Use of the Andrew+ Robot and OneLab Automated Liquid Handling Platform for Bacterial Endotoxin Testing
A bacterial endotoxin test (BET) is a requirement for any injectable drug or medical device to ensure safety. This can be accomplished by performing a Limulus Amebocyte Lysate (LAL) based in vitro endotoxin detection assay such as Pyrochrome®.
This 96 well microplate based kinetic chromogenic assay is accomplished through numerous pipetting steps required for the preparation of the standard curve positive and product controls (PPCs). Placement of the samples and standards, and reagent dispensing in the 96 well plates are tedious steps in the process.
The complexity of these assays would benefit from the simplicity and labor saving benefits of accessible liquid handling methods. We will present an overview of the Pyrochrome® assay configuration, the method, and results generated with Andrew+ and OneLab.
In this webinar you’ll learn:
- How the Andrew+ and OneLab can be configured to execute the Pyrochrome® kinetic chromogenic assay
- Results of evaluating Andrew+ Pipetting Robot and OneLab automation of the Pyrochrome® kinetic chromogenic assay
- Benefits of automating LAL based endotoxin testing methods
Who should attend:
- Laboratory Managers
- Quality Control Managers
- Assay Development Scientists
- Quality Control Analysts
Presenter: Robert D’Ordine Ph.D. (Senior Research Scientist, Associates of Cape Cod, Inc. (ACC))
Robert is a Senior Research Scientist in Research and Development at Associates of Cape Cod, Inc. Robert is an author on twenty peer reviewed publications and patents and has over twenty years of experience in biotechnology working in areas ranging from genomics and protein characterization to biochemistry and diagnostics. Robert has ten years of experience with LAL based assays and specializes in glucan detection methods.
In his current role, Robert interfaces with quality control, product development, clinical development and technical services engaging in automated assay adaptation, methods development, design and analysis of experiments, and technical problem solving.
Presenter: Veronika Wills (Technical Services Manager, Associates of Cape Cod, Inc. (ACC))
Veronika has over 13 years of experience in endotoxin testing, and currently manages the global technical team at ACC and is based at ACC’s US Headquarters in East Falmouth, Massachusetts.
Veronika is a subject matter expert when it comes to endotoxin testing and often provides expert sessions at global events focused on BET products and processes. Most recently Veronika has been speaking on the topic of recombinant technology as it relates to BET in the industry and abroad.
Veronika is a key contributor to ACC’s sustainability initiatives and spokesperson on ACCs related projects, products and services.
Moderator: Mike Auerbach (Editor-in-Chief, American Pharmaceutical Review)
