Robust and Reproducible Protein Quantification in Plasma using Evosep One and Agilent 6495 LC/TQ

Here we showcase the reproducibility, robustness, and analytical sensitivity of MRM-based LC/MS analysis of human plasma proteins using the Evosep One LC system and Agilent 6495 triple quadrupole LC/MS with an Agilent nanospray source. Results demonstrated excellent retention time reproducibility over a 12-day analysis period, and equivalent sensitivity before and after robustness testing, underlining the suitability of the system for high-throughput protein quantification when sample amounts were limited.

For Research Use Only. Not for use in diagnostic procedures.

Presenter: Linfeng Wu, PhD (LC/MS Application Scientist, Agilent Technologies, Inc.)

Dr. Linfeng Wu received her Ph.D. in 2007 at University of Connecticut for Biomedical Sciences. After a post-doc training in Dr. Michael Snyder’s group at Stanford University, she worked as a research scientist in biotech industry. Dr. Wu joined Agilent in 2015 as an application scientist, focusing on LC/MS-based analysis for protein, proteomics and small molecule analysis.