Comprehensive adeno-associated virus critical quality attributes analysis with LC-MS
Adeno-associated viruses (AAVs) are the main viral vectors for gene therapy and have been successful in treating inherited retinal diseases and spinal muscular atrophy. An AAV is composed of an icosahedral protein shell with a single-stranded genome of approximately 4.7 kb. The intact AAVs act as a vehicle to protect and deliver oligonucleotide therapeutics. As AAVs continue to be explored as therapeutic delivery platforms, it is vital to ensure that all the critical quality attributes of the therapeutic product are maintained.
In this expert webinar, Dr. Wendi Hale, LC-MS applications scientist at Agilent Technologies, and Dr. Michelle English, customer success scientist at Protein Metrics, highlight a workflow to characterize AAVs at the intact and peptide level, including PTM identification, host cell protein analysis and sequence variants.
Key learning objectives
- Learn about the utility of mass spectrometry for AAV characterization
- How mass spectrometry at the intact and peptide level can confirm identification of AAV capsid proteins
- How AAV modifications such as PTMs and sequence variants can be identified by mass spectrometry
- How AAV characterization can be speeded up with good analytical tools and software
- How to correlate orthogonal techniques to fully characterize AAV
Who should attend?
- Scientists who are interested in learning more about AAV and the critical quality attributes that can be characterized with mass spectrometry
- Lab managers who need to speed up their analysis
- Analytical directors in biopharma
- CROs under time pressure to complete AAV analysis for their clients
- AAV manufacturers who need to learn how to characterize AAVs
- Vaccine developers
- Therapeutics developers and manufacturers
Presenter: Wendi Hale (LC/MS Applications Scientist, Agilent Technologies)
Wendi earned her PhD in analytical chemistry University of Michigan and did her postdoc at the University of Geneva. There she developed middle-down proteomics coupled with electron based MS/MS techniques for phosphoproteomics. Afterwards, Wendi joined Agilent Technologies as an applications scientist.
Presenter: Michelle English (Customer Success Scientist, Protein Metrics)
Michelle earned a degree in chemistry at the University of Colorado. This was followed by a PhD in bio analytical chemistry from The University of Virginia. Afterwards Michelle joined Pfizer as a senior scientist specialized in mass spectrometry before joining Protein Metrics as a customer success scientist.
Presenter: Moderator: Finn Price (Editorial Team, SelectScience)
Fionnbhar Price is a member of the SelectScience Editorial Team, who plays a core role in sourcing and publishing content for the site, with a particular focus on the field of applied chemistry. He has an MChem in Chemistry from Cardiff University, UK, and undertook a year in research characterizing bacterial protein.
