Biopharma Symposium 2021 Day 1

Summary

Rapid, Automated Sample Preparation for Protein Analysis -- Enrichment, Digestion, and Clean-up on a Single Platform

Protein analysis is a complex workflow. Today's highly sensitive LC/MS workflows are often hindered by the variability of the sample preparation methods, which are often antiquated, laborious, and imprecise. This short presentation will introduce the AssayMAP Bravo--a single platform for high-throughput, precise, and reproducible protein/peptide sample prep. We will discuss the components of the platform, the breadth of workflows covered, its ease-of-use, and share figures of merit demonstrating its robustness, sensitivity, and reproducibility. We will cover just a few of the key protein sample prep workflows, including enrichment, digestion, and clean-up for LC/MS analysis.

Presenter: Randy Bolger (Manager - Automation Solutions, North America, Agilent Technologies, Inc.)

Dr. Bolger manages sales of Agilent's Liquid-handling Automation portfolio for North America. He has over 25 years' experience developing and commercializing life science research tools for drug discovery and development. He holds a PhD in Biochemistry from the University of Wisconsin-Madison where his studies focused on protein biosynthesis and pharmacology. He has been involved in the development and utilization of AssayMAP as a protein sample prep platform since 2009.

Critical Quality Attribute (CQA) Characterization of mAbs utilizing tools designed for Biopharma

Protein biologics such as mAbs now represent a significant share of pharmaceutical sales and future growth potential, particularly in an era of increasing patent expirations. LC/MS is a powerful analytical method for characterizing therapeutic proteins. In this presentation, we will show the application of high resolution and high mass accuracy Q-TOF for intact mAb measurement, peptide mapping, and characterization of PTM (Post-Translation Modifications). Fast separation using AdvanceBio peptide mapping columns with a state-of-the-art HPLC system will also be highlighted.

Presenter: Lisa Sapp (Director Biopharma End Markets, Agilent Technologies, Inc.)

Lisa Sapp is the Global Director for Biopharma End Markets at Agilent Technologies. She has worked in the biopharmaceutical, pharmaceutical, medical device, clinical diagnostic, and biotechnology sectors with a combined experience of over 20 years. She has held roles in global business unit management, product management, market management, strategic marketing, business development, applications science and R&D. Lisa earned her Bachelor of Science in Microbiology and Cell Science from the University of Florida. She completed her Master of Science in Biochemistry at the University of Maryland with a focus in mass spectrometry. Lisa also has an M.B.A. in Marketing and Finance. Her interests include technology and method development for biologics characterization using state-of-the-art analytical tools.

Improving Resolution in your Aggregation Methods using the New Agilent 1290 Bio High Speed LC System

Monoclonal antibodies (mAb) are glycoproteins of the immunoglobulin (Ig) family. They can be used as therapeutics for the treatment of several life-threatening conditions such as cancer, inflammatory diseases, complications arising after organ transplantation, infectious, and cardiovascular diseases. Like all other protein therapeutics, mAbs can undergo aggregation during production, storage and transportation. Aggregation of therapeutic proteins is undesirable since it can lead to activity loss, decreased solubility, and enhanced immunogenicity. Usually, run under isocratic conditions, SEC method development is fairly straightforward. However, many analysts do not fully explore how changes in system volume effect sizing chromatography. Discover more about the new 1290 Bio LC and learn how minimizing dispersion volume improves resolution.

Presenter: Patrick Cronan (LC Applications Scientist, Agilent Technologies, Inc.)

Patrick Cronan is an LC applications Scientist with Agilent Technologies. He is an accomplished analytical chemist with over 20 years of experience in the pharmaceutical and Biopharma industries; has facilitated method transfer from HPLC to UHPLC while using LCMS to characterize large and small molecule drug-like compounds. Patrick has also worked extensively with Agilent’s 2D Chromatography system developing methods to increase throughput of biologic separations by coupling multiple techniques like SEC x IEX.