What’s Really Happening in Your Column: How Secondary Interactions Happen and What We Can Do about Them

Secondary interactions in HPLC are unintended interactions between the sample and any component of the flow path. They can lead to poor peak shape, poor resolution, and low sample recovery – and make it hard to interpret your chromatogram! An ideal separation is based purely on the primary separation mechanism, whether that is hydrophobicity in reversed-phase or size in size-exclusion chromatography. In reality, secondary interactions can happen in any kind of separation. In this webinar, we will discuss how they happen and how we can mitigate them, with tools such as mobile phase modifiers or inert LC column technology.

Presenter: Anne Blackwell, Ph.D. (Bio Columns Product Support Scientist, Agilent Technologies, Inc.)

Anne’s work in analytical chemistry began at Saint Louis University where she received Bachelor Degree in both Chemistry and French. She then began her career in mass spectrometry at the University of Arizona where she received her Ph.D. under Dr. Vicki Wysocki, studying mass spectrometry and ion mobility as tools for structural determination of non-covalent protein assemblies. She joined Agilent in 2012, working as an application scientist on LC/QTOF and LC/IM/QTOF instrumentation specializing in metabolomics and lipidomics applications. In 2016 she moved into Agilent’s bio-columns group, providing customer support for biological chromatography applications.