Eliminating Unwanted Variability in LC Methods: More Uptime, More Sample Throughput
The best HPLC method is one that produces reproducible analytical results under actual lab conditions. To produce results that are accurate and consistent over the long term the HPLC separation must either be relatively insensitive to small variations in analysis conditions, or those variations must be identified and controlled satisfactorily.
The development of a robust method is not only cost effective by reducing wasteful lab downtime, it also increases the likelihood of successful method transfer to other in-house operations, or contract analytical laboratories.
This presentation will:
- demonstrate how to determine the controlling parameters in a separation
- evaluate proper operating range of those parameters
- control strategy for those parameters
- facilitate faster method development and validation
By learning how to investigate and control the important factors in HPLC separations, attendees will be able to develop good, new methods for new products that are brought to market. As in any process that analyzes material for public consumption, improved analytical methods minimize unplanned downtime, increase lab efficiency and safe-guard the quality of those products. Less Downtime-More Sample Throughput.
Presenter: John Palmer (LC Column Application Engineer, Agilent Technologies, Inc.)
- B.S. degree in chemistry from the University of Cincinnati in 1977.
- Perkin Elmer GC and HPLC Specialist from 1984-1986.
- MAC-MOD Analytical in 1986 as a Chromatography Specialist for Zorbax HPLC Columns. His primary assignment was to aid customers in improving HPLC applications.
- Agilent Technologies in 2001 to continue to deliver chromatography support for the Agilent ZORBAX separation products.
John’s primary interest is enhancing the utility and robustness of HPLC/UHPLC separations by practical application of theory to everyday laboratory practice. His current interests are in high speed and high resolution separations of small molecules and bio-molecules utilizing both conventional HPLC and UHPLC technology.
He has collaborated on several papers and presentations including “Development and Validation of Analytical Methods in Pharmaceutical and Biomedical Research” for Pharmaceutical and Biomedical Applications of Liquid Chromatography (Riley, Lough, and Wagner).
