Enhancing LC-MS analysis of oligos, peptides, and glycans using hybrid surface technology

Development of biopharmaceutical drug products such as oligonucleotides, peptides, and glycans requires robust and sensitive analytical methods to ensure safe and effective products. Conventional LC systems that employ metal columns and flow paths can interact with analytes through metal-ion mediated adsorption and cause poor peak shape, tailing, and diminished recovery. Common strategies to suppress these interactions include hardware passivation or column conditioning, which may create different challenges and drawbacks for biopharmaceutical analysis.

In this webinar, a team of scientists from Waters Corporation (Jennifer Nguyen, Jacob Kellett, and Dr. Xiaoxiao Liu) will describe the benefits of the ACQUITY PREMIER Solution with MaxPeak High Performance Surface Technology in mitigating problematic metal interactions. Learn how improved recovery, peak shape, and reproducibility of sensitive analytes can be achieved without the need for system or column conditioning, while combination with a binary pump configuration enables highly reproducible gradients at low flow rates to be achieved with exceptional precision.

Key learning objectives:

• Learn how the performance, sensitivity, and reproducibility of oligo, peptide, and glycan analyses can be negatively affected by metal interactions
• Explore the advantage of a new hybrid surface technology for LC hardware that can mitigate these metal interactions and improve reproducibility, recovery, and peak shape
• Understand the necessity of a pump configuration that can deliver accurate and reproducible gradients at low flow rates

Who should attend?

• Lab directors, managers, and analytical scientists who are involved in biopharmaceutical or antisense therapy development, manufacturing, and/or QC
• Lab managers or scientists that require better reproducibility for gradient separations at low flow
• Bioanalytical scientists performing quantitative studies and seeking to improve detection limits or sensitivity

Presenter: Jennifer Nguyen (Principal Scientist, Waters Corporation)

Jennifer Nguyen is a principal scientist within Chemistry R&D at Waters Corporation. Since joining Waters in 2015, she has worked on the evaluation and application of new column technologies and consumables for both large and small molecules, including the CORTECS solid-core family, TORUS SFC columns, and the BioResolve product line. Her current research centers on improving LC-MS and sample preparation methods for reversed-phase characterization and quantitation of large molecules (intact, subunit, peptides) and for oligonucleotides. She was previously employed as a research and development engineer at Thermo Fisher Scientific where she developed new column consumables, applications, packing, and quality control processes with a focus on solid-core, sub 2 µm particles, and nanoflow technology.

Presenter: Jacob Kellett (Associate Scientist, Waters Corporation)

Jacob Kellett is an associate scientist in Waters’ Scientific Operations group and has been with Waters for two years. His work has primarily been in applications, hardware analysis, and developing experiments to evaluate Waters’ novel technology, with a focus on the evaluation of new methods and technology against legacy techniques to determine method compatibility and the improvements offered by these new techniques.

Presenter: Dr. Xiaoxiao Liu (Senior Scientist, Waters Corporation)

Xiaoxiao Liu is a senior scientist within Chemistry R&D at Waters. In her past three years at Waters, she has been involved in new product development activities, specializing in the fundamentals of released glycan analysis, affinity-based sample preparations and the optimization of peptide mapping techniques. Prior to joining Waters, Xiaoxiao interned with Genentech to provide analytical support for minor variants characterization of therapeutic proteins and completed her Ph.D. studies at Michigan State University where she applied MS and NMR-based characterization strategies to study plant metabolites.

Moderator: Sarah Thomas (Editorial Team, SelectScience)

Sarah studied biology at the University of Bath, UK, and has worked in the Pharmaceutical Sciences Department of St. Jude Children’s Research Hospital, Memphis. As a member of the Editorial team, Sarah plays an integral role in shaping the content on SelectScience.