Emerging LC-MS Techniques to Characterize AAV at the Subunit and Intact Levels
To characterize the constituent capsid proteins, hydrophilic interaction chromatography, operated under denaturing conditions, can be coupled with mass spectrometry (HILIC-MS). This method was successfully applied to characterize a wide variety of AAV serotypes to achieve unambiguous serotype identification, PTM characterization, and stoichiometry assessment. In addition, they will also discuss the development and application of a rapid online native MS method to assess and potentially quantify empty, partially- and fully-filled capsids.
Key learning objectives:
- Learn about ways to analyze adeno-associated capsids with different MS methods
- See methods developed to achieve serotype identification, PTM characterization, and stoichiometry assessment
- Rapid online analytical strategies to quantify ratios of empty, partially- and fully-filled capsids
Who should attend:
- Biopharmaceutical Research Scientists
- Analytical Scientists from Biopharma companies working on or interested in gene therapy
- PhD students in any field of biotherapeutic drug development
- Scientists looking to advance knowledge of gene therapy
Presenter: Anita Liu (Lead R&D Specialist, Regeneron)
Anita Liu is a Lead R&D Specialist in the Analytical Chemistry group at Regeneron Pharmaceuticals in Tarrytown, NY. She received her B.S.E degree in Chemical Engineering from Princeton University and a M. Eng degree in Chemical Engineering from Cornell University. Before joining Regeneron in 2013, Anita previously worked at ExSAR, where she focused on using higher order structure analysis for antibody drugs by hydrogen-deuterium exchange MS. In her current role, she supports the in-depth characterization of therapeutic proteins for drug development and regulatory filings. She also has a research focus of developing intact MS-based methods for improved protein drug characterization.
Presenter: Victoria Cotham (Analytical Scientist, Regeneron)
Victoria Cotham is currently a Scientist in the Analytical Chemistry Group at Regeneron Pharmaceuticals, where she focuses on developing native intact mass methods for the characterization of biotherapeutic protein modalities across various stages of drug development. She earned her Ph.D. in Analytical Chemistry from the University of Texas at Austin, where she developed methods that combined ultraviolet photodissociation (UVPD) with top-down and middle-down mass spectral approaches for the characterization of mAbs and polyclonal antibody mixtures. Prior to joining the team at Regeneron in 2018, she worked as a Staff Scientist in the Proteomics Laboratory at NYU Langone Health, where she performed MS-based epitope mapping and large scale quantitative proteomic workflows.
