Peptide Analysis and Purification: From Fundamentals to Advanced Techniques
Key focus areas include:
- Impurity profiling
- Aggregation behavior
- Amino acid analysis (AAA)
The session begins with an introduction to peptides and their unique analytical challenges. From there, we will delve into practical strategies for method development, covering essential parameters like mobile phase selection, ion pairing, pH, and temperature optimization. Additionally, we will discuss column screening, highlighting the benefits of various chemistries, particle sizes, and pore dimensions. Finally, we will explore preparative techniques and purification workflows, offering tips for achieving high purity and recovery.
Whether you're optimizing existing methods or developing new ones, this session will equip you with the tools to streamline peptide characterization and purification.
Key learning objectives:
- Understand the importance of peptide impurity analysis and method optimization.
- Learn key strategies for column screening and method development.
- Discover how to achieve high purity and yield in peptide purification workflows.
Who should attend?
- Biopharma scientists involved in analytical development and quality control of peptide therapeutics.
- CRO’s and CDMO’s supporting the analysis or manufacturing of synthetic peptides.
- Academia and startups working on innovative peptide therapeutics.
Presenter: Katrien Sabbe, PhD (Biopharma Business Development Manager Chemistries and Supplies, Agilent Technologies)
Katrien Sabbe holds a PhD in organic synthesis (Ghent University, Belgium). For more than 25 years she is working in the field of Chromatography Columns and Supplies, first at VWR International and later at Varian Analytical Instruments, which was acquired in 2011 by Agilent Technologies. Currently she is the EMEA Biopharma Business Development Manager at Agilent Technologies Chemistries and Supplies Division.
