Helpful ‘hints’ for Protein SEC Success
Are you currently using or planning to use SEC for characterizing your protein or mAb sample or is SEC a separation technique that is new to you?
Topics addressed will include:
- Why use SEC
- Importance of Pore size and Particle size selection
- How To select and tips for using your column
- Mobile phases and buffer considerations
- Using detergents
- Common detectors
- Troubleshooting
Taking time up front to consider the important parameters for your SEC separation will help ensure a method that is reliable and robust.
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
