Confident and sensitive quantitation using data independent acquisition (DIA)
Learn how the Thermo Scientific Orbitrap Exploris 240 MS can maximize your Omics workflows, from the instrument capabilities itself to processing solutions to accelerate your path to confident results.
In this virtual series we will discuss proteomics quantitation workflows, such as LFQ, and data acquisition modes such DIA, PRM, and data analysis for proteomics applications. We will also cover metabolomics and lipidomics applications from method development, the use of Thermo Scientific Acquire X intelligent data acquisition workflow, and data analysis software solutions. All the RAW data presented will be available for download for offline processing.
Key learnings:
- Global proteome profiling with data independent acquisition
- Overview of the method (templates, parameters)
- Case studies utilizing Hela/E.coli mixed proteome experiments
- Case studies utilizing PRM targeted approach for sensitive, reproducible, and precise quantification of peptides
Presenter: Aaron Robitaille, Ph.D. (Sr. Product Marketing Manager, Thermo Fisher Scientific)
Dr. Robitaille has focused his career on using mass spectrometry to understand how eukaryotic cells dynamically and quantitatively respond to their environment to ensure proper cell growth and determine cell fate. He received his Ph.D. from the University of Basel in Switzerland utilizing a phosphoproteomics to identify a mechanism by which the conserved kinase mTOR regulated pyrimidine synthesis and promoted cell proliferation. He then completed his post-doctoral research at the University of Washington in Seattle, WA using quantitative proteomics to study how embryonic stem cells differentiated. Currently, Aaron Robitaille is a Senior Product Marketing Manager for Thermo Fisher Scientific in San Jose, CA. He is focused on supporting workflows that use differential ion mobility on a FAIMS Pro interface to help develop innovative mass spectrometry technology that answers challenging biological questions.
