Biopharma Symposium 2021 Day 2

Summary

Automated MHC-Associated Peptide Enrichment for Immunopeptidomics Analysis Using Agilent AssayMAP Bravo Large Capacity Cartridges

Immunopeptidomics is generally considered more challenging in part due to the fact that most MHC-associated peptides are extremely low in abundance compared to other cellular peptides. One way to address this challenge is to immunoprecipitate the MHC-I complexes from large amounts of cell lysate using an anti-MHC-I antibody coupled to a solid phase. The MHC-associated peptides are then isolated from the complex and identified by mass spectrometry. This workflow uses the AssayMAP Bravo platform for automated immunoaffinity purification and peptide cleanup. In this study, an Infinity UHPLC Nanodapter converted standard LC flow to nanoflow. The nanoflow LC system was then coupled with a 6550 iFunnel Q-TOF LC/MS using data-dependent acquisition (DDA) for peptide analysis. The combination of high-quality sample preparation and a sensitive LC/MS system provide a reproducible and scalable solution to meet the challenges of studying MHC-I peptidomics.

Presenter: Shuai Wu (Senior Application Scientist, Agilent Technologies, Inc.)

Shuai graduated from University of California, Davis with a PhD in Analytical Chemistry in 2012. With in-depth knowledge of Mass Spectrometry, he joined Agilent in 2016 as a senior application scientist focusing on biopharma and proteomics application development using AssayMAP Bravo. In this role, Shuai has been working actively to generate workflows using AssayMAP Bravo. He delivers high quality workflow and product trainings to field, and presents at different conferences and events to promote Agilent products. Shuai serves as the resource to help R&D with new product development. In his leisure time, he likes exercising, hiking, cooking, gardening and traveling.

Tools and Techniques for Optimizing Cell Proliferation Studies

A review of cell proliferation assays, focusing on the choice of assay format, new advances and tips for optimization.

The Many Applications for Massively Parallel Oligonucleotide Libraries

Agilent’s leadership in Nucleic Acid Synthesis drives the fabrication of oligonucleotide libraries in a massively parallel way, independent of sequence. These oligo libraries power the research, development, and discovery in many fields of study. Key areas include protein design and engineering, disease therapeutics and testing, antibody engineering, genome and cell engineering, massively parallel reporter assays, enzyme engineering, gene assembly, and other novel applications. Join this session to learn more about Agilent’s capabilities in nucleic acid synthesis and hear a few application spotlights for how this product is being put to the test in the field today.