Overcoming Sample Preparation Challenges in Oligonucleotide LC-MS/MS Analysis
Oligonucleotide therapeutics have emerged as promising drug candidates due to their high specificity in targeting various genetic diseases and disorders. Despite advances in LC-MS, the inherent complexity, charge properties, and susceptibility to degradation make oligonucleotide quantitation in biological matrices particularly challenging. Effective sample preparation is essential to mitigate matrix effects, enhance analyte recovery, and ensure data reproducibility.
This seminar will discuss practical sample preparation strategies to improve matrix cleanup and extraction efficiency for oligonucleotides bioanalysis. We will demonstrate how these strategies achieve consistent recovery and strengthen overall method robustness.
By attending this webinar, you will learn:
- Gain practical insights into effective sample preparation strategies that enhance matrix cleanup, extraction efficiency, and recovery consistency in oligonucleotide bioanalysis, leading to more robust and reproducible LC-MS results.
Who should attend?
- Method development and bioanalytical scientists, laboratory managers, and CRO professionals involved in oligonucleotide drug discovery and quantitation workflows.
Presenter: Lee Williams (Director of R&D, Analytical Consumables and Applications, Biotage)
Lee Williams earned his PhD in mass spectrometry from the University of Wales, Swansea, and completed a Post-Doc in the Biochemical Toxicology department at the National Center for Toxicological Research, FDA labs. Lee joined Biotage (formerly Argonaut) to advance the LC/MS capability within the sample preparation arena. Over the 21 years, he has occupied a variety of roles, from senior scientist, group leader, lab manager, and, most recently, as the Director of R&D for Analytical Consumables and Applications.
