Resolve More Isoforms with the New Agilent 1290 Infinity II Bio LC System

Charge variant analysis is a demanding application for applied liquid chromatography systems due to the use of highly corrosive buffer salts in combination with very shallow gradients for optimal separation. (1) Reproducible gradient formation is essential for successful chromatography and the Agilent 1290 Infinity II Bio LC line combines a completely iron-free flow path with the functionality of binary or quaternary pumping systems.

The 1290 Bio High Speed pump offers excellent reproducibility for highly challenging shallow gradients and the 1290 Bio Flexible pump provides time saving method development features that can deliver exceptional resolution with minimal buffer preparation. The 1290 Infinity II Bio LC Systems, with a completely iron-free flow path, are optimally suited for the conditions used in biochromatography— avoiding potentially corrosive damage to the system, and excellent reproducibility confirms the 1290 Infinity II Bio LC as the next generation of Agilent high-end liquid chromatography systems for high confidence in generated data.

(1.) Schneider S., Agilent Technologies, How Shallow Can You Go? Refining charge variant analysis of mAbs with the Agilent 1290 Infinity II Bio LC System, Application Note 2020.

Presenter: Patrick Cronan (LC Applications Scientist, Agilent Technologies, Inc.)

Patrick Cronan is an LC applications Scientist with Agilent Technologies. He is an accomplished analytical chemist with over 20 years of experience in the pharmaceutical and Biopharma industries; has facilitated method transfer from HPLC to UHPLC while using LCMS to characterize large and small molecule drug-like compounds. Patrick has also worked extensively with Agilent’s 2D Chromatography system developing methods to increase throughput of biologic separations by coupling multiple techniques like SEC x IEX.