application Scientist at Waters Corporation. Nayan joined Waters in 2009 and is part of a global team dedicated to performing research and development of applications and analytical solutions for Forensic Toxicology.Nayan’s key focus is on toxicology screening and he has expertise with both nominal mass and accurate mass-based screening using UNIFI and Waters_Connect application software. Nayan plays a key role in the development of these solutions and in the expansion of associated libraries. Currently Nayan is playing a key role in the development and support of the recently launched Xevo G3 QTof mass spectrometer in addition to other responsibilities for the nominal mass applications. His favourite aspect of the role is customer support - Nayan is greatly involved in global on-site customer support and training and has presented his work at conferences worldwide.</blockquote></div></div>

Waters Corporation: Revolutionizing High Resolution Mass Spectrometry and Forensic Toxicology: Key Updates and Solutions

Revolutionizing High Resolution Mass Spectrometry and Forensic Toxicology: Key Updates and Solutions

Learn about the development and advancement of the Waters Forensic Screening Application Solution

Revolutionizing HRMS and Forensic Toxicology

<div><div id="inner_content_body_text" data-content-id="394785">Single-cell analysis is a new and rapidly growing field that is shedding new insight into the cellular mechanisms behind the pathogenesis and treatment of infectious diseases and cancer. Cells, even within a single tissue, culture or biological fluid are highly heterogenous. Analysis of bulk material therefore only provides an average value, meaning that information is lost. Single cell transcriptomics and proteomics are already making major advances to understand infection, immunity and drug delivery. Single cell lipidomics and metabolomics are highly desirable to understand the endpoint of metabolism, but currently lag behind the other “omics” at single cell level. Similarly, sub-cellular analysis is highly desirable to understand cellular rearrangement in response to stressors such as pathogens and radiation. The SEISMIC facility for spatially resolved single and sub-cellular “omics” is a new multi-user facility, that aims to advance single cell and sub-cellular measurements, together with and for the benefit of bioscientists across the UK. At SESIMC, we use capillary sampling to sample live cells or sub-cellular compartments under microscope observation. This method of sample capture can be coupled to chromatographic separation, which offers additional advantages in mitigating matrix effects and aiding peak assignment. We will show how live single-cell lipidomics can be achieved using capillary-sampled cells as well as microfluidics. We will show the expected performance from state-of-the-art mass spectrometry systems. We will also show how these technologies can be applied to reveal how single cell lipid profiles of cancer cells respond to drug and radiation treatment. We will further report on progress towards sub-cellular sampling and mass spectrometry metallomics and proteomics analysisLearning Objectives:<ul><li>How does the SEISMIC facility enable spatially resolved single-cell and sub-cellular 'omics' analysis?</li><li>How can single-cell lipidomics be applied to study cellular responses to drug and radiation treatments in cancer cells?</li></ul> Webinars will be available for unlimited on-demand viewing after live event.<blockquote>Presenter: Melanie Bailey, PhD (Professor, University of Surrey)Melanie Bailey is Professor of Analytical Science at the University of Surrey. She is Director of the Wolfson Centre of Excellence for Bioanalytical Science, which brings together expertise in “omics”, biomarker discovery and imaging. This includes SEISMIC, a new BBSRC national facility for spatially resolved single and sub-cellular “omics” , new instrumentation for multimodal imaging at the UK National Ion Beam Centre and bulk metabolomics, proteomics and metallomics. She is a member of the international advisory board for The Ion Beam Analysis conference series, International Nuclear Microprobe conference series and the Bragg Institute. She is a Trustee of the Analytical Chemistry Trust Fund and ran the London Marathon to raise money for Analytical Science in 2024. She will commence a term as Editor in Chief of the Royal Society of Chemistry’s longest running journal “Analyst” in September 2024.</blockquote></div></div>

Thermo Scientific: Towards sub-cellular mass spectrometry omics: The SEISMIC facility for spatially resolved single and sub-cellular omics

Towards sub-cellular mass spectrometry omics: The SEISMIC facility for spatially resolved single and sub-cellular omics

In this webinar you will learn how does the SEISMIC facility enable spatially resolved single-cell and sub-cellular 'omics' analysis.

SEISMIC facility - spatially resolved single cellular omics

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. &nbsp;We will examine the break-through use of SFC with LabSolutions MD AQbD method development software to model and optimize peptide separations. &nbsp;The topic of Exposed Polar Surface Area analysis by SFC will be discussed and how its application to cyclic peptide therapeutics speeds up drug candidate selection. &nbsp;We will share recent developments in SFC separations for oligonucleotides, along with other enabling solutions for micro-prep SFC (analytical scale fractionation), hybrid UHPLC-SFC systems, and more.We will host a Q&amp;A session after the presentation to address any questions or comments.<blockquote>Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.</blockquote>

Shimadzu: Innovations in SFC Applications and Technology for Pharma and Biopharma

Innovations in SFC Applications and Technology for Pharma and Biopharma

Join us for this event that will discuss how Shimadzu SFC technology is being used to drive innovation throughout the drug development process. 

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